Automatable production of shippable bilayer chips by pin tool deposition for an ion channel measurement platform

Poulos, Jason L.; Jeon, Tae‐Joon; Schmidt, Jacob J.

doi:10.1002/biot.200900297

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…In the last few decades, much attention has been devoted to shedding light on the functions and mechanisms of cell membranes. In particular, creating biomimetic membrane systems integrated with functional proteins has been of great interest in relation to physiological studies, drug screening, and sensing platforms [1–5]. …”

Section: Introductionmentioning

confidence: 99%

Synthetic Biomimetic Membranes and Their Sensor Applications

Kim

Jung

Ryu

et al. 2012

Sensors

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Synthetic biomimetic membranes provide biological environments to membrane proteins. By exploiting the central roles of biological membranes, it is possible to devise biosensors, drug delivery systems, and nanocontainers using a biomimetic membrane system integrated with functional proteins. Biomimetic membranes can be created with synthetic lipids or block copolymers. These amphiphilic lipids and polymers self-assemble in an aqueous solution either into planar membranes or into vesicles. Using various techniques developed to date, both planar membranes and vesicles can provide versatile and robust platforms for a number of applications. In particular, biomimetic membranes with modified lipids or functional proteins are promising platforms for biosensors. We review recent technologies used to create synthetic biomimetic membranes and their engineered sensors applications.

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Section: Introductionmentioning

confidence: 99%

Synthetic Biomimetic Membranes and Their Sensor Applications

Kim

Jung

Ryu

et al. 2012

Sensors

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“…However, membrane precursors deposited on a thin film had a wide range of membrane formation time from 30 min to 24 h and the success rate was low. Later work with a pin tool further reduced the membrane formation time and improved the success rate by precise volume control [25].…”

Section: Introductionmentioning

confidence: 99%

Storable droplet interface lipid bilayers for cell-free ion channel studies

Jung

Choi

Kim

et al. 2011

Bioprocess Biosyst Eng

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An artificially created lipid bilayer is an important platform in studying ion channels and engineered biosensor applications. However, a lipid bilayer created using conventional techniques is fragile and short-lived, and the measurement of ion channels requires expertise and laborious procedures, precluding practical applications. Here, we demonstrate a storable droplet lipid bilayer precursor frozen with ion channels, resulting in a droplet interface bilayer upon thawing. A small vial with an aqueous droplet in organic solution was flash frozen in -80 °C methanol immediately after an aqueous droplet was introduced into the organic solution and gravity draws the droplet down to the interface upon thawing. A lipid bilayer created along the interface using this method had giga-ohm resistance and typical specific capacitance values. The noise level of this system is favorably comparable to the conventional system. The subsequent incorporation of ion channels, alpha-hemolysin and gramicidin A, showed typical conductance values consistent with those in previous literatures. This novel system to create a lipid bilayer as a whole can be automated from its manufacture to use and indefinitely stored when frozen. As a result, ion channel measurements can be carried out in any place, increasing the accessibility of ion channel studies as well as a number of applications, such as biosensors, ion channel drug screening, and biophysical studies.

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