Reconstruction of mandibular defects with autologous tissue-engineered bone

Abukawa, Harutsugi; Shin, Michael; Williams, W. Bradford; Vacanti, Joseph P.; Kaban, Leonard B.; Troulis, Maria J.

doi:10.1016/j.joms.2003.11.010

Cited by 129 publications

(67 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because of these characteristics MSC are regarded as possible effectors for tissue repair in vivo, tissue engineering and as target cells for gene therapy. [38][39][40] In recent preclinical and clinical studies cultureexpanded MSC were systemically administered to animals or patients. The establishment of a biological/clinical effect following this route of administration necessitates that MSC are capable of crossing the endothelial barrier lining blood vessels in order to reach a specific tissue.…”

Section: Discussionmentioning

confidence: 99%

Migration of culture-expanded human mesenchymal stem cells through bone marrow endothelium is regulated by matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-3

Becker¹,

Hummelen²,

Bâkkus³

et al. 2007

Haematologica

209

149

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Migration of culture-expanded human mesenchymal stem cells through bone marrow endothelium is regulated by matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-3

Becker¹,

Hummelen²,

Bâkkus³

et al. 2007

Haematologica

209

149

View full text Add to dashboard Cite

“…Nos últimos anos, mundialmente são descritos experimentos que visam a reprodução total ou parcial de estruturas anatômica humana, como por exemplo: tecido hepático [63] , dente [64] , mandíbula [65] , nervos periféricos [66] , válvulas e ductos urológicos [67] , menisco [68] , vasos [69] e regeneração de tecido ósseo [70] .…”

Section: Aplicações E Produtos Da Engenharia De Tecidosunclassified

Polímeros bioreabsorvíveis na engenharia de tecidos

Barbanti

Zavaglia

Duek³

2005

Polímeros

View full text Add to dashboard Cite

[3] , sistemas para liberação controlada de drogas [4] , stents [5] e dispositivos ortopédicos [6] . Atualmente fazem parte do cotidiano dos centros cirúrgicos no mundo inteiro.Embora muitos dispositivos protéticos artificiais estejam disponíveis, poucos podem substituir completamente todas as complexas funções biológicas. Em situações clíni-cas mais severas somente o transplante do órgão retoma as atividades orgânicas. Assim, de uma forma idealizada, a melhor alternativa seria obter um novo órgão ou tecido, substituindo aquele que não desempenha normalmente suas funções. Nos dias de hoje, a idéia da reconstrução de órgãos e tecidos criados em laboratório é amplamente difundida e investigada no mundo todo [7,8] . Resumo: A Engenharia de Tecidos consiste em um conjunto de conhecimentos e técnicas para a reconstrução de novos órgãos e tecidos. Baseada em conhecimentos das áreas de ciência e engenharia de materiais, biológica e médica, a técnica envolve a expansão in vitro de células viáveis do paciente doador sobre suportes de polímeros bioreabsorvíveis. O suporte degrada enquanto um novo órgão ou tecido é formado. Os poli(α-hidróxi ácidos) representam a principal classe de polímeros sintéticos bioreabsorvíveis e biodegradáveis utilizados na engenharia de tecidos. No desenvolvimento e na seleção desses materiais, o tempo de degradação é fundamental para o sucesso do implante. Os estudos e os desafios atuais são normalmente direcionados ao entendimento das relações entre composição química, cristalinidade, morfologia do suporte, e o processamento desses materiais. Este artigo faz uma revisão dos trabalhos recentes sobre a utilização dos polímeros sintéticos bioreabsorvíveis como suportes na engenharia de tecidos. Engenharia de tecidos Palavras-chave: Engenharia de tecidos, polímeros bioreabsorvíveis, poli(α-hidróxi ácidos). Bioresorbable Polymers in Tissue EngineeringAbstract: Tissue Engineering is based on a group of techniques for the reconstruction of new organs and tissues. Based on knowledge of materials science and engineering, biology and medicine, the technique involves the in vitro expansion of viable cells obtained from the patient on the polymeric scaffolds. The scaffold degrades while a new organ or tissue is formed. The poly(α-hydroxy acids) are the principal biodegradable and bioresorbable polymers used in tissue engineering. In developing and selecting bioresorbable scaffolds, the degradation time is fundamental for successful biocompatibility and biofuncionality. Hence, degradation studies often address variables such as the chemical composition, crystallinity, morphology of the scaffold and the processing of these materials. This paper reviews recent work in bioresorbable polymers used as scaffolds in the tissue engineering. Keywords: Tissue engineering, bioresorbable polymers, poly(α-hydroxy acids). IntroduçãoQuando a estrutura biológica de um órgão ou tecido não pode ser reparada, a alternativa viável para o restabelecimento das funções normais do paciente é repô-la com um implante feito de um b...

