Recovery Rate in the Concentration of Semivolatile Polycyclic Aromatic Hydrocarbon (PAH) Solutions

Mineki, Shigeru; Kawakami, Yukiko; Nakajima, Daisuke; Shiozaki, Takuya; Sugita, Kazutoshi; Shiraishi, Fujio; Takagi, Yukihiko; Goto, Sumio

doi:10.5985/jec.18.43

Cited by 3 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been determined that the recovery rate of PAHs was almost 100%. 23 All samples extracted from PUF were negative for transformed foci (data not shown). The data suggest that the gaseous matter contained few tumor promoting agents.…”

Section: Transformation Frequency At the Tumor Promotion Stage Of Ext...mentioning

confidence: 90%

Characteristics of the transformation frequency at the tumor promotion stage of airborne particulate and gaseous matter at ten sites in Japan

Ohmori

Sato

Nakajima

et al. 2013

Environ. Sci.: Processes Impacts

Self Cite

View full text Add to dashboard Cite

We used a high-volume air sampler in the summer of 2007 and the winter of 2008 at ten Japanese sites (Sapporo, Sendai, Maebashi, Tsukuba, Shinjuku, Sagamihara, Shizuoka, Touhaku, Kitakyushu, and Kagoshima) to collect total suspended particulate (TSP) and gaseous matter for evaluation. We evaluated the transformation frequency at the tumor promotion stage of these samples in a cell transformation assay using Bhas 42 cells, which were established from BALB/c 3T3 cells transfected with the v-Ha-ras oncogene. All samples collected from the gaseous matter were negative for transformed foci. There were several patterns of transformation frequency at the tumor promotion stage by area for the TSP samples. At Sapporo, the transformation frequency at the tumor promotion stage was remarkably higher in winter than in summer as well as in winter at the other sites. At six urban cities from Sendai to Shizuoka, the levels of transformed frequencies per μg of suspended particulates in winter were almost the same, and were higher than those of the remaining three sites. At three sites, Touhaku, Kitakyushu and Kagoshima, the transformation results in winter were judged as negative. The characteristics of the transformed frequencies of the compounds adsorbed on particulate matter at the sampling sites were significant in winter. We also studied the correlation between the transformation frequency at the tumor promotion stage of the TSP samples and the results of quantitative analysis of 16 polyaromatic hydrocarbons (PAHs) at the ten sites. We found that the transformation frequency at the tumor promotion stage of airborne samples could not be predicted based on the quantitative results of the PAHs in those samples. These data suggest that direct risk assessment of air samples with a bioassay is more valuable than quantitative analysis of compounds such as PAHs for predicting carcinogenicity.

show abstract

Section: Transformation Frequency At the Tumor Promotion Stage Of Ext...mentioning

confidence: 90%

Characteristics of the transformation frequency at the tumor promotion stage of airborne particulate and gaseous matter at ten sites in Japan

Ohmori

Sato

Nakajima

et al. 2013

Environ. Sci.: Processes Impacts

Self Cite

View full text Add to dashboard Cite

show abstract

“…To prepare samples for gas chromatography–mass spectrometry, 200 μg of extract was re-dissolved in 30 mL of hexane, and then 20 ng of deuterated PAHs (ES-2528, Cambridge Isotope Laboratories, Inc., Tewksbury, MA, USA) was added as an internal standard. The re-dissolved extract was again concentrated by using a rotary evaporator set at a pressure of 23,940 Pa [ 28 ]. A small amount of the concentrated hexane solution was then added to a preconditioned silica gel cartridge (Sep-Pak Silica Plus, Waters Corp. Milford, MA, USA) and eluted with 6 mL of dichloromethane–hexane (8:2 v /v).…”

Section: Methodsmentioning

confidence: 99%

Change over time of the mutagenicity in the lungs of gpt delta transgenic mice by extract of airborne particles collected from ambient air in the Tokyo metropolitan area

et al. 2018

Self Cite

View full text Add to dashboard Cite

BackgroundPreviously we found that DNA adducts were accumulated in the lungs of the rats exposed to ambient air in the Tokyo metropolitan area. To examine chronological change in in vivo mutagenicity of airborne particles, extracts produced from samples of total suspended particulates (TSP) collected from urban air in 1980, 1990, and 2010 in the Tokyo metropolitan area were intratracheally administered into the lungs of gpt delta mice, and differences in mutation and mutant frequency were determined by using the gpt assay. In vivo mutations induced by the extracts were characterized and mutation hotspots were identified by DNA sequencing of the mutated gpt gene.ResultsAdministration of the 1990 extract at a dose of 0.3 mg/animal significantly elevated total mutant frequency to 3.3-times that in vehicle control, and the in vivo mutagenicity of the extract (induced mutation frequency per milligram extract) was estimated to be 2.0- and 2.4-times higher than that of the 2010 and 1980 extract, respectively. G-to-A transition was the most common base substitution in the vehicle control mice. However, administration of the 1990 extract increased the frequency of G-to-T transversion, which is a landmark base substitution induced by oxidative stress; furthermore, when the extract was administered at a dose of 0.15 mg, the mutant and mutation frequencies of G-to-T transversion were significantly increased to frequencies comparable with those of G-to-A transition. Similar increases in the mutant and mutation frequencies of G-to-T transversion were observed after administration of the 2010 extract. Hotspots (mutation foci identified in three or more mice) of G-to-A transition mutations at nucleotides 64 and 110 were induced by the 1980, 1990, and 2010 extracts; a hotspot of G-to-T transversions at nucleotide 406 was also induced by the 2010 extract. Previously, we showed that diesel exhaust particles or their extract, as well as 1,6-dinitropyrene, administered to mice induced these hotspots of G-to-A transitions.ConclusionsThe results of the present study suggested that mutagenesis induced by extracts produced from TSP collected in the Tokyo metropolitan area induced in vivo mutagenicity via the same mechanism underlying the induction of in vivo mutagenicity by components of diesel exhaust.Electronic supplementary materialThe online version of this article (10.1186/s41021-018-0113-4) contains supplementary material, which is available to authorized users.

show abstract

A Rapid Method, Combining Microwave-Assisted Extraction and Gas Chromatography-Mass Spectrometry with a Database, for Determining Organochlorine Pesticides and Polycyclic Aromatic Hydrocarbons in Soils and Sediments

Miyawaki

Tobiishi

Takenaka

et al. 2017

Soil and Sediment Contamination: An International Journal

View full text Add to dashboard Cite

Recovery Rate in the Concentration of Semivolatile Polycyclic Aromatic Hydrocarbon (PAH) Solutions

Cited by 3 publications

References 13 publications

Characteristics of the transformation frequency at the tumor promotion stage of airborne particulate and gaseous matter at ten sites in Japan

Characteristics of the transformation frequency at the tumor promotion stage of airborne particulate and gaseous matter at ten sites in Japan

Change over time of the mutagenicity in the lungs of gpt delta transgenic mice by extract of airborne particles collected from ambient air in the Tokyo metropolitan area

A Rapid Method, Combining Microwave-Assisted Extraction and Gas Chromatography-Mass Spectrometry with a Database, for Determining Organochlorine Pesticides and Polycyclic Aromatic Hydrocarbons in Soils and Sediments

Contact Info

Product

Resources

About