Reduced graphene oxide biosensor platform for the detection of NT-proBNP biomarker in its clinical range

Munief, Walid-Madhat; Lü, Xiaoling; Teucke, Tobias; Wilhelm, Jannick; Britz, Anette; Hempel, Felix; Lanche, Ruben; Schwartz, Miriam; Law, Jessica Ka-Yan; Grandthyll, Samuel; Müller, Frank; Neurohr, Jens-Uwe; Jacobs, Karin; Schmitt, Michael; Vivek, P.; Hempelmann, Rolf; Ingebrandt, Sven

doi:10.1016/j.bios.2018.09.102

Cited by 47 publications

(22 citation statements)

References 39 publications

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“…Meanwhile, the transfer characteristic curves (TCCs) (Figure 2B) indicate that all of the samples have the typical ambipolar characteristic. The close to zero charge neutral point (CNP, the crosses “+” in Figure 2B) in TCC of pure rGO (purple) is similar to the reported results in the literature [1], but is different from [14], which means that there is less oxygen residue. In terms of the curves’ contour, the scattering cross-sections of charged impurities for electrons and holes [15] and the contact of graphene with electrodes [16] are both likely to cause their asymmetry.…”

Section: Resultssupporting

confidence: 87%

“…Thereby, the currents (I DS ) changing tendencies in the h-branch and e-branch are asymmetric. It is also found that the locations of the curves are right-shifted from rGO (gray curves) to reduced GO&TAP (blue, magenta, and cyan curves are corresponding to n = 8, 4, 2, respectively) and comply with the p-type doping effect [1], which is deduced to be caused by the N elements in TAP. Meanwhile, the differences of the TCC’s moving direction in comparison with FGPC-decorated rGO-FET (which was left-shifted) could be ascribed to the opposite charges in FeTCP (negative) and TAP (positive) [14].…”

Section: Resultsmentioning

confidence: 99%

“…Reduced graphene oxide (rGO) is one of the welcomed two-dimensional and biofunctional materials for field effect transistor (FET)-type biosensors to fulfill the clinical detections [1,2], due to its balancing talent to recover the destroyed graphene basal plane in graphene oxide (GO) and preserve part of the oxygen-containing groups on it [3]. As a conductive channel material, research on the contact resistance between graphene and metal to improve the performance of the field effect transistor has achieved corresponding results [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Function of Tetra (4-Aminophenyl) Porphyrin in Altering the Electronic Performances of Reduced Graphene Oxide-Based Field Effect Transistor

Jia

2019

Molecules

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Porphyrin functionalized reduced graphene oxide (rGO) is attractive for multi-disciplinary research studies, and its improvements for an rGO-based field effect transistor (rGO-FET) were exploited to realize ultrasensitive biochemical and clinical assay. Although it was believed that the hybrids of porphyrin and rGO can make positive impacts on the rGO-FET’s electronic performances, the understandings of its functions are still piecemeal. Herein, the reduced mixtures of tetra (4-aminophenyl) porphyrin (TAP), GO (TAP-rGO), and the FET channeled by them are examined to throw a light on the possible approaches through which TAP affects rGO’s quality and its carrier mobilities. A TAP-caused game relationship is established by deliberating about the results of the intentionally altered experimental conditions, including TAP contents and the overmixing pretreatment. The p-type doping deduction for the right-shifted ambipolar transfer characteristic curves is evidenced by X-ray photoelectron spectroscopy (XPS). The problems posed by the TAP-induced FET features’ improvement, regression, and deterioration are clarified by the integrated proofs from Raman fingerprints, the amide and carboxyl groups’ changing trajectory found by C1s XPS core spectra, and the enlarged few-layer graphene morphology from atomic force microscope and transmission electron microscope. We hope that this effort will provide some constructive recommendations for producing low-cost graphene derivatives and promoting their applications in FET-like electronic components.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Function of Tetra (4-Aminophenyl) Porphyrin in Altering the Electronic Performances of Reduced Graphene Oxide-Based Field Effect Transistor

