Recovery and separation of surfactin from pretreated fermentation broths by physical and chemical extraction

Chen, Huei-Li; Juang, Ruey‐Shin

doi:10.1016/j.bej.2007.06.003

Cited by 54 publications

(44 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of ethyl acetate resulted in a greater activity of crude extract when compared with systems based on mixtures of chloroform and methanol, cold acetone or dichloromethane (data not shown). It was also reported previously that the extraction of bioproducts with considerably high polarity by ethyl acetate solvent is rather efficient (Chen and Juang 2008). Because the recovery and concentration of biosurfactants from fermentation broth largely determines the production cost, ethyl acetate is a better choice than the highly toxic chloro-organic compounds.…”

Section: Recovery Of Biosurfactantmentioning

confidence: 94%

An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand

Saimmai

Onlamool

Sobhon

et al. 2012

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Biosurfactant-producing bacteria, isolate CT2, was isolated from mangrove sediment in the south of Thailand. The sequence of the 16S rRNA gene from isolate CT2 showed 100 % similarity with Selenomonas ruminantium. The highest biosurfactant production (5.02 g/l) was obtained when the cells were grown on minimal salt medium containing 15 g/l molasses and 1 g/l commercial monosodium glutamate supplemented with 1 g/l NaCl, 0.1 g/l leucine, 5 % (v/v) inoculum size at 30 °C and 150 rpm after 54 h of cultivation. The biosurfactant obtained by extraction with ethyl acetate showed high surface tension reduction (25.5 mN/m), a small CMC value (8 mg/l), thermal and pH stability with respect to surface tension reduction and emulsification activity and a high level of salt tolerance. The biosurfactant obtained was confirmed as a lipopeptide by using a biochemical test, FT-IR, MNR and mass spectrometry. The crude biosurfactant showed a broad spectrum of antimicrobial activity and also had the ability to emulsify oil and enhance PAHs solubility.

show abstract

Section: Recovery Of Biosurfactantmentioning

confidence: 94%

An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand

Saimmai

Onlamool

Sobhon

et al. 2012

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…They observed the adsorption of surfactin onto the surface of the fibers, rather than its extraction by nhexane followed by transportation through the pores of the fibers into the bulk n-hexane phase. Chen and Juang reported on the recovery of surfactin by physical and chemical extraction [108]. They performed physical solid-liquid and liquid-liquid extractions with different organic solvents (ethyl acetate, n-hexane) for different durations.…”

Section: Extractionmentioning

confidence: 99%

“…The function of surfactin function is attributable to its ability to form of ion-conducting channels in bacterial lipid bilayer membranes by detergent-like action thereon [114][115][116][117]. This detergent-like action makes surfactin a potent inhibitor of the cyclic adenosine 3, 5,-monophosphate phosphodiesterase enzyme, facilitating the chelating action of the free carboxyl groups of glutamic acid and aspartic acid residues of surfactin [108].…”

Section: Antimycoplasmal Antibacterial and Antiviral Activitymentioning

confidence: 99%

Applications of a lipopeptide biosurfactant, surfactin, produced by microorganisms

Chen

Juang

Wei

2015

Biochemical Engineering Journal

Self Cite

218

113

View full text Add to dashboard Cite

“…However, the recovery of carboxylic acids from dilute aqueous solutions with high efficiency is still a challenging separation problem (Wasewar et al, 2004;Lopez-Garzon and Straathof, 2014;Uslu et al, 2015;Datta et al, 2016). Moreover, most of the industrial separation operations are carried out using toxic chemicals (Harington and Hossain, 2008;Keshav et al, 2009) and the cost of these processes are generally ≥40% of the total cost (Chen and Juang, 2008;Straathof, 2014). Therefore, several researchers from all over the world have been studying the recovery of carboxylic acids from production media and wastewaters, or aqueous solutions having similar characteristics (concentration, pH and etc.…”

Section: Introductionmentioning

confidence: 99%

Recovery of Formic Acid by Reactive Extraction Using an Environmentally-Friendly Solvent

Martı¹

2017

Scitech

View full text Add to dashboard Cite

Today many carboxylic acids are recovered from biological production media and industrial wastewaters. Reactive extraction is favored over other separation methods due to its high recovery efficiency, ease of operation, low energy demand and reduced cost. However, use of toxic organic diluents in the organic phases is the main disadvantage of the method. In this study, the appropriateness of an environmentally-friendly solvent, sunflower oil (SFO) to be used as organic phase diluent during the recovery of formic acid (FA) from aqueous solutions by reactive extraction was evaluated. Alamine-336 was used as the extractant and the results obtained with SFO were compared with those obtained using octanol. The separation process reached an equilibrium in 3 hours and the recovery efficiency increased with the increase in extractant percentages. The initial FA concentration positively influenced the recovery when SFO was used as the diluent. In the ranges of the parameters studied, the highest recovery values were 98.6% and 82.6% with octanol and SFO, respectively. This study shows that low molecular weight carboxylic acids, e.g. FA can be recovered from aqueous solutions by reactive extraction using environmentally-friendly solvents such as SFO. Key Words: Formic acid, Reactive extraction, Sunflower oil, Alamine-336, EquilibriumFormik Asidin Çevre Dostu Bir Çözücü Kullanılarak Tepkimeli Özütleme Yöntemi ile Geri Kazanımı ÖZ: Günümüzde pek çok karboksilik asit biyolojik üretim ortamlarından ve endüstriyel atık sulardan geri kazanılmaktadır. Tepkimeli özütleme, yüksek geri kazanım verimi, işlem basitliği, düşük enerji gereksinimi ve maliyeti nedeniyle diğer ayırma yöntemlerine tercih edilmiştir. Fakat organik fazlarda zehirli organik seyrelticilerin kullanımı yöntemin en önemli dezavantajıdır. Bu çalışmada çevre dostu bir çözücünün, ayçiçek yağının formik asidin sulu çözeltilerinden tepkimeli özütleme yöntemi ile geri kazanımı sırasında organik faz seyrelticisi olarak zehirli organik kimyasallar yerine kullanımı değerlendirilmiştir. Özütleyici olarak Alamine-336 kullanılmış ve ayçiçek yağı ile elde edilen sonuçlar oktanol ile elde edilen sonuçlarla karşılaştırılmıştır. Ayırma süreci 3 saatte dengeye ulaşmış ve geri kazanım verimi özütleyici yüzdesi ile birlikte artmıştır. Ayçiçek yağı seyreltici olarak kullanıldığında başlangıç asit derişimi geri kazanımı pozitif yönde etkilemiştir. Çalışılan değişken aralıklarında oktanol ve ayçiçek yağı ile en yüksek geri kazanım verimleri sırasıyla %98,6 ve %82,6'dır. Bu çalışma formik asit gibi düşük molekül ağırlıklı karboksilik asitlerin tepkimeli özütleme ile ayçiçek yağı gibi çevre dostu çözücüler kullanılarak sulu çözeltilerden geri kazanılabileceğini göstermiştir.

show abstract

Recovery and separation of surfactin from pretreated fermentation broths by physical and chemical extraction

Cited by 54 publications

References 28 publications

An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand

An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand

Applications of a lipopeptide biosurfactant, surfactin, produced by microorganisms

Recovery of Formic Acid by Reactive Extraction Using an Environmentally-Friendly Solvent

Contact Info

Product

Resources

About