1982
DOI: 10.1159/000233058
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Studies on Allergen and Allergoid Preparations from Purified Timothy <i>(Phleum pratense)</i> Pollen Extracts

Abstract: Physicochemical and in vitro allergenic properties of two different preparations of timothy (Phleum pratense) pollen were compared. The refined allergen extract, timothy B, contained proteins of molecular weights from 10,000 to 45,000 daltons. The allergoid preparation, which was prepared by mild treatment of the partially purified extract with formaldehyde, possessed only a trace of activity in the RAST inhibition assay. The formaldehyde treatment also resulted in a shift of the net charge of proteins to the … Show more

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Cited by 16 publications
(10 citation statements)
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“…If compared on the basis of pro tein content approximately 90 times more protein of GOID than of GEN was necessary to elicit mean wheal sizes of 1 HEP. These results were in the same range as found in previous studies in PCA and ana phylactic studies [4], These findings suggest that dif ferences in allergenic activity of GEN and GOID can also be detected in vivo. However, in vivo assays yielded different activity ratios as in vitro assays.…”
Section: Discussionsupporting
confidence: 90%
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“…If compared on the basis of pro tein content approximately 90 times more protein of GOID than of GEN was necessary to elicit mean wheal sizes of 1 HEP. These results were in the same range as found in previous studies in PCA and ana phylactic studies [4], These findings suggest that dif ferences in allergenic activity of GEN and GOID can also be detected in vivo. However, in vivo assays yielded different activity ratios as in vitro assays.…”
Section: Discussionsupporting
confidence: 90%
“…In two previous papers [3,4], we could demon strate that formaldehyde treatment of native allergen extracts yielded products with the properties of allergoids [5], In several in vitro and in vivo assays the al lergenicity of allergens versus allergoids was dimin ished markedly. In RAST inhibition and histamine release from washed human leukocytes the residual allergenic activity of GOID was 0.1% compared to the activity of GEN.…”
Section: Discussionmentioning
confidence: 99%
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“…Their characteristic feature is minimal IgE-binding activity but retained immunogenicity, documented in both animals and humans (3)(4)(5)(6)(7)(8)(9)(10). Few data are available that directly show the T cell-stimulating capacity of allergoids.…”
mentioning
confidence: 99%