A Respiratory syncytial virus of bovine origin

Paccaud, M. F.; Jacquier, Cl.

doi:10.1007/bf01258363

Cited by 128 publications

(91 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4,9,19 Extensive antigen cross-reactivity exists between human and cattle strains of RSV. 12,16,18,20 T h e objective of this study was to determine the diagnostic accuracy of RSV EIA in detecting BRSV by comparison with other methods used in our laboratory.…”

mentioning

confidence: 99%

Detection of Bovine Respiratory Syncytial Virus using a Heterologous Antigen-Capture Enzyme Immunoassay

et al. 1989

View full text Add to dashboard Cite

Abstract. Based on the marked antigenic similarities that exist between antigens of the human and bovine strains of respiratory syncytial virus (RSV), an enzyme immunoassay (EIA) designed to detect human RSV was used to detect bovine RSV. The commercial test kit (RSV EIA) consists of a solid phase (beads) coated with a capture antiserum prepared against the Long strain of human RSV.The RSV EIA test was compared with the method of inoculation of cell cultures and fluorescent antibody (FA) staining of lung tissue for the detection of bovine RSV. Using a cell culture-propagated stock of strain 375 of bovine RSV, the threshold of sensitivity of the EIA test for the cattle strain of RSV was determined to be ≤ 10 2,3 CCID 50 /ml. In addition, RSV EIA detected the bovine RSV in nasal samples obtained from 3 experimentally inoculated cattle. The RSV EIA exhibited a sensitivity of ≥ 80% during the period that shedding of infectious virus took place.All of the bovine RSV FA-positive lung samples (n = 37) were positive by the RSV EIA. Twenty-six of the remaining 214 bovine RSV FA-negative lung samples were positive by the RSV EIA. The RSV EIA was also used to test 137 nasal swabs obtained from cases of bovine respiratory disease. Of these, 38 tested positive by RSV EIA. All samples that tested positive by EIA were confirmed by blocking assays using hyperimmune serum anti-bovine RSV and a pool of monoclonal antibodies specific for that virus.

show abstract

mentioning

confidence: 99%

Detection of Bovine Respiratory Syncytial Virus using a Heterologous Antigen-Capture Enzyme Immunoassay

et al. 1989

View full text Add to dashboard Cite

show abstract

“…In 1970 Paccaud and Jacquier published a report of an outbreak of bovine respiratory disease in Swiss cattle (55 Serological studies were conducted with acute and convalescent serum samples of infected cattle. There were sero-conversions in both affected herds, including the animals from which the viruses were isolated.…”

Section: It Was In This Context That the Search For New Viral Agentsmentioning

confidence: 99%

“…In order to designate the new isolate, FSl-1, as a bovine respiratory syncytial virus, it must show a close antigenic relation ship to the human RS virus and yet be unquestionably of bovine origin (33,55). Cross-neutralization tests with rabbit antiserum to both the bovine and human isolates as summarized in Table 2 indicate the antigenic similarity between the two viruses.…”

Section: Or-'mentioning

confidence: 99%

Isolation, characterization, and pathogenicity studies of a bovine respiratory syncytial virus

Smith

Frey

Dierks

1975

Archives of Virology

View full text Add to dashboard Cite

“…Diversas referências a infecções associadas a vírus (BVD, parainfluenza-3 e adenovirus bovino) e a bactérias (Pasteurella sp, Streptococcus sp, Mycoplasma sp e Pseudomonas sp (Inaba et al 1970, Lehmkuhl et al 1979, Pirie 1981) demonstram a importância do BRSV como patógeno respiratório. Em bezerros, o BRSV foi identificado desde 1967; seu primeiro isolamento foi feito na Suécia por Paccaud & Jacquier (1970). No Brasil, o primeiro registro da presença do vírus foi feita por Gonçalves et al (1993), através de imunoflorescência e cultivo celular de amostras de tecido pulmonar de bezerros, coletadas em frigoríficos na Grande Porto Alegre, RS.…”

unclassified

Infecção natural pelo Vírus Sincicial Respiratório Bovino (BRSV) no Estado de Alagoas

et al. 2000

View full text Add to dashboard Cite

RESUMO.-Descreve-se a ocorrência de infecção pelo vírus sincicial respiratório bovino (BRSV) em bezerros descendentes de animais das raças pardo-suíça e holandesa importados da Alemanha, Áustria, Suíça e Uruguai, na qual morreram em Alagoas, Brasil, pelo menos 220 cabeças, de 1995 até a presente data. O quadro clínico caracterizou-se por hipertermia, tosse seca, mais tarde dispnéia acentuada e por vezes lacrimejamento; à auscultação havia estertores secos, depois úmi-dos, com sibilos, muitas vezes audíveis à distância. O exame histológico revelou pneumonia intersticial com formação de células sinciciais, infiltração predominantemente linfocitária com presença de eosinófilos e de corpúsculos de Russel, proliferação de pneumócitos tipo II e leve metaplasia escamosa. Células epiteliais de bronquíolos e células sinciciais marcaram-se positivamente com o anticorpo anti-BRSV. A ocorrên-cia da enfermidade no Sul e agora no Nordeste do Brasil indica a necessidade de se promover um amplo levantamento epidemiológico para se avaliar o grau de perdas e a proporção de animais infectados no país. Lembramos que parte dos animais importados, ao que tudo indica, já estavam infectados nos países de origem, quando desembarcaram em Belém, Pará. Cases of bovine respiratory syncytial virus (BRSV) infection affecting calves in the State of Alagoas, Brazil, are described. At least 220 calves, which were the progeny of Brown Swiss and Holstein Friesian cattle imported from Germany, Austria, and Uruguay, have died from the disease since 1995. Clinical signs included fever, dry cough, serous ocular discharge and, towards the final stages, marked dyspnea. On auscultation there were loud and harsh breathing

show abstract

A Respiratory syncytial virus of bovine origin

Cited by 128 publications

References 22 publications

Detection of Bovine Respiratory Syncytial Virus using a Heterologous Antigen-Capture Enzyme Immunoassay

Detection of Bovine Respiratory Syncytial Virus using a Heterologous Antigen-Capture Enzyme Immunoassay

Isolation, characterization, and pathogenicity studies of a bovine respiratory syncytial virus

Infecção natural pelo Vírus Sincicial Respiratório Bovino (BRSV) no Estado de Alagoas

Contact Info

Product

Resources

About