Recovery and cryopreservation of epididymal sperm from domestic cat using powdered coconut water (ACP-117c) and TRIS extenders

Barbosa, Brenna de Sousa; Izzo, Roberta; Silva, Herlon Victor Rodrigues; Nunes, Thalles Gothardo Pereira; Brito, Bruna Farias; Silva, Ticiana Franco Pereira da; Silva, Lúcia Daniel Machado da

doi:10.1016/j.cryobiol.2019.11.042

Cited by 11 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The integrity of the chromatin was maintained after thawing in all treatments. Previous studies report the excellent stability and cryoresistance of feline sperm chromatin, not distancing from the values observed in this study (Manee-In et al, 2014;Kunkitti et al, 2016;Barbosa et al, 2020). Sperm chromatin is a cluster of DNA, RNA and proteins that are located inside the nucleus of the sperm.…”

Section: Discussionsupporting

confidence: 69%

“…In addition, the composition of epididymal fluids may have inferred the response/interaction of the sperm cell with propolis, and consequent sperm morphology. Even with the reduction in the percentage of normal sperm, P1 and P2 groups remained within the limits reported for the cryopreserved sample (40% -54.5%) (Cocchia et al, 2009;Jiménez et al, 2013; Research, Society and Development, v. 10, n. 2, e48710212842, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i2.12842 Barbosa et al, 2020).…”

Section: Discussionsupporting

confidence: 61%

“…The spermatozoa were recovered from cauda epididymis by slicing technique, in a Petri dish containing 1 mL of Tris buffer solution (Tris-hydroxymethyl-aminomethane plus fructose and citric acid monohydrate) at 37ºC (Barbosa et al, 2020).…”

Section: Epididymal Sperm Collection Cryopreservation and Thawingmentioning

confidence: 99%

See 2 more Smart Citations

Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa

Gomes

Fernandes

Izzo

et al. 2021

RSD

Self Cite

View full text Add to dashboard Cite

This study evaluated the effect of different concentrations of Brazilian green propolis on the cryopreservation of epididymal sperm from domestic cats. Spermatozoa were collected from cauda epididymis by slicing technique in a TRIS extender, preheated to 37ºC. Then, the sperm were evaluated and divided randomly between the control group (without adding propolis in the extender) and the treatment groups (adding different concentrations of propolis extract in the extender): P1 (0.1 mg/mL), P2 (0.3 mg/mL) e P3 (0.6 mg/mL). Subsequently, sperm were cryopreserved. The evaluated parameters were sperm kinetics by the Computer Assisted Semen Analysis, vigor, viability, membrane functionality, chromatin condensation and morphology. The parameters were measured before and after cryopreservation. Propolis didn’t show toxicity to the sperm in any concentrations, with no changes observed in the motility pattern. There was no significant influence of propolis on motility, vigor, percentage of viable spermatozoa, chromatin quality and other kinetic parameters in the cryopreserved samples. In sperm morphology, it was demonstrated a reduction in the percentage of normal cells in P1 and P2 groups in relation to the fresh sample, control and P3 group. In addition, an interesting effect from the propolis groups was observed on possible growth inhibition of microorganisms in the contaminated samples. Propolis didn’t provide superiority to the sperm parameters evaluated after thawing, except for the values of plasma membrane functionality, which were better. The propolis is an interesting component to be incorporated in the cryoprotectant medium due to its non-toxicity and the potential inhibition of microbial growth.

show abstract

Section: Discussionsupporting

confidence: 69%

Section: Discussionsupporting

confidence: 61%

See 1 more Smart Citation

Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa

Gomes

Fernandes

Izzo

et al. 2021

RSD

Self Cite

View full text Add to dashboard Cite

show abstract

“…Finally, regarding CASA results, the values recorded for motility and kinetic velocity parameters are equivalent to those obtained in previous studies, both for TRIS and ACP-106c extenders (Uchoa et al, 2012;Costa et al, 2013). The ACP-106c group showed lower ALH than TRIS, which is not good regarding the functionality of the gamete, since this parameter is closely related to the ability of the sperm to penetrate in vivo and in vitro and to the gamete hyperactivation process (Cardoso et al, 2006;Barbosa et al, 2020). Another divergent kinetic variable among the evaluated extenders was linearity, which was higher in the ACP-106c group.…”

