Recovery of contractile function of cryodamaged rat myocardium after transplantation of fetal cardiomyocytes and predifferentiated bone marrow stromal stem cells

Онищенко, Н. А.; Потапов, И. В.; Башкина, Л. В.; Крашенинников, М. Е.; Зайденов, В. А.; Avramov, Predrag

doi:10.1007/s10517-004-0011-6

Cited by 8 publications

(14 citation statements)

References 11 publications

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“…The animals were divided into 4 groups: 1) culture medium without cell material; 2) culture medium with BM mononuclear fraction (3×10 6 cell/ml); 3) MSC (2×10 5 cell/ml); and 4) medium with predifferentiated MSC (2×10 5 cell/ml). The concentrations of transplanted cells were selected on the basis of our findings and published data [5].…”

Section: Methodsmentioning

confidence: 99%

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Effect of Transplantation of Bone Marrow Cells on Morphology of Rat Myocardium after Cryodestruction

et al. 2009

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We studied the effects of bone marrow cell transplantation on myocardium of the prenecrotic zone in Wistar rats. Intramyocardial cell transplantation reduced the severity of hypertrophy of myocardium and the degree of its cicatricial degeneration on day 40 after cryodestruction. The morphology of the myocardium in the prenecrotic zone depended on the type of transplanted cells. The course of inflammation was swifter; vascularization of the myocardium was more intensive. The best effect, evaluated by the number of new vessels, was observed after MSC transplantation. Hence, the positive effect of bone marrow cell transplantation is realized at the expense of more rapid structural organization of the damaged site and stimulation of myocardial vascularization.

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Section: Methodsmentioning

confidence: 99%

“…Mesenchymal stem cells were isolated from pre-isolated MNF by the standard method [5]. Directed differentiation of MSC was induced by adding 5-azacitidine (3 µmol/liter) [6].…”

Section: Methodsmentioning

confidence: 99%

Effect of Transplantation of Bone Marrow Cells on Morphology of Rat Myocardium after Cryodestruction

et al. 2009

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“…Viability of mononuclears and total cellularity of the material were evaluated [7]. The number of viable cells was adjusted to 5×10 6 cell/ml, transferred to 50-ml plastic flasks (10 ml per flask), and cultured for 14 days [8]. The medium was then discharged and the feeder layer was treated with 0.1% trypsin and collagenase.…”

Section: Methodsmentioning

confidence: 99%

Effect of stress-proteins on survival of bone marrow mesenchymal stem cells after intramyocardial transplantation against the background of postinfarction heart remodeling

et al. 2008

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We studied the presence of colony-forming cells in cell culture from rat heart 40 days after experimental myocardial infraction. The mean cellularity in this pathology was 12+/-8 cell/cm2, which is 20-fold lower than in intact myocardium. Transplantation of mesenchymal stem cells into the remodeling myocardium restored the pool of colony-forming cells. This effect depended on the state of transplanted cells. After transplantation of mesenchymal stem cells with low content of stress proteins, 6+/-2 colonies were detected, while after transplantation of cells with high content of hsp70 and hsp60 stress proteins (modified mesenchymal stem cells) 18+/-5 colonies were found, the mean cellularity of the corresponding cultured being 946+/-267 and 1926+/-123 cell/cm2. The positive effect of modified mesenchymal stem cells was observed on days 4 and 7 after transplantation. We conclude that postinfarction remodeling mobilized the total pool of regional stem cells; mesenchymal stem cells with high content of hsp70 and hsp60 demonstrated highest survival rate after intramyocardial transplantation.

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“…The myocardium was damaged by cryodestruction [4]. The operation was carried out under light ether narcosis.…”

Section: Methodsmentioning

confidence: 99%

“…The bone marrow contains precursor blood cells and mesenchymal stem cells (MSC) capable of differentiating into other cells [14]. Factors promoting directed differentiation of MSC, including differentiation into cardiomyocytes, at the stage of ulturing were identified [4,6,8]. The efficiency of using nonfractionated bone marrow cells for transplantation is discussed [1,11].…”

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confidence: 99%

Colony-forming cells in rat myocardium after destructive exposure and intramyocardial transplantation of bone marrow cells

et al. 2008

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The content of colony-forming cells in myocardial cell culture from the perinecrotic zone of rat heart was evaluated on day 40 after cryodestruction. The mean cellularity after cryodestruction was 12-fold lower than in intact animals. Intramyocardial transplantation of bone marrow cells (mononuclears, mesenchymal stem cells, and mesenchymal stem cells treated with 5-azacitidine) into the perinecrotic zone increased the content of colony-forming cells. After transplantation of mesenchymal stem cells and mesenchymal stem cells treated with 5-azacitidine, the number of colonies reached 33 +/- 7 and 11 +/- 3, the mean cellularity being 2975 +/- 80 and 1105 +/- 42 cells/cm2, respectively. Hence, intramyocardial transplantation of mesenchymal stem cells created an appreciable pool of colony-forming cells in the myocardial perinecrotic zone. Treatment with 5-azacitidine reduced survival of mesenchymal stem cells after intramyocardial transplantation.

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Recovery of contractile function of cryodamaged rat myocardium after transplantation of fetal cardiomyocytes and predifferentiated bone marrow stromal stem cells

Cited by 8 publications

References 11 publications

Effect of Transplantation of Bone Marrow Cells on Morphology of Rat Myocardium after Cryodestruction

Effect of Transplantation of Bone Marrow Cells on Morphology of Rat Myocardium after Cryodestruction

Effect of stress-proteins on survival of bone marrow mesenchymal stem cells after intramyocardial transplantation against the background of postinfarction heart remodeling

Colony-forming cells in rat myocardium after destructive exposure and intramyocardial transplantation of bone marrow cells

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