Red blood cell processing for cryopreservation: from fresh blood to deglycerolization

Pallotta, Valeria; D’Amici, Gian Maria; D’Alessandro, Angelo; Rossetti, Roberto; Zolla, Lello

doi:10.1016/j.bcmd.2012.02.004

Cited by 43 publications

(67 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, it was proposed that osmotic effects of additional solutions cause certain morphological changes in erythrocytes and thus increase cellular volume (Mudge et al 2004). Increases in RDW values, similarly as observed by Pallotta et al (2012), might be associated with anisocytosis and cellular deformity due to low temperature (Sollberger et al 2002). MCH and MCHC mean values, in parallel to Hb concentration (Table 1) were compatible with those from studies carried out with human erythrocytes (Lecak et al 2004;Holovati et al 2008).…”

Section: Discussionsupporting

confidence: 78%

“…In parallel, more efficient storage techniques have been sought and new studies were carried out on the long-term frozen storage of red blood cells (RBCs) (Kim et al 2004;Kim et al 2007;Pallotta et al 2012;Sen and Khetarpal 2013). Storage lesions during RBC preservation are manifested by increased lactic acid, extracellular K + accumulation and a subsequent reduction in pH, adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) levels.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Freezing and storage of leukodepleted erythrocyte suspensions

Bala¹,

Eraslan²,

Akyazı³

et al. 2016

Vet. Med. - Czech

View full text Add to dashboard Cite

Studies on the frozen storage of human blood products have benefited veterinary transfusion medicine in recent years, but the long-term cryopreservation of canine red blood cells (RBCs) has not yet been thoroughly investigated. Further, no studies are available with respect to the frozen storage of leukocyte-depleted canine red blood cells (LD-RBCs). The objective of the current study was to investigate time-dependent effects of long-term frozen storage on leukocyte-depleted canine RBCs. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged for 5 min at 22 °C and 4200 × g in a cryogenic microcentrifuge and concentrated RBC (pRBC) suspensions were obtained. Leukocyte depletion was achieved by filtration (2.6 log 10 ). Then, the filtrated samples were prewashed three times in 0.9% NaCl solution and were allocated into three subgroups to be evaluated at three different time points (Day 0, Month 4 and Month 6). The samples for cryopreservation were subjected to glycerolisation and then stored at -80 °C for 4-and 6-month periods. At the end of this period pRBC units were thawed by manual agitation in a water bath maintained at 36-38 °C, centrifuged and then washed in a consecutive series of 12%, 1.6% and 0.9% of NaCl + 0.2 dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), supernatant haemoglobin (SupHb), sodium (Na + ) and potassium (K + ) levels, residual glycerol concentrations and haemograms of thawed and deglycerolised pRBC samples were evaluated together with those of Day 0. Sterility tests were performed on all samples for bacterial contamination. No statistically significant differences were noted except for Hct and SupHb levels. No bacterial contamination was noted in any of the samples on the basis of sterility tests. It was found that the described glycerolisation procedure could be a method of choice in the cryopreservation of leukocyte-depleted pRBCs (LD-pRBCs) since no negative effect was observed on the quality of the products and long-term frozen storage did not cause RBC destruction.

show abstract

Section: Discussionsupporting

confidence: 78%

mentioning

confidence: 99%

Freezing and storage of leukodepleted erythrocyte suspensions

Bala¹,

Eraslan²,

Akyazı³

et al. 2016

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…It was suggested that RBCs underwent morphological changes, probably due to the freezing process on the basis of the direct correlation between Hct and MCV levels between the groups, which might be associated with the increase in cellular volume due to the osmotic effects of the supplementary solutions. (Mudge et al 2004) Increases in RDW levels, similar to the findings of Pallota et al (2012), might be considered as an indicator of anisocytosis and cellular deformities at low temperatures (Sollberger et al 2002).…”

Section: Discussionmentioning

confidence: 84%

“…The next step was to improve blood storage techniques in an attempt to compensate growing demands for blood transfusion, and therefore, new studies were designed investigating long-term cryopreservation of RBCs (Kim et al 2004, Kim et al 2007, Pallota et al 2012. The aim was to prolong the shelf life of blood products (Pallota et al 2012) and prevent economic losses.…”

mentioning

confidence: 99%

The effects of freezing on long-term storage of canine erythrocytes

Bala¹,

Özcan²

2016

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

Human medicine studies have so far demonstrated that erythrocytes may be preserved and stored at low temperatures for decades retaining their metabolic and biochemical properties. However, detailed studies regarding this problem are not yet available in veterinary medicine. Therefore, the objective of the current study was to investigate time-dependent effects of long-term frozen storage of canine red blood cells.Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged by a cryogenic microcentrifuge and packed RBC suspensions were obtained. The samples were prewashed three times in 0.9% NaCl solution and were allocated into three groups to be evaluated at three different time points (day 0 and month 4 and 6). The samples to be frozen were subjected to glycerolization and then stored at -80 o C for 4 and 6-month periods. At the end of this period the packed RBC samples were thawed, centrifuged and then washed in a consecutive series of dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), Adenosin triphosphate (ATP), supernatant hemoglobin (SupHb), sodium (Na + ) and potassium (K + ) levels, residual glycerol concentrations and hemograms were evaluated and compared. Sterility tests were performed on all samples for bacterial contamination. A statistically significant decrease was noted in potassium levels, which was the natural outcome of deglycerolization process. No significant change was observed in terms of other parameters due based on different time points. In conclusion, long-term frozen storage had no negative effect on the quality parameters of canine erythrocytes.

show abstract

“…7,15 In general, percentages of RBCs recovered after freezing should be > 80% after any washing or cryoprotectant-removal process, and degree of RBC stability in saline solution during a 30-minute period should be > 85%. 16 The reagents 7-AAD and annexin V are used to identify cellular death and apoptosis, respectively, 17,18 and are therefore also used to assess cell viability following cryopreservation.…”

Section: Conclusion and Clinical Relevancementioning

confidence: 99%