Section: Discussionmentioning

confidence: 98%

Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS

et al. 2022

Self Cite

View full text Add to dashboard Cite

Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assisted semen analysis (CASA), sperm viability, membrane functionality and sperm morphometry parameters of cryopreserved canine semen. Hence, we aimed to evaluate the effects of ACP-106c and TRIS on post-freezing canine sperm quality. Five dogs were submitted to semen collection twice with one-week interval. The semen was evaluated within the parameters: total motility, vigor, concentration, viability, plasma membrane functionality, morphology and morphometry. In the morphometric evaluation, the morphologically normal sperm was measured as: length, width, area and perimeter of the head and the midpiece, tail length and total length. The parameters of ellipticity, elongation, regularity and roughness were determined. Then, the semen was divided into two aliquots that were diluted in TRIS or ACP-106c, with the addition of egg yolk and glycerol. The diluted semen was refrigerated and frozen. The thawed samples were evaluated. Total motility, viability, sperm membrane functionality and normal morphology reduced after thawing in both extenders (morphology reduced from 89.60 ± 1.3% to 84.40 ± 1.8 and 84.60 ± 1.1% in TRIS and ACP-106c, respectively). However, it did not differ between TRIS and ACP-106c. In the ACP-106c the sperm head defects in cryopreserved semen were higher compared to fresh semen (P < 0.05). For all the morphometric parameters evaluated, there were no differences between fresh and cryopreserved samples (3.70 ± 0.4% vs. 2.30 ± 0.5%). In kinetics, with an interval of one week statistical differences between the extenders were found only in the parameters ALH and LIN (P < 0.05). Regardless of the extender, there were no changes in the morphometric parameters of sperm after thawing.

show abstract

“…These authors observed better motility (46.0% vs. 20.9%), membrane functionality (60.5% vs. 47.1%), and mitochondrial activity (21.5% vs. 11.8%) in samples cryopreserved in Tris rather than those cryopreserved in ACP-117c. Similarly, Tris promoted better preservation of the quality of frozen-thawed sperm from domestic cats compared to that by ACP-117c (Barbosa et al, 2020).…”

Section: Discussionmentioning

confidence: 98%

Heterologous in vitro fertilization and embryo production for assessment of jaguar (Panthera onca Linnaeus, 1758) frozen-thawed semen in different extenders

et al. 2022

Self Cite

View full text Add to dashboard Cite

Heterologous in vitro fertilization (IVF) is an important tool for assessing fertility of endangered mammals such as the jaguar, considering difficult access to females for artificial insemination and to obtain homologous oocytes. We aimed to evaluate the fertility of jaguar sperm cryopreserved with different extenders, using domestic cat oocytes to assess the development of hybrid embryos. Semen from four captive jaguars was obtained by electroejaculation. Samples were cryopreserved in powdered coconut water (ACP-117c) or Tris extender containing 20% egg yolk and 6% glycerol. Thawed spermatozoa were resuspended (2.0 × 10 6 spermatozoa/mL) in IVF medium and co-incubated with cat oocytes matured in vitro for 18 h. Presumptive zygotes were cultured for 7 days. After 48 h, cleavage rate was evaluated, and non-cleaved structures were stained for IVF evaluation. On days 5 and 7, the rate of morula and blastocyst formation was assessed. Data were analyzed using the Fisher exact test (p < 0.05). No difference was observed between ACP-117c and Tris extenders, respectively, for oocytes with 2nd polar body (2/51, 3.9 ± 2.9% vs. 2/56, 3.6 ± 3.1%), pronuclear structures (5/51, 9.8 ± 4.7% vs. 8/56, 14.3 ± 8.0%), and total IVF rates (7/36, 19.4 ± 5.0% vs. 10/37, 27.0 ± 13.8%). All the samples fertilized the oocytes, with 22.9 ± 3.2% (16/70) and 16.7 ± 3.6% (12/72) cleavage of mature oocytes for ACP-117c and Tris extenders, respectively. Morula rates of 4.3 ± 2.3% (3/70) and 5.6 ± 2.2% (4/72) were observed for ACP-117c and Tris, respectively. Only the Tris extender demonstrated blastocyst production (2/12, 16.7 ± 1.5% blastocyst/cleavage). We demonstrated that jaguar ejaculates cryopreserved using ACP-117c and Tris were suitable for IVF techniques, with blastocyst production by ejaculates cryopreserved in Tris. This is a first report of embryos produced in vitro using jaguar sperm and domestic cat oocytes through IVF.

show abstract

Recovery and cryopreservation of epididymal sperm from domestic cat using powdered coconut water (ACP-117c) and TRIS extenders

Cited by 11 publications

References 28 publications

Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa

Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa

Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS

Heterologous in vitro fertilization and embryo production for assessment of jaguar (Panthera onca Linnaeus, 1758) frozen-thawed semen in different extenders

Contact Info

Product

Resources

About