Applications of a lipopeptide biosurfactant, surfactin, produced by microorganisms

Chen, Wei‐Chuan; Juang, Ruey‐Shin; Wei, Yuhong

doi:10.1016/j.bej.2015.07.009

Cited by 219 publications

(150 citation statements)

References 128 publications

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“…Surfactin is a representative lipopeptide; it has a seven amino acid circular hydrophilic head, and the greatest surface activity (Jacques, ; Peypoux et al, ) and stability of all known kipopeptides (Bonmatin et al, ). Recent reports have further highlighted its huge potential in enhancing oil recovery (Cheng et al, ; Li et al, ; Pereira et al, ) and in pharmaceuticals, for example, in oral insulin delivery (Chen et al, ; Gao et al, ).…”

Section: Discussionmentioning

confidence: 99%

In situ enhancement of surfactin biosynthesis in Bacillus subtilis using novel artificial inducible promoters

Song

et al. 2016

Biotech & Bioengineering

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Surfactin-family lipopeptides are green biosurfactants with substantial industrial potential. The major problem prohibiting surfactin use is the low titer of the wild producer, Bacillus subtilis. Using transcriptomic analysis, four strong promoters, PgroE, Pcdd, PrplK, and PsspE, were identified and cloned from the genome of B. subtilis THY-7, a novel surfactin producer that has been identified from soil with a 0.55 g/L surfactin titer. An optimal promoter, PgroE, was selected to replace the native THY-7 surfactin synthase (SrfA) promoter through single-cross homologous recombination; however, the resulting engineered strain containing the PgroE substitution did not synthesize surfactin. The sucrose-inducible promoters PsacB and PsacP were then substituted in place of PsrfA, and the resulting engineered strains produced 1.09 and 0.22 g/L surfactin, respectively. An artificial, sucrose-inducible Pg1 promoter was produced through fusion of the PgroE and PsacB ribonucleic antiterminator (RAT), and the engineered strain containing the Pg1-substitution produced a surfactin titer of 1.44 g/L. An artificial IPTG-inducible promoter, Pg2, was constructed from a PgroE-lacO fusion and then substituted for the chromosomal PsrfA locus, and the surfactin titer of the resulting THY-7/Pg2-srfA increased to 5.98 g/L. The driving capacity of Pg2 was further improved by the inclusion of two point mutations in the -35 and -10 regions to produce the novel promoter Pg3. Pg3 exhibited super-strong activity as measured by lacZ reporter gene overexpression (approximately 3000 U). The Pg3-substitution strain THY-7/Pg3-srfA produced up to 9.74 g/L surfactin in a 5 L fermentor. The maximum productivity was 0.30 g/L/h, and the greatest yield reached 0.14 g surfactin/g sucrose. Biotechnol. Bioeng. 2017;114: 832-842. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

In situ enhancement of surfactin biosynthesis in Bacillus subtilis using novel artificial inducible promoters

Song

et al. 2016

Biotech & Bioengineering

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“…These results were similar to the reports of previous studies (Sahnoun et al 2014;Shi et al 2014a;Li 2015;Zhai et al 2015). They contributed this behaviour of surfactin to its surface activity, because all the biological activity of surfactin is based on the surface activity, and surfactin interaction with the cell membrane is highly dependent on the surfactin concentration, a threshold concentration of surfactin in the bilayer is needed for its solubilization (Seydlova & Svobodova 2008;Chen et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, surfactin has been characterized as anti-bacterial, anti-viral, anti-fungal, biosurfactant, immunoadjuvant, anti-tumor, antimycoplasma and hemolytic activities. The potential applications of surfactin are therapeutic applications and environmental applications (Seydlova & Svobodova 2008;Gao et al 2013;Chen et al 2015). Little information is available about surfactin application in feed of aquatic animals.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary surfactin supplementation on growth performance, intestinal digestive enzymes activities and some serum biochemical parameters of tilapia (Oreochromis niloticus) fingerlings

Zhai

Shi

Chen

2016

Italian Journal of Animal Science

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This trial was conducted to investigate the effects of supplementary surfactin on growth performance, intestinal digestive enzymes activities and some serum biochemical parameters of tilapia (Oreochromis niloticus) fingerlings. Three hundred and twenty fish were randomly divided into four treatment groups with four replicates in each group and 20 fish in each replicate. The dietary surfactin levels of four treatment groups were 0 (control group), 50, 100 and 200 mg/kg. The trial period was 7 weeks. Compared with the control group, final body weight, daily feed intake, weight gain rate, feed conversion ratio and protein efficiency ratio were improved significantly by surfactin supplementation (p < 0.05). No significant differences of survival rate were found between the control group and all surfactin supplementation groups (p > 0.05). The activities of protease and lipase in intestine of surfactin supplementation groups were significantly higher than those of the control group (p < 0.05), the amylase activities were similar among all treatment groups (p > 0.05). The activities of aspartate aminotransferase and alanine aminotransferase were decreased significantly by dietary surfactin supplementation (p < 0.05), the levels of blood urea nitrogen, triglyceride, total cholesterol and low-density lipoprotein cholesterol were also decreased significantly (p < 0.05), while lysozyme activity and the high-density lipoprotein cholesterol level were increased significantly by the surfactin supplementation (p < 0.05). In conclusion, we demonstrated a promotion of growth performance and improvement of intestinal digestive enzymes activities and some serum biochemical parameters of tilapia fingerlings by dietary surfactin supplementation.ARTICLE HISTORY

