Recovery of Biological Products in Aqueous Two Phase Systems

Abstract

Recovering of biological products, such as enzymes, proteins, nucleic acids, amino acids and microorganisms, from the contaminants requires many steps, each removal of the contaminants, isolating of target product and purification of product closer to the demand. For the success of the large scale production, there is a need for improving process economic, efficient and delicate downstream technique enough to preserve the properties of bio-products. Aqueous two phase systems (ATPSs) have overcome these demands and emerged as a powerful method for the downstream processing of bio-products. This paper is intended to encourage a starting idea for beginners in using ATPSs technique with biological products. Factors contributing to the partition behavior of biomolecules between the two phases including polymer molecular weight and concentration, biomolecule size, surface charge, pH and temperature are presented.

Application of aqueous two phase systems based on polyethylene glycol and sodium citrate for the recovery of phenolic compounds from Eucalyptus wood

“…Many researchers studied various applications of ATPS for the extraction and purification of biological products (Benavides and Rito-Palomares 2008, Aguilar and Rito-Palomares 2010, Ratanapongleka 2010. Despite many recent studies on the subject, there is still no extensive information about extraction of natural antioxidants with this technique and direct application over raw materials (Hassman et al 2008, Wang et al 2008, Wu et al 2011, Xavier et al 2013).…”

Astaxanthin production from sewage of traditional Thai rice vermicelli

“…However, this option is not very attractive because the COD of the wastewater is not high enough to be cost effective considering high investment in infrastructure and equipment. A few researchers have explored other alternatives to better utilize the wastewater including that for producing bioethanol [1,2,3]. Probably one of the better idea is to turn this wastewater into high-value products by fermentation.…”

Purification and characterizations of a novel recombinant Bacillus velezensis endoglucanase by aqueous two-phase system

“…The numbers in the brackets are the accession numbers of rpoB gene sequences from GenBank Due to the enzyme activity loss in NTA affinity column, the recombinant enzyme Egls was re-purified using PEG 4000, phosphate buffer, and NaCl aqueous two-phase system. Aqueous two-phase system is one of the most economical, high-efficient, easy-to-operate, and scale-up methods, which has been extensively used in bio-products purification, such as enzymes, proteins, nucleic acids, and amino acids (Ratanapongleka 2010). The factors, such as polymer molecular weight, pH, temperature, biomolecule size, surface charge, also contribute to the partition behavior of biomolecules between the two phases (Ratanapongleka 2010).…”

“…Aqueous two-phase system is one of the most economical, high-efficient, easy-to-operate, and scale-up methods, which has been extensively used in bio-products purification, such as enzymes, proteins, nucleic acids, and amino acids (Ratanapongleka 2010). The factors, such as polymer molecular weight, pH, temperature, biomolecule size, surface charge, also contribute to the partition behavior of biomolecules between the two phases (Ratanapongleka 2010). The Binodal curves were built to use as a guideline for the enzyme purification in ATPS (data not shown).…”