Reconstitution of Cell Membrane Structure in vitro and its Transformation into an Excitable System

Müeller, Paul; Rudin, Donald O.; Tien, H. Ti; Wescott, William C.

doi:10.1038/194979a0

Cited by 1,303 publications

(606 citation statements)

References 5 publications

Supporting

Mentioning

592

Contrasting

Unclassified

Order By: Relevance

“…Details of preparing planar lipid bilayers is found else where 54,55 . Briefly, DPhPC (Avanti) lipid, dissolved at 10 mg ml − 1 in chloroform, was dried under a stream of nitrogen for a few minutes.…”

Section: Methodsmentioning

confidence: 99%

Self-assembling subnanometer pores with unusual mass-transport properties

et al. 2012

View full text Add to dashboard Cite

A long-standing aim in molecular self-assembly is the development of synthetic nanopores capable of mimicking the mass-transport characteristics of biological channels and pores. Here we report a strategy for enforcing the nanotubular assembly of rigid macrocycles in both the solid state and solution based on the interplay of multiple hydrogen-bonding and aromatic π − π stacking interactions. The resultant nanotubes have modifiable surfaces and inner pores of a uniform diameter defined by the constituent macrocycles. The self-assembling hydrophobic nanopores can mediate not only highly selective transmembrane ion transport, unprecedented for a synthetic nanopore, but also highly efficient transmembrane water permeability. These results establish a solid foundation for developing synthetically accessible, robust nanostructured systems with broad applications such as reconstituted mimicry of defined functions solely achieved by biological nanostructures, molecular sensing, and the fabrication of porous materials required for water purification and molecular separations.

show abstract

Section: Methodsmentioning

confidence: 99%

Self-assembling subnanometer pores with unusual mass-transport properties

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Cholesterol was used for providing better stability of BLM. BLMs were formed according to Mueller et al (1962) on a circular aperture of a 0.7 mm diameter in a Teflon partition dividing the Teflon cup into two identical compartments with a volume of 3.5 ml each. Briefly, after accurate cleaning of the cell with detergent, washing with distilled water and drying at 80°C, the aperture was pre-treated with a small amount of lipid solution (approx.…”

Section: Chemicals Preparation Of Blmmentioning

confidence: 99%

The physical properties of lipid monolayers and bilayers containing calixarenes sensitive to cytochrome c

Vitovič

Subjaková²,

Hianik³

2013

gpb

View full text Add to dashboard Cite

Abstract. We studied physical properties of the monolayers and bilayer lipid membranes (BLM) formed by calix [6]arene carboxylic acid derivative (CX) and its mixtures with diphytanoylphosphatidylcholine (DPhPC) by means of measurement surface pressure, surface dipole potential and electrostriction. CX forms stable monolayers at an air-water interface and complexes in mixed monolayers contained DPhPC. Calixarenes increase the elastic moduli of lipid monolayers as well as BLM. Cytochrome c (cyt c) specifically binds to CX by incorporation of the amino groups of lysine residues at the protein surface. This binding affected the physical properties of CX monolayers depending on their initial surface pressure. Addition of cyt c into the water subphase induced increase of surface pressure of CX monolayers at relatively low initial pressure (15 mN/m) when monolayer was in liquid expanded state (LE). This may be due to interaction of positively charged cyt c with negatively charged carboxylic groups of CX and also by its penetration into the air-water interface. However, much subtle changes were observed for higher initial surface pressure (20 and 35 mN/m) when monolayer is in liquid condensed (LC) and solid (S) state, respectively. Lysine induced substantially lower changes in surface pressure in comparison with that of cyt c.

show abstract

“…Although patch clamp is considered the gold standard, it requires a significant investment in training and equipment. Artificial lipid bilayers are an alternative platform for ion channel studies [2][3][4]. Ion channels reconstituted into artificial bilayers can be electrically measured similarly to patch clamp, while in contrast to patch clamp the lipid and surrounding solution can be controlled as well.…”

mentioning

confidence: 99%

“…In this system, lipids are dissolved in a high freezing point solvent such as hexadecane (T f = 18°C) and spread over an orifice in a hydrophobic plastic such as Teflon or Delrin (similar to standard methods [3,9]). Upon cooling the deposited lipid solution to 4°C before bilayer self-assembly is completed, it freezes, arresting the bilayer formation process.…”

mentioning

confidence: 99%

Automatable production of shippable bilayer chips by pin tool deposition for an ion channel measurement platform

Poulos¹,

Jeon

Schmidt

2010

Biotechnology Journal

View full text Add to dashboard Cite

As a step towards an automated and operator-free ion channel measurement platform we have previously demonstrated a solution formulation for artificial lipid bilayers that enabled the indefinite storage and shipping of frozen bilayer precursors. In this work, the solutions were deposited by hand. Here, we have adapted pin tools to deposit the bilayer precursor solutions onto multielement arrays, a popular method for microarray solution deposition. The pin tools have enabled the deposited volume to be applied highly repeatably and controllably, resulting in reduction of bilayer formation times to <1 h. The pin tools are also compatible with computerized motion control platforms, enabling automated and high throughput production. We discuss these results and the prospects of this technology to produce high density bilayer arrays for high throughput measurement of ion channels incorporated into artificial bilayers.

show abstract

Reconstitution of Cell Membrane Structure in vitro and its Transformation into an Excitable System

Cited by 1,303 publications

References 5 publications

Self-assembling subnanometer pores with unusual mass-transport properties

Self-assembling subnanometer pores with unusual mass-transport properties

The physical properties of lipid monolayers and bilayers containing calixarenes sensitive to cytochrome c

Automatable production of shippable bilayer chips by pin tool deposition for an ion channel measurement platform

Contact Info

Product

Resources

About