Jia

2019

Molecules

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“…The PtNP-decorated GFET immunosensors were then integrated with a custom-made microfilter and 400 nm pore polycarbonate membranes for blood-cell removal before delivering to fabricated GFET channels for BNP sensing, which also achieved an LOD at 100 fM in 0.001 × PBS and BNP concentration down to 50 nM in whole blood (Figure 7) [88]. Two years later, a research group from Germany surpassed this border with a rGO-ISFET device, which can detect the lowest concentration of NT-proBNP from 1 to 10 (pg/mL) with calculated LOD of 30 pg/mL, which is under the threshold value stage 1 of heart failure (100 pg/mL for men and 125 pg/mL for women according to the New York Heart Association) and permits diagnosis of health conditions [89].…”

Section: Antibody and Its Fragments As Bio-probes For Fet Immunosementioning

confidence: 99%

Field-Effect Transistor Biosensors for Biomedical Applications: Recent Advances and Future Prospects

Chen

2019

Sensors

172

117

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During recent years, field-effect transistor biosensors (Bio-FET) for biomedical applications have experienced a robust development with evolutions in FET characteristics as well as modification of bio-receptor structures. This review initially provides contemplation on this progress by analyzing and summarizing remarkable studies on two aforementioned aspects. The former includes fabricating unprecedented nanostructures and employing novel materials for FET transducers whereas the latter primarily synthesizes compact molecules as bio-probes (antibody fragments and aptamers). Afterwards, a future perspective on research of FET-biosensors is also predicted depending on current situations as well as its great demand in clinical trials of disease diagnosis. From these points of view, FET-biosensors with infinite advantages are expected to continuously advance as one of the most promising tools for biomedical applications.

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“…GO is chemically produced by oxidation and exfoliation of graphene, causing extensive oxidative modification of the basal plane [31, 75–77]. The rGO is prepared through reductive process of GO, for this purpose, different methods have been developed to reduce its oxygen content, including thermal, chemical, microwave, photochemical, microbial/bacterial, and photo-thermal methods [78–80]. GQDs consist of single to tens of layers of graphene with a size of a few nanometers which exhibit quantum phenomena [81, 82].…”

Section: Properties Of Graphene Materials In Biosensor Studymentioning

confidence: 99%

Graphene-based biosensors for the detection of prostate cancer protein biomarkers: a review

Wen

Pandit

et al. 2019

BMC Chemistry

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Prostate cancer (PC) is the sixth most common cancer type in the world, which causes approximately 10% of total cancer fatalities. The detection of protein biomarkers in body fluids is the key topic for the diagnosis and prognosis of PC. Highly sensitive screening of PC is the most effective approach for reducing mortality. Thus, there are a growing number of literature that recognizes the importance of new technologies for early diagnosis of PC. Graphene is playing an important role in the biosensor field with remarkable physical, optical, electrochemical and magnetic properties. Many recent studies demonstrated the potential of graphene materials for sensitive detection of protein biomarkers. In this review, the graphene-based biosensors toward PC analysis are mainly discussed in two groups: Firstly, novel biosensor interfaces were constructed through the modification of graphene materials onto sensor surfaces. Secondly, ingenious signal amplification strategies were developed using graphene materials as catalysts or carriers. Graphene-based biosensors have exhibited remarkable performance with high sensitivities, wide detection ranges, and long-term stabilities.

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Reduced graphene oxide biosensor platform for the detection of NT-proBNP biomarker in its clinical range

Cited by 47 publications

References 39 publications

Function of Tetra (4-Aminophenyl) Porphyrin in Altering the Electronic Performances of Reduced Graphene Oxide-Based Field Effect Transistor

Function of Tetra (4-Aminophenyl) Porphyrin in Altering the Electronic Performances of Reduced Graphene Oxide-Based Field Effect Transistor

Field-Effect Transistor Biosensors for Biomedical Applications: Recent Advances and Future Prospects

Graphene-based biosensors for the detection of prostate cancer protein biomarkers: a review

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