Recording of lymph flow dynamics in microvessels using correlation properties of scattered coherent radiation

Fedosov, Ivan V.; Тучин, В. В.; Galanzha, Ekaterina I.; Solov'eva, Anastasiya V.; Stepanova, Tatyana

doi:10.1070/qe2002v032n11abeh002330

Cited by 14 publications

(15 citation statements)

References 9 publications

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“…In particular, the values of average diastolic lymph fl ow velocity of 0.23 mm/s and maximal systolic lymph fl ow velocity of 1.81 mm/s correlated to those observed in young Sprague-Dawley rats reported in several studies (Dixon et al 2006 ;Brill et al 2001 ;Galanzha et al 2002Galanzha et al , 2005Fedosov et al 2002 ) and in young F-344 rats (Galanzha et al 2005 ). The average values of diastolic and maximal systolic wall shear stress, 0.61 dyn/cm 2 and 5.15 dyn/cm 2 , respectively, were also found to be similar to the ranges determined in young Sprague-Dawley rats (Dixon et al 2006 ), which strengthen our general conclusions on the similar lymph contractility and fl ow patterns within young and adult rats of different strains.…”

Section: Discussionsupporting

confidence: 76%

Immunology of the Lymphatic System

Santambrogio¹

2013

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Section: Discussionsupporting

confidence: 76%

Immunology of the Lymphatic System

Santambrogio¹

2013

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“…To increase its ability to measure the absolute value and direction of flow, we also used the specklecorrelation mode, which uses two photodetectors to record the intensity in fluctuations of the speckle field at two points [123,124] . The described algorithm was verified in vivo by measuring lymph flow in a lymphatic vessel with LSM ( Figure 7F curve 1) and TDM ( Figure 7F curve 2).…”

Section: Laser Speckle Microscopy (Lsm) Modulementioning

confidence: 99%

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

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Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo . Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell's functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo-and radio-sensitivity tests, and drug screening, are also discussed. TOPIC HIGHLIGHT

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“…6) 20 The experimental setup was based on a microscope used for studying the lymph flow dynamics in the vessels of rat mesentery with transmission functional microscopy technique. The setup allows us to perform experiments with blood and lymph vessel models and also to study real microvessels in vivo.…”

Section: Methodsmentioning

confidence: 99%

“…'3 Detection oflaser light scattered by blood or lymph vessel in forward direction could be realized in very specific conditions e. g. on the animal models such as rat mesentery ( fig.1 . (b))'°' 20 And the most widespread, but less informative geometry for blood flow investigation is the light scattering by several tissue volume containing a number of blood vessels ( fig. 1(c)).…”

Section: Introductionmentioning

confidence: 99%

<title>Laser measurements for biomedical applications</title>

Fedosov

Tuchin

2006

SPIE Proceedings

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Laser Doppler and laser speckle techniques for the measurements of blood and lymph flow velocity in different geometry has been overviewed and compared. The application of laser Doppler techniques for the diagnostics of blood microcirculation disorders in ophthalmology, otorhinolaryngology and dentistry are described. Also the application of laser speckle-correlation technique for the biomedical studies of lymphatic microcirculation is presented. Blood flow measurements of both single vessel and tissue volume is discussed.

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Recording of lymph flow dynamics in microvessels using correlation properties of scattered coherent radiation

Cited by 14 publications

References 9 publications

Immunology of the Lymphatic System

Immunology of the Lymphatic System

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

<title>Laser measurements for biomedical applications</title>

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