Redox regulation of the actin cytoskeleton and its role in the vascular system

Xu, Qian; Huff, Lauren P.; Fujii, Masakazu; Griendling, Kathy K.

doi:10.1016/j.freeradbiomed.2017.03.004

Cited by 88 publications

(66 citation statements)

References 424 publications

(495 reference statements)

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“…Indeed, cytoskeletal behaviors, under ROS stress, is widely shown to be altered (Raghu et al, 1986 ; Zhao and Davis, 1998 ; Gellert et al, 2015 ; Wilson and González-Billault, 2015 ; Xu et al, 2017 ), including actin polymerization, filament assembly, branching, cytoskeletal reorganization, and dynamic interactions with cytoskeletal regulators. These behavioral changes stem from either the direct oxidation of actin by ROS, or the ROS-initiated signaling, or both.…”

Section: Ros Are Required To Form Intercellular/interorganelle Bridgementioning

confidence: 99%

A Salutary Role of Reactive Oxygen Species in Intercellular Tunnel-Mediated Communication

Liang

2018

Front. Cell Dev. Biol.

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The reactive oxygen species, generally labeled toxic due to high reactivity without target specificity, are gradually uncovered as signaling molecules involved in a myriad of biological processes. But one important feature of ROS roles in macromolecule movement has not caught attention until recent studies with technique advance and design elegance have shed lights on ROS signaling for intercellular and interorganelle communication. This review begins with the discussions of genetic and chemical studies on the regulation of symplastic dye movement through intercellular tunnels in plants (plasmodesmata), and focuses on the ROS regulatory mechanisms concerning macromolecule movement including small RNA-mediated gene silencing movement and protein shuttling between cells. Given the premise that intercellular tunnels (bridges) in mammalian cells are the key physical structures to sustain intercellular communication, movement of macromolecules and signals is efficiently facilitated by ROS-induced membrane protrusions formation, which is analogously applied to the interorganelle communication in plant cells. Although ROS regulatory differences between plant and mammalian cells exist, the basis for ROS-triggered conduit formation underlies a unifying conservative theme in multicellular organisms. These mechanisms may represent the evolutionary advances that have enabled multicellularity to gain the ability to generate and utilize ROS to govern material exchanges between individual cells in oxygenated environment.

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Section: Ros Are Required To Form Intercellular/interorganelle Bridgementioning

confidence: 99%

A Salutary Role of Reactive Oxygen Species in Intercellular Tunnel-Mediated Communication

Liang

2018

Front. Cell Dev. Biol.

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“…A change in the redox environment within a cell can trigger redox sensitive signaling cascades [ 5 ]. Redox sensitive thiols present on proteins can act as regulatory switches [ 5 – 8 ] and activate cell signaling cascades leading to modifications in cellular function at multiple levels from regulating the actin cytoskeleton [ 9 ] to the level of transcription [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

LSD1 mediated changes in the local redox environment during the DNA damage response

et al. 2018

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The redox state of the cell can be affected by many cellular conditions. In this study we show that detectable reactive oxygen species (ROS) are also generated in response to DNA damage by the chromatin remodeling factor and monoamine oxidase LSD1/KDM1A. This raised the possibility that the localized generation of hydrogen peroxide produced by LSD1 may affect the function of proximally located DNA repair proteins. The two major pathways for repair of DNA double-strand breaks (DSBs) are homologous recombination (HR) and non-homologous end joining (NHEJ). Cells were exposed to low levels of ectopic H2O2, DNA breaks generated by laser light, and recruitment kinetics of NHEJ protein Ku80 to DNA damage sites determined. Ku80 recruitment to damage sites was significantly decreased in cells pretreated with H2O2 while HR end binding protein Nbs1 was increased. This suggests that the DNA repair pathway choice has the potential to be modulated by the local redox state. This has implications for chemotherapeutic approaches involving generating DNA damage to target actively dividing cancer cells, which may be more or less effective dependent on the redox state of the targeted cells and the predominant repair pathway required to repair the type of DNA damage generated.

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“…The function of these iron–sulfur flavoprotein homologs is unknown but may be involved in electron transfer, which would allow DPI to selectively deplete T. vaginalis intracellular ATP levels. Given that actin reorganization and general cytoadherence require a large consumption of ATP, this may be responsible for T. vaginalis detachment [ 39 ]. Interestingly, DPI did not induce T. vaginalis cell death in our study.…”

Section: Discussionmentioning

confidence: 99%

Adherence of Trichomonas vaginalis to SiHa Cells is Inhibited by Diphenyleneiodonium

et al. 2020

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Microbial adhesion is critical for parasitic infection and colonization of host cells. To study the host–parasite interaction in vitro, we established a flow cytometry-based assay to measure the adherence of Trichomonas vaginalis to epithelial cell line SiHa. SiHa cells and T. vaginalis were detected as clearly separated, quantifiable populations by flow cytometry. We found that T. vaginalis attached to SiHa cells as early as 30 min after infection and the binding remained stable up to several hours, allowing for analysis of drug treatment efficacy. Importantly, NADPH oxidase inhibitor DPI treatment induced the detachment of T. vaginalis from SiHa cells in a dose-dependent manner without affecting host cell viability. Thus, this study may provide an understanding for the potential development of therapies against T. vaginalis and other parasite infections.

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Redox regulation of the actin cytoskeleton and its role in the vascular system

Cited by 88 publications

References 424 publications

A Salutary Role of Reactive Oxygen Species in Intercellular Tunnel-Mediated Communication

A Salutary Role of Reactive Oxygen Species in Intercellular Tunnel-Mediated Communication

LSD1 mediated changes in the local redox environment during the DNA damage response

Adherence of Trichomonas vaginalis to SiHa Cells is Inhibited by Diphenyleneiodonium

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