Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)

Pavlović, Suzana; Vinterhalter, Branka; Zdravković-Korać, Snežana; Vinterhalter, D.; Zdravković, Jasmina; Cvikić, D.; Mitić, Nevena

doi:10.1007/s11240-012-0279-6

Cited by 25 publications

(26 citation statements)

References 38 publications

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“…Capitata and Brassica oleracea var. Botrytis (Pavlović et al 2013). Moreover, in most of the mentioned works the addition of PGRs, especially 2,4-D, did not increase the embryogenic response and/or resulted in a higher number of abnormal SE.…”

Section: Discussionmentioning

confidence: 92%

“…Although PGRs have been considered important for somatic embryogenesis induction in most species (Silva et al 2009;Pavlović et al 2013;Rosa et al 2015), the in vitro development of SE from cultured tissues has also been reported in the absence of PGRs (Choi et al 1998;Ć osić et al 2013). Endogenous hormone levels may be the major factors involved in determining the embryogenic competence of an explant.…”

Section: Discussionmentioning

confidence: 99%

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Embryogenic potential of immature zygotic embryos of Passiflora: a new advance for in vitro propagation without plant growth regulators

Ferreira

Sattler

Carvalho

et al. 2015

Plant Cell Tiss Organ Cult

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In vitro strategies for Passiflora have been developed owing to its economic and ecological importance. However, plantlet regeneration through somatic embryogenesis has presented some problems, such as the reproducibility of the protocol and formation of abnormal embryos and plantlets. Thus, this study aimed to establish a protocol exploring the embryogenic potential of immature zygotic embryos (IZE) of the wild species Passiflora miniata Vanderpl. and Passiflora speciosa Gardn. Friable calli, which formed on the abaxial surface of the cotyledons, yielded globular, heart-shaped, torpedo and cotyledonary somatic embryos, characterising the embryogenic response as asynchronous. A high percentage of normal regenerants (90 %) was obtained from IZE in media lacking 2,4-dichlorophenoxyacetic acid (2,4-D) in comparison to the value of normal plantlets (60 %) regenerated from mature zygotic embryos inoculated in media with 2,4-D. This result demonstrates that IZE of P. miniata and P. speciosa possess sufficient levels of endogenous phytohormones to trigger a high rate of indirect somatic embryogenesis. All regenerated plantlets had the same genome size and chromosome number as the explant donor plants. Therefore, the indirect embryogenic pathway, employing IZE inoculated into media free of growth regulators, did not cause changes in the karyotype and morphology. Based on these results, IZE should be considered as explant for the establishment of somatic embryogenesis in other species. Besides, a new, reliable and relatively rapid protocol to recover plantlets of P. miniata and P. speciosa yielded several plants, which were acclimatised and used for ornamental purposes and breeding programs, and for reintroduction into biological reserves.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Embryogenic potential of immature zygotic embryos of Passiflora: a new advance for in vitro propagation without plant growth regulators

Ferreira

Sattler

Carvalho

et al. 2015

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…In grapevine (Vitis vinifera L.) cultivars, the maximum rates of induction and germination were 99.5% and 68%, respectively [28]. Pavlović [29] reported that the highest frequencies of embryogenic calli induction in cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleraceavar.…”

Section: Resultsmentioning

confidence: 99%

High embryogenic ability and regeneration from floral axis of Amorphophallus konjac (Araceae)

et al. 2017

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Amorphophallus konjac (Araceae) a perennial herb, it has high medicinal and industrial value. In this study, a simple and efficient system for direct somatic embryogenesis and plantlet regeneration of Amorphophallus konjac was developed. The floral axis was used as the experimental material. The primary callus, developed from the floral axis grown on Murashige and Skoog (MS) medium supplemented with different hormone combination at different concentrations. The highest rate of embryogenic callus formation was observed on the MS medium containing 9.04 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 5.37 µM naphthalene acetic acid (NAA). The maximum induction rate was 79.8%, and the embryogenic calli were able to subculture on a medium containing similar hormone combination for over 1 year. The calli were also placed on different media for regeneration and it produced complete plants with shoots and root systems simultaneously. The highest differentiation rate of the embryogenic calli grown on differentiation medium supplemented with 8.88 µM 6-benzylaminopurine (6-BA) and 5.37 µM NAA was 95.6%. Flow cytometry analysis showed no ploidy variation in all the regenerate plantlets.

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“…However, medium supplementation with exogenous PGRs has a significant impact on callus inductions, morphogenesis and rooting. A lot of publications indicated that addition of cytokinins alone or with auxins at low concentration significantly increased the efficiency of cabbage regeneration (Krzyżanowska et al 2006; Pavlovic et al 2013; Gambhir and Srivastava 2015; Gerszberg et al 2015; Ertac and Tuncer 2016). At present, researchers have a range of exogenous PGRs to be used in plant in vitro culture.…”

Section: Tissue Culture Of Brassica Oleracea Var Capitatamentioning

confidence: 99%

Tissue culture and genetic transformation of cabbage (Brassica oleracea var. capitata): an overview

Gerszberg

2018

Planta

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Main conclusionThe main goal of this publication is an overview of the biotechnological achievements concerning in vitro cultures and transformation of Brassica oleracea var. capitata.Faced with the requirements of the global food market, intensified work on the genetic transformation of economically important plants is carried out in laboratories around the world. The development of efficient procedures for their regeneration and transformation could be a good solution for obtaining, in a shorter time than by traditional methods, plants with desirable traits. Furthermore, conventional breeding methods are insufficient for crop genetic improvement not only because of being time-consuming but also because they are severely limited by sexual incompatibility barriers. This problem has been overcome by genetic engineering, which seems to be a very good technique for cabbage improvement. Despite the huge progress that has been made in the field of plant regeneration and transformation methods, up to now, no routine transformation procedure has been developed in the case of cabbage. This problem stems from the fact that the efficiency of cabbage transformation is closely related to the genotype and some varieties are recalcitrant to transformation. It is obvious that it is not possible to establish one universal regeneration and transformation protocol for all varieties of cabbage. Therefore, it seems fully justified to develop the above-mentioned procedures for individual economically important cultivars. Despite the obstacles of cabbage transformation in laboratories of many countries, especially those where this vegetable is extremely popular (e.g., China, India, Korea, Malaysia, Pakistan), such attempts are made. This article reviews the achievements in the field of tissue culture and cabbage transformation from the last two decades.

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Recurrent somatic embryogenesis and plant regeneration from immature zygotic embryos of cabbage (Brassica oleracea var. capitata) and cauliflower (Brassica oleracea var. botrytis)

Cited by 25 publications

References 38 publications

Embryogenic potential of immature zygotic embryos of Passiflora: a new advance for in vitro propagation without plant growth regulators

Embryogenic potential of immature zygotic embryos of Passiflora: a new advance for in vitro propagation without plant growth regulators

High embryogenic ability and regeneration from floral axis of Amorphophallus konjac (Araceae)

Tissue culture and genetic transformation of cabbage (Brassica oleracea var. capitata): an overview

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