Reduced developmental potential of human oocytes after vitrification compared to slow freezing

Ragni, G.; Paffoni, A.; Alagna, Federica; Restelli, Liliana; Somigliana, Edgardo

doi:10.1016/j.fertnstert.2007.07.1161

Cited by 3 publications

(1 citation statement)

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“…To obtain the sample size of the study, a 50% increase of the cleavage rate on day 3 in the vitrification protocol (compared to slow freezing) was stated as relevant. The sample size was then calculated based on an expected cleavage rate of 45% in the slow freezing protocol (15) and setting type I and type II errors at .05 and .20, respectively. The number of required oocytes was thus about 90 per group.…”

Section: Statisticsmentioning

confidence: 99%

Developmental Potential of Human Oocytes After Slow Freezing or Vitrification: A Randomized In Vitro Study Based on Parthenogenesis

et al. 2008

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The aim of the this study was to compare the in vitro developmental competence of parthenogenetically activated oocytes cryopreserved with slow-freezing or vitrification. Supernumerary metaphase II oocytes obtained during in vitro fertilization procedures were randomized to slow freezing or vitrification procedure. After thawing or devitrification, oocytes were parthenogenetically activated and cultured. Survival, activation, development rate, and cell number during culture were compared. The 2 groups showed no significant differences between the rates of parthenogenetic activation, development, good quality parthenotes and blastomere number on day 2 of culture. However, parthenotes from the devitrified oocytes continued cleaving till day 3 in a significantly low proportion (27% vs. 42%). On day 3, the mean number of blastomeres was also lower in vitrification group compared to slow-freezing (4.8 + 1.9 vs. 5.8 + 1.7). In conclusion, parthenogenesis highlights a reduced potential of vitrified oocytes to cleave on day 3 compared with oocytes from slow-freezing.

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Section: Statisticsmentioning

confidence: 99%