Rediscovery of <i>Ardisia gigantifolia</i> and the reinstatement of <i>A. kteniophylla</i> (Primulaceae) – finding the correct name for the Chinese medicinal plant ’Zou Ma Tai’

Huang, Ge-Han; Hu, Chi‐Ming; Hao, Gang

doi:10.1111/njb.01672

Cited by 4 publications

(3 citation statements)

References 6 publications

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“…Ardisia gigantifolia Stapf (Primulaceae) is a critically endangered subshrub endemic to the Yunnan Province of China (Qin et al 2017) and its scientific name has long been confused with the Chinese medicinal plant 'Zou Ma Tai' (Huang et al 2017). Most species in Ardisia are used for medicinal purposes, suggesting that A. gigantifolia would have potential medicinal value (Liu et al 2018).…”

Section: Introductionmentioning

confidence: 99%

The physiological responses of critically endangered species Ardisia gigantifolia Stapf (Primulaceae) to different light intensities

LIU¹,

NING²,

LI³

et al. 2023

Photosynt.

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To investigate the light intensity suitable for the growth of Ardisia gigantifolia Stapf, morphology, photosynthetic parameters, and indicators of oxidative stress were analyzed under different light intensities. Compared to highirradiance treatment, medium and low-irradiance treatments promoted plant growth and restricted transpiration. Compared to medium irradiance, plants under high and low irradiance exhibited significantly lower maximal photochemical efficiency, potential photochemical efficiency, and electron transport rate, but significantly higher malondialdehyde content. This indicated that both excessive light and severe shading inhibited photosynthetic activity and induced oxidative stress, which resulted in a significant decrease in net photosynthetic rate. A. gigantifolia can adapt to different light intensities, improving light harvesting and utilizing capacity under low irradiance by increasing Chl (a+b) content and reducing Chl a/b ratio, and adapting to high irradiance by enhancing heat dissipation and activity of peroxidase. A. gigantifolia showed the best performance in growth and photosynthesis under medium irradiance treatment. Highlights• Medium irradiance was the optimum light intensity for growth and photosynthesis in Ardisia gigantifolia • High and low irradiance inhibited photosynthesis in A. gigantifolia • A. gigantifolia adapted to excessive irradiance and light deficiency through physiological regulation

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Section: Introductionmentioning

confidence: 99%

The physiological responses of critically endangered species Ardisia gigantifolia Stapf (Primulaceae) to different light intensities

LIU¹,

NING²,

LI³

et al. 2023

Photosynt.

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“…Ardisia kteniophylla A. DC, an evergreen shrub from the genus Ardisia (Primulaceae family), was mainly found in South China, North Vietnam, Laos and Thailand, and commonly known as a traditional folk medicine to treat rheumatism and injuries from muscles and fractures in China (Guan et al, 2016;Huang et al, 2017). It was first documented in the classical medical book "Sheng Cao Yao Xing Bei Yao" in the Qing dynasty (written in 1711), generally utilized by ethnic groups such as Yao, Zhuang, Dong, Jing, Li, Miao (Tian et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Systematic identification of reference genes for qRT-PCR of Ardisia kteniophylla A. DC under different experimental conditions and for anthocyanin-related genes studies

Wang,

Zhang,

et al. 2023

Front. Plant Sci.

