Red wine antioxidant resveratrol‐modified cardiac stem cells regenerate infarcted myocardium

Abstract

To study the efficiency of maintaining the reduced tissue environment via pre-treatment with natural antioxidant resveratrol in stem cell therapy, we pre-treated male Sprague-Dawley rats with resveratrol (2.5 mg/kg/day gavaged for 2 weeks). After occlusion of the left anterior descending coronary artery (LAD), adult cardiac stem cells stably expressing EGFP were injected into the border zone of the myocardium. One week after the LAD occlusion, the cardiac reduced environment was confirmed in resveratrol-treated rat hearts by the enhanced expression of nuclear factor-E2-related factor-2 (Nrf2) and redox effector factor-1 (Ref-1). In concert, cardiac functional parameters (left ventricular ejection fraction and fractional shortening) were significantly improved. The improvement of cardiac function was accompanied by the enhanced stem cell survival and proliferation as demonstrated by the expression of cell proliferation marker Ki67 and differentiation of stem cells towards the regeneration of the myocardium as demonstrated by the enhanced expression of EGFP 28 days after LAD occlusion in the resveratrol-treated hearts. Our results demonstrate that resveratrol maintained a reduced tissue environment by overexpressing Nrf2 and Ref-1 in rats resulting in an enhancement of the cardiac regeneration of the adult cardiac stem cells as demonstrated by increased cell survival and differentiation leading to cardiac function.

In vitro antitumor activity of chemical constituents of EtOAc extract from Artemisa gmelinii

“…Diphenylethene is a group of natural organic compounds with a C 6 -C 2 -C 6 unit in the parent nucleus, which have a variety of biological activities, such as anti-tumor, antihypertensive, ester, antiplatelet aggregation, antibacterial and so on. For example, resveratrol is a widely known natural product, which exhibited significant pharmacological activities [23][24][25][26] and considered a plant antitoxin. The structure of compound 5 can be regarded as the oxidized and cyclization of the vinyl group (C 2 ) in diphenylethene.…”

Resveratrol: A miraculous natural compound for diseases treatment

“…Both antioxidant activity and increasing of nitric oxide bioavailability actions of RSV are required to exert its anti‐ischemic effects (Leikert et al., 2002). Studying of cardiac stem cells demonstrated that RSV could potentiate the regeneration of infarcted myocardium (Gurusamy, Ray, Lekli, & Das, 2010). Recently, several lines of evidences have shown the role of micro‐RNAs (miR‐21, miR‐20b, miR‐27a, miR‐9, miR‐29) in cardio‐protective effects of RSV (Mukhopadhyay, Pacher, & Das, 2011).…”

El resveratrol protege contra la respuesta inflamatoria en páncreas secundaria al envejecimiento

“…En modelos animales se ha demostrado que la administración de resveratrol puede prolongar la esperanza de vida (Valenzano et al, 2006) debido a sus efectos de protección cardiovascular en modelos de lesión miocardio (Hattori et al, 2002;Juric et al, 2007;Gurusamy et al, 2010) hipertensión (Juric et al, 2007;) y diabetes tipo 2 (Zhang et al, 2009;Zhang et al,2010).…”