Recovery of Stem Cells from Cryopreserved Periodontal Ligament

Abstract

Human post-natal stem cells possess a great potential to be utilized in stem-cell-mediated clinical therapies and tissue engineering. It is not known whether cryopreserved human tissues contain functional post-natal stem cells. In this study, we utilized human periodontal ligament to test the hypothesis that cryopreserved human periodontal ligament contains retrievable post-natal stem cells. These cryopreserved periodontal ligament stem cells maintained normal periodontal ligament stem cell characteristics, including expression of the mesenchymal stem cell surface molecule STRO-1, single-colony-strain generation, multipotential differentiation, cementum/periodontal-ligament-like tissue regeneration, and a normal diploid karyotype. Collectively, this study provides valuable evidence demonstrating a practical approach to the preservation of solid-frozen human tissues for subsequent post-natal stem cell isolation and tissue regeneration. The present study demonstrates that human post-natal stem cells can be recovered from cryopreserved human periodontal ligament, thereby providing a practical clinical approach for the utilization of frozen tissues for stem cell isolation.

Periodontal Ligament Stem Cells

“…The results of the transplantations showed a significant improvement of periodontal diseases, suggesting that PDLSC transplantation could be a safe and promising treatment for clinical periodontitis (Feng et al, 2010). Furthermore, it has been reported that human PDLSCs recovered from cryopreserved human PDL tissue maintained normal PDLSC characteristics, including expression of STRO-1, single-colony-strain generation, multipotency and cementum/PDL-like tissue generation, providing a possible and practical clinical approach for the utilization of PDLSCs isolated from frozen PDL tissues (Seo et al, 2005). These findings demonstrate that PDLSCs not only possess stem cell characteristics, including self-renewal, multipotency and immunomodulatory properties similar to BMMSCs, but also hold a unique potential to fabricate three-dimensional PDL tissue.…”

Transplantation of Cryopreserved Teeth: A Systematic Review

“…The enlargement of epithelial rests of Malassez in the cryopreserved teeth after transplantation in rat abdomen was shown to be the result of inflammation and cryoprotectant (Kawasaki et al, 2004). Seo et al showed the normal histological structure of periodontal ligament after the cryopreservation in liquid nitrogen for three and six months (Seo et al, 2005). No positive terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was observed (Seo et al, 2005).…”

“…Seo et al showed the normal histological structure of periodontal ligament after the cryopreservation in liquid nitrogen for three and six months (Seo et al, 2005). No positive terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was observed (Seo et al, 2005).…”

“…The periodontal ligament stem cell can be isolated from cryopreserved periodontal ligament (Seo et al, 2005). These cells maintained their characteristics, i.e., expression of surface marker, single colony strain generation, osteogenic/cementogenic and adipocytes differentiation, and cementun/periodontal ligament-like tissue regeneration upon subcutaneous implantation in immunocompromised mice (Seo et al, 2005).…”

Multipotent Dental Stem Cells: An Alternative Adult Derived Stem Cell Source for Regenerative Medicine

“…The potential use of dental stem cells has become even more viable for tissue regeneration and other therapies with the recent advances in cryopreservation. These advances allow proliferation and long term storage of these cells for future cell therapy treatments while maintaining their differentiation potential (Ding et al, 2010;Papaccio et al, 2006;Seo et al, 2005;Woods et al, 2009;Zhang et al, 2006).…”