Recovery of Cryptosporidium oocysts from small and large volume water samples using a compressed foam filter system

Sartory, D. P.; Parton, A.; Roberts, Jacqueline M.; Bergmann, K.

doi:10.1046/j.1472-765x.1998.00459.x

Cited by 21 publications

(12 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this experiment, the FM method yielded a higher recovery for both oocysts and cysts. In fact, the recovery of Giardia cysts was often higher than that of Cryptosporidium oocysts with all filters tested using distilled water, a result which is supported by the previous findings of other investigators (11,26). This result could probably be due to the larger size of the Giardia cysts, which makes them easier to capture in the filtration material (7).…”

Section: Discussionsupporting

confidence: 85%

Evaluation of Five Membrane Filtration Methods for Recovery of Cryptosporidium and Giardia Isolates from Water Samples

Wohlsen

Bates

Gray

et al. 2004

Appl Environ Microbiol

View full text Add to dashboard Cite

We evaluated the efficiency of five membrane filters for recovery of Cryptosporidium parvum oocysts and Giardia lamblia cysts. These filters included the Pall Life Sciences Envirochek (EC) standard filtration and Envirochek high-volume (EC-HV) membrane filters, the Millipore flatbed membrane filter, the Sartorius flatbed membrane filter (SMF), and the Filta-Max (FM) depth filter. Distilled and surface water samples were spiked with 10 oocysts and 10 cysts/liter. We also evaluated the recovery efficiency of the EC and EC-HV filters after a 5-s backwash postfiltration. The backwashing was not applied to the other filtration methods because of the design of the filters. Oocysts and cysts were visualized by using a fluorescent monoclonal antibody staining technique. For distilled water, the highest percent recovery for both the oocysts and cysts was obtained with the FM depth filter. However, when a 5-s backwash was applied, the EC-HV membrane filter (EC-HV-R) was superior to other filters for recovery of both oocysts (n ‫؍‬ 53 ؎ 15.4 per 10 liters) and cysts (n ‫؍‬ 59 ؎ 11.5 per 10 liters). This was followed by results of the FM depth filter (oocysts, 28.2 ؎ 8, P ‫؍‬ 0.015; cysts, 49.8 ؎ 12.2, P ‫؍‬ 0.4260), and SMF (oocysts, 16.2 ؎ 2.8, P ‫؍‬ 0.0079; cysts, 35.2 ؎ 3, P ‫؍‬ 0.0079). Similar results were obtained with surface water samples. Giardia cysts were recovered at higher rates than were Cryptosporidium oocysts with all five filters, regardless of backwashing. Although the time differences for completion of filtration process were not significantly different among the procedures, the EC-HV filtration with 5-s backwash was less labor demanding.

show abstract

Section: Discussionsupporting

confidence: 85%

Evaluation of Five Membrane Filtration Methods for Recovery of Cryptosporidium and Giardia Isolates from Water Samples

Wohlsen

Bates

Gray

et al. 2004

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…1997), ultrafiltration (Simmons et al. 2001; Kuhn and Oshima 2002), continuous separation channel centrifugation (Borchardt and Spencer 2002) and compressed foam filter (Sartory et al. 1998).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cryptosporidium parvum oocyst recovery efficiencies from various filtration cartridges by electrochemiluminescence assays

Lee

Gómez

McAuliffe

et al. 2004

Lett Appl Microbiol

View full text Add to dashboard Cite

. Various dosages of C. parvum oocysts were spiked into water samples with a wide range of turbidity (10-50 NTU). Electrochemiluminescence assays were employed to enumerate viable or total number of C. parvum oocysts in these eluates. Among the cartridges tested, Filta-Max consistently showed higher oocyst recovery efficiency, especially with large volume, highly turbid water samples. Conclusions: Filtra-Max filter is the best performer because of its higher oocyst recovery efficiency. Significance and Impact of the Study: The overall sensitivities of various C. parvum oocyst detection assays in water samples can be improved if highly efficient oocyst recovery filtration cartridges such as Filtra-Max are incorporated in sample preparation.

show abstract

“…One of the biggest problems is the lack of a consistent method to simultaneously concentrate multiple organisms from a single water sample. Another common difficulty is the broad variation in recoveries, especially from water samples with high turbidity levels (1,15,16,23). Additionally, cost is an important factor in the detection, monitoring, and identification of pathogenic microorganisms because different methods of concentration are frequently used for viruses, protozoan parasites, and bacteria.…”

mentioning

confidence: 99%

Optimization of a Reusable Hollow-Fiber Ultrafilter for Simultaneous Concentration of Enteric Bacteria, Protozoa, and Viruses from Water

Morales-Morales

Vidal

Olszewski

et al. 2003

Appl Environ Microbiol

121

102

View full text Add to dashboard Cite

The detection and identification of pathogens from water samples remain challenging due to variations in recovery rates and the cost of procedures. Ultrafiltration offers the possibility to concentrate viral, bacterial, and protozoan organisms in a single process by using size-exclusion-based filtration. In this study, two hollow-fiber ultrafilters with 50,000-molecular-weight cutoffs were evaluated to concentrate microorganisms from 2-and 10-liter water samples. When known quantities (10 5 to 10 6 CFU/liter) of two species of enteric bacteria were introduced and concentrated from 2 liters of sterile water, the addition of 0.1% Tween 80 increased Escherichia coli strain K-12 recoveries from 70 to 84% and Salmonella enterica serovar Enteritidis recoveries from 36 to 72%. An E. coli antibiotic-resistant strain, XL1-Blue, was recovered at a level (87%) similar to that for strain K-12 (96%) from 10 liters of sterile water. When E. coli XL1-Blue was introduced into 10 liters of nonsterile Rio Grande water with higher turbidity levels (23 to 29 nephelometric turbidity units) at two inoculum levels (9 ؋ 10 5 and 2.4 ؋ 10 3 per liter), the recovery efficiencies were 89 and 92%, respectively. The simultaneous addition of E. coli XL1-Blue (9 ؋ 10 5 CFU/liter), Cryptosporidium parvum oocysts (10 oocysts/liter), phage T1 (10 5 PFU/liter), and phage PP7 (10 5 PFU/liter) to 10 liters of Rio Grande surface water resulted in mean recoveries of 96, 54, 59, and 46%, respectively. Using a variety of surface waters from around the United States, we obtained recovery efficiencies for bacteria and viruses that were similar to those observed with the Rio Grande samples, but recovery of Cryptosporidium oocysts was decreased, averaging 32% (the site of collection of these samples had previously been identified as problematic for oocyst recovery). Results indicate that the use of ultrafiltration for simultaneous recovery of bacterial, viral, and protozoan pathogens from variable surface waters is ready for field deployment.

show abstract

Recovery of Cryptosporidium oocysts from small and large volume water samples using a compressed foam filter system

Cited by 21 publications

References 9 publications

Evaluation of Five Membrane Filtration Methods for Recovery of Cryptosporidium and Giardia Isolates from Water Samples

Evaluation of Five Membrane Filtration Methods for Recovery of Cryptosporidium and Giardia Isolates from Water Samples

Evaluation of Cryptosporidium parvum oocyst recovery efficiencies from various filtration cartridges by electrochemiluminescence assays

Optimization of a Reusable Hollow-Fiber Ultrafilter for Simultaneous Concentration of Enteric Bacteria, Protozoa, and Viruses from Water

Contact Info

Product

Resources

About