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“…Todavia, a utilização de biossurfactantes é promissora em produtos de elevado valor agregado, como cosméticos e medicamentos. 3 A surfactina foi relatada pela primeira vez em estudo em que se pesquisou um inibidor da formação do coágulo de fibrina e se isolou um lipopeptídeo cristalino de um cultivo de B. subtilis com potente ação inibidora da formação de coágulo e como agente tensoativo de superfície. 4 A surfactina diluída em água destilada (0,005%) reduziu a tensão superficial da água de 72 mN m -1 para 27 mN m -1 , e esse resultado foi superior ao observado para o lauril sulfato de sódio, um surfactante sintético.…”

Section: Introductionunclassified

Surfactina: estrutura, aplicações e fatores envolvidos em sua produção

Larini

Rezende

Ribeiro

et al. 2017

evid.

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ResumoA surfactina é um lipopeptídeo da classe dos biossurfactantes, mais comumente produzida por Bacillus subtilis, uma bactéria Gram-positiva. A estrutura química da surfactina é composta por peptídeo ligado aos grupos carboxil e hidrocarboxil de ácidos graxos de cadeia longa. A produção de surfactina pode ser influenciada pela linhagem, inóculo e idade do microrganismo, além de fatores físicos como temperatura, pH, velocidade de agitação, grau de aeração, formação de espuma e concentração de oxigênio dissolvido e fatores nutricionais, como fontes de carbono, nitrogênio e elementos-traço nos meios de cultivo. As potenciais áreas de aplicação desse biossurfactante são terapêuticas e ambientais. As surfactinas possuem análogos estruturais ou isoformas A, B e C, conforme a sequência de aminoácidos e o número de átomos de carbono do ácido graxo. 9 A surfactina A é a principal isoforma e apresenta a sequência de resíduos de aminoácidos-Leu-C em sua porção peptídica e difere no sétimo aminoácido, sendo Val e Ile para as isoformas B e C. 10 A composição da porção peptídica e de ácidos graxos β-hidróxi da molécula dependem das linhagens bacterianas produtoras e das condições de cultivo. 11Pesquisadores cultivaram B. subtilis HSO121 e identificaram três surfactinas com diferentes porções peptídicas, sendo surfactina A (N-Glu-Leu-Leu-Val-Asp-Leu-Leu-C), uma surfactina com a sequência N-Asp-Leu-Leu-Val-Glu-Leu-Leu-C e outra com uma cadeia peptídica metil esterificada. 12Os ácidos graxos identificados foram β-hidróxi iso C 12 , iso C 13 , anteiso C 13 , iso C 14 , iso C 15 , anteiso C 15 , n C 15 , anteiso C 16 e anteiso C 17 . Para compreender o processo pelo qual os microrganismos produzem surfactina, nesta seção apresentam-se vários aspectos de sua produção, incluindo as condições de cultivo e composição dos meios utilizados. Entre os fatores que afetam a produção de surfactina, tem-se: tipo de microrganismo, condições físicas e nutricionais e substratos utilizados na produção. MICRORGANISMOS PRODUTORESO Bacillus subtilis tem sido amplamente estudado e é o microrganismo mais importante para a produção de surfactina.14,5 Os B. subtilis são bastonetes, Gram-positivos, encontradas em solo e vegetação, crescem na temperatura entre 25 e 37 ºC e são capazes de desenvolver estratégias que permitem sua sobrevivência em condições extremas, como estresse ambiental e falta de nutrientes. 3 Na Tabela 1 observam-se as linhagens de B. subtilis empregadas para a produção de surfactina, os substratos utilizados e as concentrações obtidas.

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Applications of a lipopeptide biosurfactant, surfactin, produced by microorganisms

Cited by 219 publications

References 128 publications

In situ enhancement of surfactin biosynthesis in Bacillus subtilis using novel artificial inducible promoters

In situ enhancement of surfactin biosynthesis in Bacillus subtilis using novel artificial inducible promoters

Effects of dietary surfactin supplementation on growth performance, intestinal digestive enzymes activities and some serum biochemical parameters of tilapia (Oreochromis niloticus) fingerlings

Surfactina: estrutura, aplicações e fatores envolvidos em sua produção

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