Red blood cells and polyelectrolyte multilayer capsules: natural carriers versus polymer-based drug delivery vehicles

Колесникова, Т. А.; Skirtach, André G.; Möhwald, Helmuth

doi:10.1517/17425247.2013.730516

Cited by 58 publications

(34 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different types of cells with diverse natures, such as erythrocytes, bacterial ghosts, genetically engineered stem and dendritic cells, have been manipulated and loaded with therapeutic agents as novel drug‐delivery systems . Among them, red blood cells (RBCs), or erythrocytes, have been extensively studied as a cell‐based drug‐delivery system because of their availability, inherent bio‐compatibility, flexibility, high in vivo stability and long systemic circulation . RBCs without nuclei can be easily loaded with different therapeutic molecules with high efficiency, or attached to various types of functional nanoparticles, for applications in drug delivery as well as biomedical imaging .…”

mentioning

confidence: 99%

Multifunctional Theranostic Red Blood Cells For Magnetic‐Field‐Enhanced in vivo Combination Therapy of Cancer

et al. 2014

View full text Add to dashboard Cite

Red blood cells are attached to iron oxide nanoparticles pre-coated with chlorine e6, a photosensitizer, and then loaded with a chemotherapeutic drug, doxorubicin, to enable imaging-guided combined photodynamic and chemotherapy of cancer, achieving excellent synergistic therapeutic effects in an animal tumor model. This work highlights the great promise of integrating cell-based drug-delivery systems with nanotechnology as a biocompatible multifunctional platform for applications in cancer theranostics.

show abstract

mentioning

confidence: 99%

Multifunctional Theranostic Red Blood Cells For Magnetic‐Field‐Enhanced in vivo Combination Therapy of Cancer

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Further assembly of microcompartment capsules can be based not only on synthetic carriers, but also based on red blood cells 75, 76 from which release has also been recently demonstrated 77.…”

Section: Bright Perspectives For Multicompartment Capsules For Theranmentioning

confidence: 99%

Towards Theranostic Multicompartment Microcapsules: in-situ Diagnostics and Laser-induced Treatment

Xiong¹,

Soenen²,

Braeckmans³

et al. 2013

Theranostics

View full text Add to dashboard Cite

Paving the way towards the application of polyelectrolyte multilayer capsules in theranostics, we describe diagnostic multi-functionality and drug delivery using multicompartment polymeric capsules which represent the next generation of drug delivery carriers. Their versatility is particularly important for potential applications in the area of theranostics wherein the carriers are endowed with the functionality for both diagnostics and therapy. Responsiveness towards external stimuli is attractive for providing controlled and on-demand release of encapsulated materials. An overview of external stimuli is presented with an emphasis on light as a physical stimulus which has been widely used for activation of microcapsules and release of their contents. In this article we also describe existing and new approaches to build multicompartment microcapsules as well as means available to achieve controlled and triggered release from their subcompartments, with a focus on applications in theranostics. Outlook for future directions in the area are highlighted.

show abstract

“…However, such vehicles have reduced clinical application because of limitations in production technologies [48] and experienced toxicity in vivo [22], [49]. An alternative to liposomes is the use of erythrocytes, whose efficacy as carrier system has been extensively documented in the last years [50]–[55]. Indeed, among the systems used in drug delivery, RBCs have demonstrated a high potential as drug carriers thanks to procedures inducing the opening of nanopores on their surface which allow the entry of molecules [53].…”

Section: Introductionmentioning

confidence: 99%

Macrophage Depletion by Free Bisphosphonates and Zoledronate-Loaded Red Blood Cells

et al. 2014

View full text Add to dashboard Cite

Bisphosphonates, besides being important drugs for the treatment of various bone diseases, could also be used to induce apoptosis in macrophage-like and cancer cells. However, their activity in vivo is limited by a short plasma half-life and rapid uptake within bone. Therefore, several delivery systems have been proposed to modify their pharmacokinetic profile and biodistribution. Among these, red blood cells (RBCs) represent one of the most promising biological carriers. The aim of this study was to select the best performing compound among Clodronate, Pamidronate, Ibandronate and Zoledronate in killing macrophages and to investigate RBCs as innovative carrier system to selectively target bisphosphonates to macrophages. To this end, the encapsulation of the selected bisphosphonates in autologous RBCs as well as the effect on macrophages, both in vitro and in vivo were studied. This work shows that, among the tested bisphosphonates, Zoledronate has proven to be the most active molecule. Human and murine RBCs have been successfully loaded with Zoledronate by a procedure of hypotonic dialysis and isotonic resealing, obtaining a dose-dependent drug entrapment with a maximal loading of 7.96±2.03, 6.95±3.9 and 7.0±1.89 µmoles of Zoledronate/ml of packed RBCs for human, Swiss and Balb/C murine RBCs, respectively. Engineered RBCs were able to detach human and murine macrophages in vitro, leading to a detachment of 66±8%, 67±8% and 60.5±3.5% for human, Swiss and Balb/C RBCs, respectively. The in vivo efficacy of loaded RBCs was tested in Balb/C mice administering 59 µg/mouse of RBC-encapsulated Zoledronate. By a single administration, depletion of 29.0±16.38% hepatic macrophages and of 67.84±5.48% spleen macrophages was obtained, confirming the ability of encapsulated Zoledronate to deplete macrophages in vivo. In conclusion, RBCs loaded with Zoledronate should be considered a suitable system for targeted delivery to macrophages, both in vitro and in vivo.

show abstract

Red blood cells and polyelectrolyte multilayer capsules: natural carriers versus polymer-based drug delivery vehicles

Abstract: In conclusion, properties of RBCs and polyelectrolyte multilayer capsules are reported here highlighting similarities and differences in their preparation and applications. In addition, their advantages and disadvantages are discussed.

Cited by 58 publications

References 101 publications

Multifunctional Theranostic Red Blood Cells For Magnetic‐Field‐Enhanced in vivo Combination Therapy of Cancer

Multifunctional Theranostic Red Blood Cells For Magnetic‐Field‐Enhanced in vivo Combination Therapy of Cancer

Towards Theranostic Multicompartment Microcapsules: in-situ Diagnostics and Laser-induced Treatment

Macrophage Depletion by Free Bisphosphonates and Zoledronate-Loaded Red Blood Cells

Contact Info

Product

Resources

About