High embryogenic ability and regeneration from floral axis of Amorphophallus konjac (Araceae)

Abstract

Amorphophallus konjac (Araceae) a perennial herb, it has high medicinal and industrial value. In this study, a simple and efficient system for direct somatic embryogenesis and plantlet regeneration of Amorphophallus konjac was developed. The floral axis was used as the experimental material. The primary callus, developed from the floral axis grown on Murashige and Skoog (MS) medium supplemented with different hormone combination at different concentrations. The highest rate of embryogenic callus formation was observed on the MS medium containing 9.04 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 5.37 µM naphthalene acetic acid (NAA). The maximum induction rate was 79.8%, and the embryogenic calli were able to subculture on a medium containing similar hormone combination for over 1 year. The calli were also placed on different media for regeneration and it produced complete plants with shoots and root systems simultaneously. The highest differentiation rate of the embryogenic calli grown on differentiation medium supplemented with 8.88 µM 6-benzylaminopurine (6-BA) and 5.37 µM NAA was 95.6%. Flow cytometry analysis showed no ploidy variation in all the regenerate plantlets.

Multiple Methods Synergistically Promote the Synchronization of Somatic Embryogenesis Through Suspension Culture in the New Hybrid Between Pinus elliottii and Pinus caribaea

“…Somatic embryogenesis (SE) is a useful system for the mass propagation of plant material in vitro ( Gupta and Timmis, 2005 ; Kaur and Kapoor, 2016 ; Zhong et al, 2017 ) and is a morphogenetic process by which somatic cells go through a series of morphological and biochemical changes that result in the formation of a somatic or non-zygotic embryo capable of regenerating plants ( Aguilar-Hernández and Loyola-Vargas, 2018 ; Garcia et al, 2019 ). Therefore, SE has become a good system for theoretical studies in plant development, especially the molecular processes of embryo development, genetic transformation, endangered species conservation, and artificial seed production ( Quiroz-Figueroa et al, 2006 ; Aquea and Arce-Johnson, 2008 ; Hernández et al, 2011 ; Morel et al, 2014 ; Kaur and Kapoor, 2016 ; Ahn et al, 2019 ).…”

Comparative analysis of complete chloroplast genome sequences of four major Amorphophallus species

“…In general, the Amorphophallus genus produces starch and glucomannan, depending on the species. Much research has focused on in vitro propagation systems, due to the accumulation of pathogens from normal asexual reproduction, to increase the yield of Amorphophallus 3,4 . The nucleotide sequences ( ITS1 ) and plastid sequences ( rbcL and matK ) revealed a new subgeneric delineation by large-scale phylogenetic analysis of Amorphophallus 5 .…”

Enhanced Secondary Somatic Embryogenesis in Suspension Culture of Four Diploid Banana Cultivars from Kerala

“…Somatic embryos are used for studying the regulation of embryo development, but also as a tool for large-scale vegetative propagation. In some cases, somatic embryogenesis is favored over other methods of vegetative propagation because of the possibility to scale up the propagation by using bioreactors (Zhong et al, 2017). The embryogenic-callus-induced rate of the immature male florescence cultured on the media containing the recombinant protein Arg9-NLSWIND1 was significantly higher than that of the immature male florescence cultured on the control media.…”