Redox Capacitive Assaying of C-Reactive Protein at a Peptide Supported Aptamer Interface

Piccoli, Julia P.; Hein, Robert; El‐Sagheer, Afaf H.; Brown, Tom; Cilli, Eduardo Maffud; Bueno, Paulo Agenor Alves; Davis, Jason J.

doi:10.1021/acs.analchem.7b05374

Cited by 65 publications

(51 citation statements)

References 57 publications

(122 reference statements)

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“…The linear detection range between 10 pM and 10 nM facilitates measurements within the clinically useful range for CRP [14]. The limits of detection of 4.0 and 1.6 pM (0.265 and 0.192 ng/mL) for the BSA and CRP receptive interfaces, respectively, compare favourably with previous reports of label‐free electrochemical CRP detection methodologies [6a, 7b, d] and show higher sensitivity than previously reported for traditional detection of CRP by ELISA (58 pM, 7 ng/mL) [15].…”

Section: Methodssupporting

confidence: 74%

Label‐free Selective Detection of Protein Markers in the Picomolar Range via a Convenient Voltammetric Sensing Strategy

Chen

Lehr

2021

Electroanalysis

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A convenient strategy for the preparation of label‐free selective protein biomarker sensors is presented. After formation of a ferrocene terminated monolayer on a gold electrode using thiol chemistry, grafting of an antifouling phenylalanine layer onto the preformed ferrocene monolayer is achieved by diazonium chemistry. This provides a platform for receptor (anti‐BSA or anti‐CRP) immobilization as well an antifouling interface. Specific target binding leads to attenuation of the voltammetric faradaic signal of the underlying ferrocene group. Thereby, label‐free, selective, voltammetric detection is achieved with highly competitive limits of detection in the low (≤4) picomolar range for both BSA and CRP.

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Section: Methodssupporting

confidence: 74%

Label‐free Selective Detection of Protein Markers in the Picomolar Range via a Convenient Voltammetric Sensing Strategy

Chen

Lehr

2021

Electroanalysis

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“…2b). In order to enable selective CRP detection, we chose two well-characterised CRP-DNA aptamers (aptamer 1 and aptamer 2) from the literature [41][42][43] as potential capture moieties. We note that while the sequences of the DNA aptamers employed here are the same as the ones published, for this application the aptamers have to be extended at their 5′ or 3′ end, respectively, so they can be hybridised into the cavity.…”

Section: Dna Nanostructures As Translocation Carriers In Biosensingmentioning

confidence: 99%

Rational design of DNA nanostructures for single molecule biosensing

et al. 2020

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The ability to detect low concentrations of biomarkers in patient samples is one of the cornerstones of modern healthcare. In general, biosensing approaches are based on measuring signals resulting from the interaction of a large ensemble of molecules with the sensor. Here, we report a biosensor platform using DNA origami featuring a central cavity with a target-specific DNA aptamer coupled with a nanopore read-out to enable individual biomarker detection. We show that the modulation of the ion current through the nanopore upon the DNA origami translocation strongly depends on the presence of the biomarker in the cavity. We exploit this to generate a biosensing platform with a limit of detection of 3 nM and capable of the detection of human C-reactive protein (CRP) in clinically relevant fluids. Future development of this approach may enable multiplexed biomarker detection by using ribbons of DNA origami with integrated barcoding.

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“…Heterogeneous SAMs can also be formed by mixing different biomolecules, in particular peptides and aptamers, allowing for the design of highly sensitive platforms [41]. An interface composed of a redox-tagged peptide, integrated with receptive aptamers, was developed to target C-reactive protein (CRP), a biomarker of systemic inflammation [42]. The study was further extended by designing peptides that could attach to antibodies instead of aptamers and achieve good sensitivity for clinically relevant levels of CRP [16].…”

Section: Peptides As Self-assembled Layersmentioning

confidence: 99%

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

et al. 2021

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Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.

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Redox Capacitive Assaying of C-Reactive Protein at a Peptide Supported Aptamer Interface

Cited by 65 publications

References 57 publications

Label‐free Selective Detection of Protein Markers in the Picomolar Range via a Convenient Voltammetric Sensing Strategy

Label‐free Selective Detection of Protein Markers in the Picomolar Range via a Convenient Voltammetric Sensing Strategy

Rational design of DNA nanostructures for single molecule biosensing

The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

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