show abstract

“…Craniofacial bone grafts are unique in that their functionality is also linked to their overall geometry. Many studies have thus focused on the ability to recapitulate the anatomical shape of cell-based constructs in vitro (3)(4)(5)(6)(7). Bone grafts of the highest utility for reconstructive surgery would be based on "designer scaffolds" shaped into gross geometries specific to the patient and defect being treated (1,(8)(9)(10)(11).…”

mentioning

confidence: 99%

“…The enhancement of mass transport and the generation of hydrodynamic shear, which are critically important for bone development and function, depend on interstitial flow (17,18). Rotating bioreactors used to cultivate porcine TMJ constructs provided convective flow around graft surfaces but not in the graft interior (7). Bioreactors with perfusion through the cultured constructs have been preferred for engineering bone (19)(20)(21)(22), because they can provide microenvironmental control and biophysical stimulation of the cells in large constructs.…”

mentioning

confidence: 99%

Engineering anatomically shaped human bone grafts

Grayson

Fröhlich

Yeager

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

374

317

View full text Add to dashboard Cite

The ability to engineer anatomically correct pieces of viable and functional human bone would have tremendous potential for bone reconstructions after congenital defects, cancer resections, and trauma. We report that clinically sized, anatomically shaped, viable human bone grafts can be engineered by using human mesenchymal stem cells (hMSCs) and a "biomimetic" scaffold-bioreactor system. We selected the temporomandibular joint (TMJ) condylar bone as our tissue model, because of its clinical importance and the challenges associated with its complex shape. Anatomically shaped scaffolds were generated from fully decellularized trabecular bone by using digitized clinical images, seeded with hMSCs, and cultured with interstitial flow of culture medium. A bioreactor with a chamber in the exact shape of a human TMJ was designed for controllable perfusion throughout the engineered construct. By 5 weeks of cultivation, tissue growth was evidenced by the formation of confluent layers of lamellar bone (by scanning electron microscopy), markedly increased volume of mineralized matrix (by quantitative microcomputer tomography), and the formation of osteoids (histologically). Within bone grafts of this size and complexity cells were fully viable at a physiologic density, likely an important factor of graft function. Moreover, the density and architecture of bone matrix correlated with the intensity and pattern of the interstitial flow, as determined in experimental and modeling studies. This approach has potential to overcome a critical hurdle-in vitro cultivation of viable bone grafts of complex geometries-to provide patient-specific bone grafts for craniofacial and orthopedic reconstructions.biomimetic | bioreactor | craniofacial regeneration | mesenchymal stem cells | temporomandibular joint | tissue engineering

show abstract

Reconstruction of mandibular defects with autologous tissue-engineered bone

Cited by 129 publications

References 18 publications

Migration of culture-expanded human mesenchymal stem cells through bone marrow endothelium is regulated by matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-3

Migration of culture-expanded human mesenchymal stem cells through bone marrow endothelium is regulated by matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-3

Polímeros bioreabsorvíveis na engenharia de tecidos

Engineering anatomically shaped human bone grafts

Contact Info

Product

Resources

About