Red blood cells are damaged by intraoperative blood salvage via Ca2+-dependent and -independent mechanisms

Liao, Xinyi; Du, Kang; Zhang, Jie; Meng, Wen-Tong; Zuo, Shanshan; Huang, Qin; Wang, Haiying; Gou, Da-Ming

doi:10.1016/j.lfs.2019.03.036

Cited by 3 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We examined this question in the present study by focusing on two processes known to affect red blood cells after transfusion during bypass surgery: phagocytosis by neutrophils and macrophages, which recognize phosphatidylserine on the surface of red blood cells [6,7]; and apoptosis, a suicide pathway in which lack of ATP, excessive oxidation and/or the presence of pro-inflammatory factors in the environment activate cysteine-containing aspartate-specific protease (caspase) 3 [8][9][10]. We explored these processes by creating cardiopulmonary bypass in a rat model that involves systemic inflammation similar to that in bypass patients [11].…”

Section: Introductionmentioning

confidence: 99%

Cardiopulmonary bypass in a rat model may shorten the lifespan of stored red blood cells by activating caspase-3

Han,

Li,

Linghu

et al. 2023

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Background Red blood cell transfusion is required for many types of surgery against cardiovascular disease, and the function of transfused cells appears to decline over time. The present study examined whether transfusion also reduces red blood cell lifespan in a rat model. Material and methods Bypass in rats were established by connecting a roll pump to the femoral artery and vein. Then FITC-labeled stored red blood cells from rats were transfused in the animals, and the cells in circulation were counted after transfusion. In separate experiments, stored red blood cells were incubated with bypass plasma in vitro, and the effects of incubation were assessed on cell morphology, redox activity, ATP level, caspase-3 activity, and phosphatidylserine exposure on the cell surface. These in vivo and in vitro experiments were also performed after pretreating the stored red blood cells with the caspase-3 inhibitor Z-DEVD-FMK. Results Bypass significantly decreased the number of circulating FITC-labeled stored red blood cells and increased the proportions of monocytes, neutrophils and splenic macrophages that had phagocytosed the red blood cells. In vitro, bypass plasma altered the morphology of red blood cells and increased oxidative stress, caspase-3 activity and phosphatidylserine exposure, while decreasing ATP level. Pretreating stored red blood cells with Z-DEVD-FMK attenuated the effects of bypass on caspase-3 activity, but not oxidative stress, in stored red blood cells. Discussion Bypass appears to shorten the lifespan of stored red blood cells, at least in part by activating caspase-3 in the cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Cardiopulmonary bypass in a rat model may shorten the lifespan of stored red blood cells by activating caspase-3

Han,

Li,

Linghu

et al. 2023

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

Non-oxidative band-3 clustering agents cause the externalization of phosphatidylserine on erythrocyte surfaces by a calcium-independent mechanism

Koshkaryev

Livshits

Pajić‐Lijaković

et al. 2020

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

Double-Facet Effect of Artificial Mechanical Stress on Red Blood Cell Deformability: Implications for Blood Salvage

et al. 2022

View full text Add to dashboard Cite

The use of intra-operative blood salvage, dialysis, and artificial organs are associated with the application of non-physiological mechanical stress on red blood cells (RBCs). To explore the effect of these procedures on red cell deformability, we determined it before and after the mechanical stress application both in an in vitro system and following a blood-saving procedure. RBC from eight healthy donors and fifteen packed RBC units were subjected to mechanical stress. RBCs from five patients undergoing orthopedic surgery were also collected. We measured the percent of undeformable cells (%UDFC) in the red cell samples using our cell flow properties image analyzer, which provides the distribution of RBC deformability in a large cell population. Mechanical stress systematically reduced the cell deformability and increased the %UDFC, while simultaneously causing hemolysis of rigid, undeformable RBCs. Ultimately, the overall result depended on the initial level of the undeformable cells; the stress-induced change in the proportion of rigid cells (Δ%UDFC) increased (Δ%UDFC > 0) when its initial value was low, and decreased (Δ%UDFC < 0) when its initial value was high. This suggests that the final impact of mechanical stress on the percent of rigid cells in the RBC population is primarily determined by their initial concentration in the sample.

show abstract

Red blood cells are damaged by intraoperative blood salvage via Ca2+-dependent and -independent mechanisms

Cited by 3 publications

References 31 publications

Cardiopulmonary bypass in a rat model may shorten the lifespan of stored red blood cells by activating caspase-3

Cardiopulmonary bypass in a rat model may shorten the lifespan of stored red blood cells by activating caspase-3

Non-oxidative band-3 clustering agents cause the externalization of phosphatidylserine on erythrocyte surfaces by a calcium-independent mechanism

Double-Facet Effect of Artificial Mechanical Stress on Red Blood Cell Deformability: Implications for Blood Salvage

Contact Info

Product

Resources

About