Recovery of PPLO of Atypical Pneumonia on Artificial Agar Medium.

Chanock, Robert M.; Mufson, Maurice A.; James, Walter D.; Fox, H.; Bloom, Henry H.; Forsyth, Ben R.

doi:10.3181/00379727-110-27575

Cited by 48 publications

(13 citation statements)

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“…Amplification was performed in a programme of 30 cycles of 15 s at 95"C, 1 min at 55"C, 1.5 min at 72°C and a final step of 10 min at 72°C. [23] and in SP4 broth [24] at 36°C in a humidified atmosphere of C02 5% in air until colour change occurred (after 6 and 7 days for strains MAC and PI-1428, respectively). To determine bacterial density, 1 0-fold serial dilutions of the mycoplasma suspensions were made in Chanock broth and SP4 broth, respectively, and 100-pl samples were plated on modified Chanock agar and SP4 agar in triplicate.…”

Section: Ac Template For Nested Pcrmentioning

confidence: 99%

Demonstration by a nested PCR for Mycoplasma pneumoniae that M. pneumoniae load in the throat is higher in patients hospitalised for M. pneumoniae infection than in non-hospitalised subjects

Dorigo-Zetsma¹,

Zaat²,

Vriesema³

et al. 1999

Journal of Medical Microbiology

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A nested PCR protocol to detect Mycoplasma pneumoniae DNA in throat specimens was developed. An amplification control (AC) template, which is amplified by the same primers as the M. pneumoniae target sequence, was constructed. The assay allowed highly specific and sensitive detection of M. pneumoniae DNA. In all, 305 throat samples, 62 from hospitalised patients and 243 from non-hospitalised subjects, were analysed by the nested PCR. Inhibition of the PCR was observed in 20% of the samples, but was abolished after a 1 in 10 dilution. Throat samples from 5 (8%) of the hospitalised patients and from 7 (3%) of the non-hospitalised subjects were positive for M. pneumoniae DNA. To investigate the relationship between M. pneumoniae load and the severity of disease, the M. pneumoniae load in 10 throat samples from M. pneumoniae-positive subjects was assessed semi-quantitatively by application of the nested PCR to a series of limiting dilutions of nucleic acid extracted from these throat samples. The calculated M. pneumoniae load varied from 20 to 3830cfu/ml of throat sample. The mean M. pneumoniae load in samples from the hospitalised patients was significantly higher than that in samples from the non-hospitalised subjects. The nested PCR is a useful tool to detect M. pneumoniae DNA in the throat and to study the relationship between the load of M. pneumoniae in throat samples and severity of disease due to M. pneumoniae infection.

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Section: Ac Template For Nested Pcrmentioning

confidence: 99%

Demonstration by a nested PCR for Mycoplasma pneumoniae that M. pneumoniae load in the throat is higher in patients hospitalised for M. pneumoniae infection than in non-hospitalised subjects

Dorigo-Zetsma¹,

Zaat²,

Vriesema³

et al. 1999

Journal of Medical Microbiology

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“…Liquid, semi-solid or diphasic PPL0 media were prepared according to CnA~C0CK et al (4,5) and K~n~ et al (20). Liquid, semi-solid or diphasic PPL0 media were prepared according to CnA~C0CK et al (4,5) and K~n~ et al (20).…”

Section: Mp Paccaud and CL Jacqci~rmentioning

confidence: 99%

A Respiratory syncytial virus of bovine origin

Paccaud¹,

Jacquier²

1970

Archiv f Virusforschung

128

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An outbreak of respiratory disease which spread to all cattle aged less than 7 years in two farms, in the village of Avusy (canton of Geneva), is briefly described. The course of the disease was more severe in older animals, which showed frequently symptoms of bronchopneumonia, than in younger ones. The disease lasted from 3 to 10 days. No deaths were reported.A virus antigenically related to the human respiratory syncytial viruses was isolated from the conjunctivo-nasal specimens of one animal in each farm. Evidence of the bovine origin of both strains was brought by the sero-conversions displayed by the cattle under study in neutralization and complement fixation tests and by some biological traits of the virus as well.

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“…Norniul Z'PLO agar was as described by Chunock et al (7), except that amphotericin was omitted and 1 per cent dextrose was added. It thus consisted of 7 parts PPLO agar "Difco" without crystal violet, 2 parts non-inactivated horse serum and 1 part 25 per cent yeast extract, and also contained 1 per cent dextrose, 0.05 pcr cent Thallium acetate and 500 units of Penicillidml.…”

Section: A T E R I a L A N D M E T H O D S Meiliumentioning

confidence: 99%

Studies on the Complement Fixation Test With Mycoplasma Pneumoniae Antigen

Eng¹

1967

Acta Pathologica Microbiologica Scandinavica

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Recovery of PPLO of Atypical Pneumonia on Artificial Agar Medium.

Cited by 48 publications

References 0 publications

Demonstration by a nested PCR for Mycoplasma pneumoniae that M. pneumoniae load in the throat is higher in patients hospitalised for M. pneumoniae infection than in non-hospitalised subjects

Demonstration by a nested PCR for Mycoplasma pneumoniae that M. pneumoniae load in the throat is higher in patients hospitalised for M. pneumoniae infection than in non-hospitalised subjects

A Respiratory syncytial virus of bovine origin

Studies on the Complement Fixation Test With Mycoplasma Pneumoniae Antigen

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