Microcharcoal in soils and sediments is an ideal proxy for studying fire activity. Phytoliths in soils and sediments record the environmental conditions in which the phytoliths were formed by plants. However, our understanding of the relationships between fire activity, plant communities, and the preservation of microcharcoal and phytoliths in soils and sediments remains limited. In this study, we collected soils and sediments across a gradient of burned and unburned forest in southwest China, and analyzed the microcharcoals and phytoliths in these samples to understand the relationships between these microfossils (ratios of microcharcoal to phytolith particles (Ch/Ph)), fire activity, and vegetation cover. We show that the Ch/Ph ratios recorded fire activity and were significantly different across the gradient of burned to unburned forest. The highest and lowest ratios (0.25 and 0.01) were found in burned forest (Bs1) and unburned forest samples (Us2), respectively. The ratios gradually decreased with increasing distance from the fire. This study suggests the ratio (Ch/Ph) to be a useful proxy for studying fire activity and/or history using soils and sediments.