Recovery in culture of viable but nonculturable <i>Vibrio parahaemolyticus</i>: regrowth or resuscitation?

Coutard, François; Crassous, Philippe; Droguet, Mickaël; Gobin, Eric; Colwell, Rita R.; Pommepuy, Monique; Hervio-Heath, Dominique

doi:10.1038/ismej.2007.1

Cited by 57 publications

(47 citation statements)

References 35 publications

(27 reference statements)

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“…populations in artificial and natural seawater has been extensively studied, and it has been demonstrated that nutrient deprivation, elevated salinity, and/ or reduced temperature strongly induce transition to the VBNC state [5,[38][39][40]. Analysis of Vibrio responses to the environmental factors revealed some common features OM outer membrane, P periplasm, CM cytoplasmic membrane, C cytoplasmic, NCyt noncytoplasmic according to PSORTb 3.0 program including the stress-induced changes in cell physiology and morphology [15,41].…”

Section: Discussionmentioning

confidence: 99%

Changes in the Vibrio harveyi Cell Envelope Subproteome During Permanence in Cold Seawater

et al. 2016

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Previous work demonstrated that physiological, morphological, and gene expression changes as well as the time-dependent entry into the viable but not culturable (VBNC) state are used by Vibrio species to survive and cope with diverse stress conditions including seasonal temperature downshifts and starvation. To learn more about the nature and specific contribution of membrane proteins to cell adaptation and survival, we analyzed variations in the protein composition of cell envelope and related them to morphological and physiological changes that were taking place during the long-term permanence of Vibrio harveyi in seawater microcosm at 4 °C. We found that after 21 days of permanence, nearly all population (ca. 99 %) of V. harveyi acquired the VBNC phenotype. Although the size of V. harveyi cells gradually decreased during the incubation time, we found that this morphological change was not directly related to their entry into the VBNC state. Our proteomic study revealed that the level of membrane proteins playing key roles in cellular transport, maintenance of cell structure, and in bioenergetics processes remained unchanged along starvation at low temperature, thus suggesting that V. harveyi might need these proteins for the long-term survival and/or for the resuscitation process. On a contrary, the level of two proteins, elongation factor Tu (EF-TU) and bacterioferritin, greatly increased reaching the maximal values by the end of the incubation period. We further discuss the above data with respect to the putative roles likely exerted by membrane proteins during transition to and maintaining of the VBNC state.

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Section: Discussionmentioning

confidence: 99%

Changes in the Vibrio harveyi Cell Envelope Subproteome During Permanence in Cold Seawater

et al. 2016

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“…In coastal water, during winter months, when SST dips below the preferred range, most vibrios can enter a dormant state known as "viable but nonculturable" (VBNC) (54,55). Other factors, both chemical and environmental, which have been reported to induce the VBNC state, are diverse and numerous.…”

Section: Effect Of Increasing Sst On Vibrio Growth and Biology: Fieldmentioning

confidence: 99%

Effects of Global Warming onVibrioEcology

et al. 2015

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Vibrio-related infections are increasing worldwide both in humans and aquatic animals. Rise in global sea surface temperature (SST), which is approximately 1°C higher now than 140 years ago and is one of the primary physical impacts of global warming, has been linked to such increases. In this chapter, major known effects of increasing SST on the biology and ecology of vibrios are described. They include the effects on bacterial growth rate, both in the field and in laboratory, culturability, expression of pathogenicity traits, and interactions with aquatic organisms and abiotic surfaces. Special emphasis is given to the effect of ocean warming on Vibrio interactions with zooplankters, which represent one of the most important aquatic reservoirs for these bacteria. The reported findings highlight the biocomplexity of the interactions between vibrios and their natural environment in a climate change scenario, posing the need for interdisciplinary studies to properly understand the connection between ocean warming and persistence and spread of vibrios in sea waters and the epidemiology of the diseases they cause.

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“…It is therefore crucial to eliminate any active cells from the dormant culture before carrying out the resuscitation procedure as the presence of only a few culturable cells in a VBNC cell suspension of interest could give false resuscitation results. 5 In the past, many techniques have been employed to prepare, isolate, and study bacterial cells that are viable but unable to multiply. [1][2][3]6,7 Unfortunately, many of these techniques are time consuming and labor intensive, and it is therefore necessary to look for rapid techniques for the separation of dormant from nondormant cells.…”

Section: Introductionmentioning

confidence: 99%

Separation by dielectrophoresis of dormant and nondormant bacterial cells of Mycobacterium smegmatis

Zhu¹,

Kaprelyants²,

Salina³

et al. 2010

Biomicrofluidics

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The dielectrophoretic behavior of active, dead, and dormant Mycobacterium smegmatis bacterial cells was studied. It was found that the 72-h-old dormant cells had a much higher effective particle conductivity ͑812Ϯ 10 S cm −1 ͒, almost double that of active cells ͑560Ϯ 20 S cm −1 ͒, while that of dead ͑autoclaved͒ M. smegmatis cells was the highest ͑950Ϯ 15 S cm −1 ͒ overall. It was also found that at 80 kHz, 900 S cm −1 dead cells were attracted at the edges of interdigitated castellated electrodes by positive dielectrophoresis, but dormant cells were not. Similarly, at 120 kHz, 2 S cm −1 active cells were attracted and dormant cells were not. Using these findings a dielectrophoresis-based microfluidic separation system was developed in which dead and active cells were collected from a given cell suspension, while dormant cells were eluted.

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Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation?

Cited by 57 publications

References 35 publications

Changes in the Vibrio harveyi Cell Envelope Subproteome During Permanence in Cold Seawater

Changes in the Vibrio harveyi Cell Envelope Subproteome During Permanence in Cold Seawater

Effects of Global Warming onVibrioEcology

Separation by dielectrophoresis of dormant and nondormant bacterial cells of Mycobacterium smegmatis

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