Recovery and purification of chitosanase produced by <i>Bacillus cereus</i> using expanded bed adsorption and central composite design

Araújo, Nathália Kelly de; Pimentel, Vanessa Carvalho; Silva, Nayane Macedo Portela da; Padilha, Carlos Eduardo de Araújo; Macêdo, Gorete Ribeiro de; Santos, Everaldo Silvino dos

doi:10.1002/jssc.201500900

Cited by 12 publications

(6 citation statements)

References 35 publications

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“…The different effect of cation upon catalytic action of the enzyme may be due to their ability to adopt different geometrics in the same site in the absence of substrate [42]. The stimulatory effect of Mn 2+ on chitosanases was also reported by other authors [43][44][45]. A B Fig.…”

Section: Effect Of Metal Ions On Free and Immobilized Enzymesupporting

confidence: 53%

Covalent immobilization of Dothideomycetes sp. NRC-SSW chitosanase and its application in chitosan hydrolysis

2021

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Chitosanases are chitosan specific glycosyl hydrolases that can exploit in several biotechnological applications. In the current study, a modified bead of carrageenan and carboxymethyl cellulose was used for the covalent immobilization of Dothideomycetes sp. NRC-SSW chitosanase. The immobilization process was optimized and the immobilization recovery was increased up to 69%. The stepwise different bead formulations were examined under Scanning Electron Microscope, Fourier Transform Infrared Spectroscopy and Thermal Gravimetric analysis, confirming the success of the immobilization process. Additionally different properties including enzyme storage, thermal and pH stabilities were examined and an improvement in all of these properties was estimated. Although the same Vmax value (23.8U/min/mg protein) was recorded for the free and the immobilized enzymes, the Km was 10mg chitosan/ml and 16.67mg chitosan/ml for the immobilized and free form respectively. The activation energy for chitosan hydrolysis using the immobilized enzyme (12.497kJmol −1 ) was less than the third of that recorded for the free one (42.196kJmol −1 ). Furthermore, the immobilized enzyme was stable until the 5 th cycle at which it retained more than 50% of its original activity. All the tested parameters confirmed that the immobilized Dothideomycetes sp. NRC-SSW chitosanase was more applicable for chitosan hydrolysis than the native enzyme.

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Section: Effect Of Metal Ions On Free and Immobilized Enzymesupporting

confidence: 53%

Covalent immobilization of Dothideomycetes sp. NRC-SSW chitosanase and its application in chitosan hydrolysis

2021

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“…suspensions containing different particulates, flow rates, viscosities, etc. [41]. Accordingly, the results obtained in this work are compared with past data reported for CPC purification using Q and DEAE Streamline media in EBA mode ( Table 2).…”

Section: Initial Purity (A 620 /A 280 ) [-]mentioning

confidence: 81%

Demonstration of protein capture and separation using three‐dimensional printed anion exchange monoliths fabricated in one‐step

Simon

Scorza

Teworte

et al. 2020

J of Separation Science

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Three-dimensional printing applications in separation science are currently limited by the lack of materials compatible with chromatographic operations and three-dimensional printing technologies. In this work, we propose a new material for Digital Light Processing printing to fabricate functional ion exchange monoliths in a single step. Through copolymerization of the bifunctional monomer [2-(acryloyloxy)ethyl] trimethylammonium chloride, monolithic structures with quaternary amine ligands were fabricated. The novel formulation was optimized in terms of protein binding and recovery, microporous structure, and its swelling susceptibility by increasing its cross-link density and employing cyclohexanol and dodecanol as pore forming agents. In static conditions, the material demonstrated a maximum binding capacity of 104.2 ± 10.6 mg/mL for bovine serum albumin, in line with commercially available materials. Its anion exchange behavior was validated by separating bovine serum albumin and myoglobin on a monolithic bed with Schoen gyroid morphology. The same column geometry was tested for the purification of C-phycocyanin from clarified as well as cell-laden Arthrospira platensis feedstocks. This represents the first demonstration of one-step printed stationary phases to capture proteins directly from solid-laden feedstocks. We believe that the material presented here represents a significant improvement towards implementation of three-dimensional printed chromatography media in the field of separation science.

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“…Chitosanases also have a function in plant pathogen suppression ( Zhao et al., 2011 ). In prokaryotes, chitosanase, whose target chitosan is not a constituent of the cells, is alleged to work as extracellular enzymes ( de Araújo et al., 2016 ; Park et al., 1999 ; Liang et al., 2014 ). A gene cluster encoding chitosanase, which was able to prevent plant from the infection by Plasmodiophora brassicae , a common pathogen that causes clubroot disease, has been identified ( Guo et al., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a novel strain of Bacillus halotolerans protecting wheat from sheath blight disease caused by Rhizoctonia solani Kühn

Feng¹,

Xu²,

Yang³

et al. 2022

Front. Plant Sci.

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Rhizoctonia solani Kühn naturally infects and causes Sheath blight disease in cereal crops such as wheat, rice and maize, leading to severe reduction in grain yield and quality. In this work, a new bacterial strain Bacillus halotolerans LDFZ001 showing efficient antagonistic activity against the pathogenic strain Rhizoctonia solani Kühn sh-1 was isolated. Antagonistic, phylogenetic and whole genome sequencing analyses demonstrate that Bacillus halotolerans LDFZ001 strongly suppressed the growth of Rhizoctonia solani Kühn sh-1, showed a close evolutionary relationship with B. halotolerans F41-3, and possessed a 3,965,118 bp circular chromosome. Bioinformatic analysis demonstrated that the genome of Bacillus halotolerans LDFZ001 contained ten secondary metabolite biosynthetic gene clusters (BGCs) encoding five non-ribosomal peptide synthases, two polyketide synthase, two terpene synthases and one bacteriocin synthase, and a new kijanimicin biosynthetic gene cluster which might be responsible for the biosynthesis of novel compounds. Gene-editing experiments revealed that functional expression of phosphopantetheinyl transferase (SFP) and major facilitator superfamily (MFS) transporter genes in Bacillus halotolerans LDFZ001 was essential for its antifungal activity against R. solani Kühn sh-1. Moreover, the existence of two identical chitosanases may also make contribution to the antipathogen activity of Bacillus halotolerans LDFZ001. Our findings will provide fundamental information for the identification and isolation of new sheath blight resistant genes and bacterial strains which have a great potential to be used for the production of bacterial control agents.ImportanceA new Bacillus halotolerans strain Bacillus halotolerans LDFZ001 resistant to sheath blight in wheat is isolated. Bacillus halotolerans LDFZ001 harbors a new kijanimicin biosynthetic gene cluster, and the functional expression of SFP and MFS contribute to its antipathogen ability.

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Recovery and purification of chitosanase produced by Bacillus cereus using expanded bed adsorption and central composite design

Cited by 12 publications

References 35 publications

Covalent immobilization of Dothideomycetes sp. NRC-SSW chitosanase and its application in chitosan hydrolysis

Covalent immobilization of Dothideomycetes sp. NRC-SSW chitosanase and its application in chitosan hydrolysis

Demonstration of protein capture and separation using three‐dimensional printed anion exchange monoliths fabricated in one‐step

Molecular characterization of a novel strain of Bacillus halotolerans protecting wheat from sheath blight disease caused by Rhizoctonia solani Kühn

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