Recovery of known‐age <i>Hyalella azteca</i> (amphipoda) from sediment toxicity tests

Tomasovic, Michael J.; Dwyer, F. James; Greer, I. Eugene; Ingersoll, Christopher G.

doi:10.1002/etc.5620140708

Cited by 19 publications

(1 citation statement)

References 5 publications

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“…Some of the variation in reproduction rates among treatments may reflect differential mortality of neonate amphipods. Neonates would be exposed to Cr-(VI) for up to 7 d between sampling dates (15) and dead neonates would not be visible after only a few days due to rapid decomposition (23). Overall, these results indicate that reduced survival of H. azteca is a sensitive response to chronic Cr(VI) exposure.…”

Section: Resultsmentioning

confidence: 79%

Effects of Sediment Characteristics on the Toxicity of Chromium(III) and Chromium(VI) to the Amphipod, Hyalella azteca

Besser

Brumbaugh

Kemble

et al. 2004

Environ. Sci. Technol.

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We evaluated the influence of sediment characteristics, acid-volatile sulfide (AVS) and organic matter (OM), on the toxicity of chromium (Cr) in freshwater sediments. We conducted chronic (28-42-d) toxicitytests with the amphipod Hyalella azteca exposed to Cr(VI) and Cr(III) in water and in spiked sediments. Waterborne Cr(VI) caused reduced survival of amphipods with a median lethal concentration (LC50) of 40 microg/L. Cr(VI) spiked into test sediments with differing levels of AVS resulted in graded decreases in AVS and sediment OM. Only Cr(VI)-spiked sediments with low AVS concentrations (< 1micromol/g) caused significant amphipod mortality. Waterborne Cr(III) concentrations near solubility limits caused decreased survival of amphipods at pH 7 and pH 8 but not at pH 6. Sediments spiked with high levels of Cr(III) did not affect amphipod survival but had minor effects on growth and inconsistent effects on reproduction. Pore waters of some Cr(III)-spiked sediments contained measurable concentrations of Cr(VI), but observed toxic effects did not correspond closely to Cr concentrations in sediment or pore waters. Our results indicate that risks of Cr toxicity are low in freshwater sediments containing substantial concentrations of AVS.

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Section: Resultsmentioning

confidence: 79%

Effects of Sediment Characteristics on the Toxicity of Chromium(III) and Chromium(VI) to the Amphipod, Hyalella azteca

Besser

Brumbaugh

Kemble

et al. 2004

Environ. Sci. Technol.

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Using an interlaboratory study to revise methods for conducting 10‐d to 42‐d water or sediment toxicity tests with Hyalella azteca

Ivey

Ingersoll

Brumbaugh

et al. 2016

Enviro Toxic and Chemistry

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Studies have been conducted to refine US Environmental Protection Agency, ASTM International, and Environment Canada standard methods for conducting 42-d reproduction tests with Hyalella azteca in water or in sediment. Modifications to the H. azteca method include better-defined ionic composition requirements for exposure water (i.e., >15 mg/L of chloride and >0.02 mg/L of bromide) and improved survival, growth, and reproduction with alternate diets provided as increased rations over time in water-only or whole-sediment toxicity tests. A total of 24 laboratories volunteered to participate in the present interlaboratory study evaluating the performance of H. azteca in 42-d studies in control sand or control sediment using the refined methods. Improved growth and reproduction of H. azteca was observed with 2 alternate diets of 1) ramped diatoms (Thalassiosira weissflogii) + ramped Tetramin or 2) yeast-cerophyll-trout chow (YCT) + ramped Tetramin, especially when compared with results from the traditional diet of 1.8 mg YCT/d. Laboratories were able to meet proposed test acceptability criteria and in most cases had lower variation in growth or reproduction compared with previous interlaboratory studies using the traditional YCT diet. Laboratory success in conducting 42-d H. azteca exposures benefited from adherence to several key requirements of the detailed testing, culturing, and handling methods. Results from the present interlaboratory study are being used to help revise standard methods for conducting 10-d to 42-d water or sediment toxicity exposures with H. azteca. Environ Toxicol Chem 2016;35:2439-2447. © 2016 SETAC.

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Validation of a new standardized test method for the freshwater amphipod Hyalella azteca: Determining the chronic effects of silver in sediment

Taylor

Novak²,

Rendas³

et al. 2016

Enviro Toxic and Chemistry

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Environment Canada has developed a new 42-d sediment toxicity test method that includes a reproduction test endpoint with the freshwater amphipod Hyalella azteca. Because of concerns that existing standard methodologies, whereby adults are transferred to a water-only exposure before release of their first brood at day 28, will lead to internal contaminant depuration and loss of sensitivity, the Environment Canada methodology conducts the entire exposure in sediment. To demonstrate applicability of the method for assessing the toxicity of chemical-spiked sediment, H. azteca were exposed for 42 d to sediment amended with silver nitrate (AgNO ). Mortality was significantly higher at the highest sediment concentration of Ag (2088 mg/kg dry wt); however, there was no significant reduction in biomass or reproduction as a result of Ag exposure despite significant bioaccumulation. Based on Ag measurements and speciation modeling, the principle route of Ag exposure was likely through the ingestion of complexed colloidal or particulate Ag. The techniques used to recover young amphipods from sediment were critical, and although this effort can be labor intensive (20-45 min/replicate), the technicians demonstrated 91% recovery in blind trials. For the first time, Environment Canada will require laboratories to report their recovery proficiency for the 42-d test-without this information, data will not be accepted. Overall, the reproduction test will be more applicable when only a few chemical concentrations need to be evaluated in laboratory-amended sediments or for field-collected contaminated site assessments (i.e., contaminated site vs reference site comparisons). Environ Toxicol Chem 2016;35:2430-2438. © 2016 SETAC.

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Recovery of known‐age Hyalella azteca (amphipoda) from sediment toxicity tests

Cited by 19 publications

References 5 publications

Effects of Sediment Characteristics on the Toxicity of Chromium(III) and Chromium(VI) to the Amphipod, Hyalella azteca

Effects of Sediment Characteristics on the Toxicity of Chromium(III) and Chromium(VI) to the Amphipod, Hyalella azteca

Using an interlaboratory study to revise methods for conducting 10‐d to 42‐d water or sediment toxicity tests with Hyalella azteca

Validation of a new standardized test method for the freshwater amphipod Hyalella azteca: Determining the chronic effects of silver in sediment

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