Red-emitting protein-coated conjugated polymer nanoparticles

Peters, Ruby; Sandiford, Lydia; Owen, Dylan M.; Kemal, Evren; Bourke, Struan; Dailey, Lea Ann; Green, Mark A.

doi:10.1039/c6pp00160b

Cited by 14 publications

(9 citation statements)

References 18 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrophobins can be introduced during particle generation, e.g., as stabilizers during miniemulsification. In the future, hydrophobins could be ligated with biological recognition motifs to allow binding of the CPNs to the pathological site …”

Section: Approaches For Surface Functionalization and Targetingmentioning

confidence: 99%

Conjugated Polymer Nanoparticles toward In Vivo Theranostics – Focus on Targeting, Imaging, Therapy, and the Importance of Clearance

Kuehne

2017

Adv. Biosys.

View full text Add to dashboard Cite

Conjugated polymer nanoparticles are highly fluorescent colloids with tunable emission colors ranging from the visible deep into the near infrared spectrum. Conjugated polymer nanoparticles are easy to prepare, tunable in their size, and virtually nonbleachable. Conjugated polymer particles can also be designed to give off heat upon irradiation. All these properties make conjugated polymer particles ideal materials for biomedical fluorescence and photoacoustic imaging as well as for theranostic applications. Here, different examples of surface functionalization to attach pathological homing devices, imaging modalities, as well as the emerging possibilities for therapeutic measures are discussed. Furthermore, clearance of the particles is considered, which is important to ultimately apply the materials for in vivo theranostics. Due to the conjugated backbone of the conjugated polymers, established degradation strategies, as known from hydrophilic nonconjugated polymer carriers, cannot be applied. Bioinspired strategies and potential pathways for degradation and clearance via structural changes upon triggers such as pH, oxidation, and temperature are also discussed in this progress report.

show abstract

Section: Approaches For Surface Functionalization and Targetingmentioning

confidence: 99%

Conjugated Polymer Nanoparticles toward In Vivo Theranostics – Focus on Targeting, Imaging, Therapy, and the Importance of Clearance

Kuehne

2017

Adv. Biosys.

View full text Add to dashboard Cite

show abstract

“…Besides facilitating the formation of nanoparticles and ensure their colloidal stability, coating agents may also be used to provide a neutral charge to CPNs 133 and to enable bioconjugation with targeting molecules 92,133 . Overall, CPNs have been prepared with varied material composition, which includes lipids 3,19,88,93,134 proteins 100,135 , polymers 94,132,136 and surfactants 85,97 .…”

Section: Nanoprobes Of Conjugated Polymersmentioning

confidence: 99%

Conjugated polymers as nanoparticle probes for fluorescence and photoacoustic imaging

et al. 2020

View full text Add to dashboard Cite

show abstract

“…All fluorophores investigated in this study absorb and emit in the NIR region, which is a prerequisite for in vivo optical imaging applications, due to the transparency of most biological tissues between 600-1100 nm 36 . It has been frequently observed that fluorophore encapsulation within different nanoparticle matrix materials can dramatically influence the optical properties 15,20 .…”

Section: Optical Characterisation Of Fluorophore-loaded Nanoparticlesmentioning

confidence: 99%

In Vivo Optical Performance of a New Class of Near-Infrared-Emitting Conjugated Polymers: Borylated PF8-BT

Neumann

Crossley

Turner

et al. 2019

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Borylated polyfluorenes are π-conjugated polymers (CPs) with near infrared (NIR) absorption and emission profiles suitable for in vivo optical imaging. A fully borylated polyfluorene derivative (P4) was encapsulated in PEG-PLGA nanoparticles and compared with a reference NIR-emitting CP (PCPDTBT) or indocyanine green (ICG). All formulations satisfied quality requirements for parenterally administered diagnostics. P4 nanoparticles had higher quantum yield (2.3%) than PCPCDTBT (0.01%) or ICG nanoparticles (1.1%). The signal:background ratios (SBR) of CP systems in a phantom mouse (λem=820 nm) increased linearly with fluorophore mass (0.1-1 µg), while ICG showed quenching above 0.25 µg. P4 nanoparticles experienced < 10% photobleaching over 10 irradiations (PCPDTBT: ~25% and ICG: >44%). In a mouse tumour xenograft model, P4 nanoparticles showed a 5-fold higher SBR than PCPDTBT with fluorophore accumulation in the liver > spleen > tumour. Blood chemistry and tissue histology showed no abnormalities compared to untreated animals after a single administration.

show abstract

Red-emitting protein-coated conjugated polymer nanoparticles

Abstract: Red emitting materials are desirable in biology due to the transparency of certain biological tissues at these wavelengths.

Cited by 14 publications

References 18 publications

Conjugated Polymer Nanoparticles toward In Vivo Theranostics – Focus on Targeting, Imaging, Therapy, and the Importance of Clearance

Conjugated Polymer Nanoparticles toward In Vivo Theranostics – Focus on Targeting, Imaging, Therapy, and the Importance of Clearance

Conjugated polymers as nanoparticle probes for fluorescence and photoacoustic imaging

In Vivo Optical Performance of a New Class of Near-Infrared-Emitting Conjugated Polymers: Borylated PF8-BT

Contact Info

Product

Resources

About