Red‐Cell Metabolism in Non‐Spherocytic Congenital Haemolytic Anaemia

Effect of altitude on oxygen binding by hemoglobin and on organic phosphate levels

“…The range of values for 2,3-DPG and ATP in 12 normal subjects residing in Seattle was for 2,3-DPG, 186-309 ,AgP/ml packed cells (mean 229, SD 34) ; and for ATP, 71-100 ,ugP/ml packed cells (mean 80, SD 7.6). These values are similar to those reported by Robinson et al (8).…”

“…Likewise, blood transfused after storage shows a return to normal of both oxygen dissociation (2) and organic phosphate (4) in vivo. In anemic states oxygen dissociation curves shift to the right (5-7) and in at least one type of anemia the organic phosphate has been reported as increased (8).…”

“…The white cells were not removed with the supernatant since it was established that white cells contain only about 3% of the 2,3-DPG in normal blood. The acid-soluble phosphates in packed cells were extracted and separated according to the method of Robinson, Loder, and deGruchy (8). The method was modified so that four fractions were collected.…”

Regulatory mechanisms of hemoglobin oxygen affinity in acidosis and alkalosis

“…used by Severinghaus (6). Red cell content of the organic phosphates was obtained by extraction and fractionation using a modification (7) of the method of Robinson, Loder, and de Gruchy (8). Total phosphate in each fraction was measured using the method of Bartlett (9).…”

Clinical features and biochemical aspects of red blood cells in Hb Hirosaki.

“…Reduced glutathione (GSH) content and GSH stability were determined according to Beutler et al (1963). Adenine nucleotides and 2,3-diphosphoglycerate in erythrocytes were fractionated by column-chromatography using Dowex 1X-8 chloride resin according to Robinson et al (1961). For the analysis of inorganic phosphate the method of Takahashi (1955) was used.…”