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Ardisia kteniophylla A. DC, widely known as folk medicinal herb and ornamental plant, has been extensively investigated due to its unique leaf color, anti-cancer and other pharmacological activities. The quantitative real-time PCR (qRT-PCR) was an excellent tool for the analysis of gene expression with its high sensitivity and quantitative properties. Normalizing gene expression with stable reference genes was essential for qRT-PCR accuracy. In addition, no studies have yet been performed on the selection, verification and stability of internal reference genes suitable for A. kteniophylla, which has greatly hindered the biomolecular researches of this species. In this study, 29 candidate genes were successfully screened according to stable expression patterns of large-scale RNA seq data that from a variety of tissues and the roots of different growth stages in A. kteniophylla. The candidates were then further determined via qRT-PCR in various experimental samples, including MeJA, ABA, SA, NaCl, CuSO4, AgNO3, MnSO4, CoCl2, drought, low temperature, heat, waterlogging, wounding and oxidative stress. To assess the stability of the candidates, five commonly used strategies were employed: delta-CT, geNorm, BestKeeper, NormFinder, and the comprehensive tool RefFinder. In summary, UBC2 and UBA1 were found to be effective in accurately normalizing target gene expression in A. kteniophella regardless of experimental conditions, while PP2A-2 had the lowest stability. Additionally, to verify the reliability of the recommended reference genes under different colored leaf samples, we examined the expression patterns of six genes associated with anthocyanin synthesis and regulation. Our findings suggested that PAP1 and ANS3 may be involved in leaf color change in A. kteniphella. This study successfully identified the ideal reference gene for qRT-PCR analysis in A. kteniphella, providing a foundation for future research on gene function, particularly in the biosynthesis of anthocyanins.

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“…Ardisia kteniophylla A. DC. is a perennial plant within the Primulaceae family (Ericales; Huang et al, 2017), and its roots are widely known to accumulate pharmacologically active products such as saponins, benzoquinones, flavanones, and sterols (Sun, Li, et al, 2017; Sun, Jiang, et al, 2017; Zhou, 2017). These products likely form the biochemical basis for the utility of A .…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic analysis of genes in the triterpene saponin biosynthesis pathway in leaves and roots of Ardisia kteniophylla A. DC., a plant used in traditional Chinese medicine

Lei

Harris

Wang

et al. 2022

Ecology and Evolution

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Ardisia kteniophylla (Primulaceae) is highly valued in traditional medicine due to its production of the pharmacologically active secondary metabolites, especially triterpenoid saponins in its roots. Although A. kteniophylla is very important in traditional medicine, the genetic basis for its production of triterpenoid saponins remains largely unknown. Therefore, we sequenced transcriptomes of A. kteniophylla to identify putative genes involved in production of triterpenoid saponins in both leaves and roots, and we used the transcriptomes to compare expression levels of these genes between the two organ systems. The production of triterpenoid saponins in plants is usually induced through hormonal signaling on account of the presence of pests.Thus, we treated plants with the hormones salicylic acid (SA) and methyl jasmonate (MeJA) and used quantitative real-time PCR (qRT-PCR) to investigate expression levels of genes involved in triterpenoid saponin biosynthesis. In total, we obtained transcriptomes for leaf and root tissues representing 52,454 unigenes. Compared with the leaf transcriptome, we found that 6092 unigenes were upregulated in the root, especially enzymes involved in the direct synthesis of triterpenoid saponins, while 6001 genes appeared downregulated, including those involved in precursory steps in the triterpenoid saponin biosynthesis pathway. Our results from qRT-PCR indicate that genes within the upstream parts of the triterpenoid saponin biosynthesis pathway may be upregulated under exposure to the applied hormones, but downstream genes are downregulated. This suggests possible conflicting effects of SA and MeJA in promoting the production of secondary metabolites on the one hand, and, on the other, limiting plant growth processes to devote energy to combating pests. We also performed an analysis of transcription factors (TFs) and found 997 unique transcripts belonging to 16 TF families. Our data may help to facilitate future work on triterpene saponins biosynthesis in A. kteniophylla with potential pharmacological and molecular breeding applications.

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Rediscovery of Ardisia gigantifolia and the reinstatement of A. kteniophylla (Primulaceae) – finding the correct name for the Chinese medicinal plant ’Zou Ma Tai’

Cited by 4 publications

References 6 publications

The physiological responses of critically endangered species Ardisia gigantifolia Stapf (Primulaceae) to different light intensities

The physiological responses of critically endangered species Ardisia gigantifolia Stapf (Primulaceae) to different light intensities

Systematic identification of reference genes for qRT-PCR of Ardisia kteniophylla A. DC under different experimental conditions and for anthocyanin-related genes studies

Comparative transcriptomic analysis of genes in the triterpene saponin biosynthesis pathway in leaves and roots of Ardisia kteniophylla A. DC., a plant used in traditional Chinese medicine

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