Reduced allergenicity of high molecular weight ragweed polymers

Patterson, Roy; Suszko, Irena M.; Bacal, Edita; Zeiss, C. Raymond; Kelly, Joseph F.; Pruzansky, Jacob J.

doi:10.1016/0091-6749(79)90161-1

Cited by 25 publications

(6 citation statements)

References 5 publications

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“…Patterson et al [3][4][5][6][7] have produced allergen derivatives from several pollen species by using glutaraldehyde as the modify ing agent. The modulated immunologic properties of their products were attributed to the degree of polym erization with molecular weight range of 200,000 to 20,000,000.…”

Section: Introductionmentioning

confidence: 99%

Studies on Allergen and Allergoid Preparations from Purified Timothy <i>(Phleum pratense)</i> Pollen Extracts

Puttonen

Maasch²,

Pilström

1982

Int Arch Allergy Immunol

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Physicochemical and in vitro allergenic properties of two different preparations of timothy (Phleum pratense) pollen were compared. The refined allergen extract, timothy B, contained proteins of molecular weights from 10,000 to 45,000 daltons. The allergoid preparation, which was prepared by mild treatment of the partially purified extract with formaldehyde, possessed only a trace of activity in the RAST inhibition assay. The formaldehyde treatment also resulted in a shift of the net charge of proteins to the more acidic site, as demonstrated by isoelectric focusing. Furthermore, it was shown that the activities of naturally occurring enzymes of native allergen extracts were reduced considerably. Only traces of acid phosphatase activity could be demonstrated in timothy B allergoid.

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Section: Introductionmentioning

confidence: 99%

Studies on Allergen and Allergoid Preparations from Purified Timothy <i>(Phleum pratense)</i> Pollen Extracts

Puttonen

Maasch²,

Pilström

1982

Int Arch Allergy Immunol

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“…The above peculiarity of KCNO-treated allergens allows the possibility to distinguish monomeric from polymeric allergoids such as those obtained by the reaction of allergens with glutaraldehyde [19, 20]. Polymeric allergoids are characterized by a significant increase in the molecular size of their components (reaching more than 1,000 kDa) as result of inter-/intramolecular cross-linking.…”

Section: Discussionmentioning

confidence: 99%

“…Such allergoids are characterized by a significant reduction in allergenic potency (probably as the result of an alteration in IgE epitopes of allergenic molecules) while still maintaining their immunogenic properties (capacity to induce IgG antibodies able to interact with native extract). Furthermore, unlike polymeric allergoids (such as those obtained by reaction with aldehydes) which are characterized by a strong increase in molecular dimension of their components due to inter-/intramolecular cross-linking phenomena [19, 20], they maintain a native molecular (monomeric) dimension of their own components [18]. …”

Section: Introductionmentioning

confidence: 99%

Date Palm Pollen Allergoid: Characterization of Its Chemical-Physical and Immunological Properties

Mistrello

Harfi

Roncarolo

et al. 2007

Int Arch Allergy Immunol

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Background: Date palm (DP) pollen can cause allergic symptoms in people living in different countries. Specific immunotherapy with allergenic extracts by subcutaneous route is effective to cure allergic people. However, the risk of side effects has led to explore safer therapeutic modalities. The aim of our work was to evaluate IgE cross-reactivity between DP and autochthonous palm (European fan palm, EFP) pollen extracts, to chemically modify DP extract with potassium cyanate in order to obtain an allergoid, and to characterize it. Methods: By radioallergosorbent test inhibition, immunoblotting (IB) and skin prick test, in vitro and in vivo allergenic activities of native and modified DP extracts were compared. By SDS-PAGE and IB, we compared the protein profile and IgE-binding capacity of both native and modified DP, as well as of EFP extracts. By IB inhibition, IgE cross-reactivity of native DP and EFP extracts was evaluated. By ELISA, the capacity of modified DP-induced IgG to react with native DP extract was determined. Results: Radioallergosorbent test inhibition, IB and skin prick test results demonstrated that modified DP was significantly less allergenic than native DP extract. The SDS-PAGE profile showed that potassium cyanate treatment of DP extract did not alter the molecular weight of its components. In addition, no difference was observed between native DP and EFP extracts. Subsequent IB inhibition data evidenced the existence of a strong IgE cross-reactivity between native DP and EFP extracts. ELISA results indicated that the administration of modified DP in mice was able to induce specific IgG also recognizing native DP extract. Conclusions: Modified DP extract (allergoid) seems to be a good candidate for immunotherapy of patients affected by specific allergy.

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“…Polymerization by glutaraldehyde. By treatment with glutaraldehyde aldehyde groups of glutaraldehyde react with the amino groups of protein resulting in cross-linked allergen proteins with altered immunological characteristics (Patterson et al, 1979). Recently the ability of glutaraldehyde-treated allergens to stimulate T-cells has been disputed (Wurtzen et al, 2007).…”

Section: Covalent Modificationsmentioning

confidence: 99%