Reconfigurable Three‐Dimensional DNA Nanostructures for the Construction of Intracellular Logic Sensors

Pei, Hao; Liang, Le; Yao, Guangbao; Li, Jiang; Huang, Qing; Fan, Chunhai

doi:10.1002/anie.201202356

Cited by 353 publications

(241 citation statements)

References 54 publications

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“…[2] Because of the endogenous nature and high programmability of DNA, self-assembled DNA nanotubes have attracted intense interest. [3] These DNA nanotubes, when appropriately modified, can be readily inserted into membranes [4] to function as biomimetic channels. Molecular dynamics (MD) simulations have also revealed interesting properties, [5] including ion flow and gating-like behaviors in these DNA-based nanochannels.…”

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confidence: 99%

Charge Neutralization Drives the Shape Reconfiguration of DNA Nanotubes

Zhao

Liu

et al. 2018

Angew Chem Int Ed

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Reconfiguration of membrane protein channels for gated transport is highly regulated under physiological conditions. However, a mechanistic understanding of such channels remains challenging owing to the difficulty in probing subtle gating-associated structural changes. Herein, we show that charge neutralization can drive the shape reconfiguration of a biomimetic 6-helix bundle DNA nanotube (6HB). Specifically, 6HB adopts a compact state when its charge is neutralized by Mg2+; whereas Na+ switches it to the expanded state, as revealed by MD simulations, small-angle X-ray scattering (SAXS), and FRET characterization. Furthermore, partial neutralization of the DNA backbone charges by chemical modification renders 6HB compact and insensitive to ions, suggesting an interplay between electrostatic and hydrophobic forces in the channels. This system provides a platform for understanding the structure-function relationship of biological channels and designing rules for the shape control of DNA nanostructures in biomedical applications.

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confidence: 99%

Charge Neutralization Drives the Shape Reconfiguration of DNA Nanotubes

Zhao

Liu

et al. 2018

Angew Chem Int Ed

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“…In their work, they exploited the DNA-driven self-assembly process [68][69] and constructed NP pyramids by locating four different NPs in the corners of the tetrahedral DNA nanostructure, including different sized AuNPs (10, 15, and 25 nm), CdSe@ZnS quantum dots (5 nm), and AgNPs (10 nm). They observed three different CD signals corresponding to the plasmonic oscillations of Au, Ag and bandgap transitions of quantum dots, which can also be tuned in the ultraviolet and visible range (350-550 nm).…”

Section: D Nanostructurementioning

confidence: 99%

Design and Fabrication of Plasmonic Nanostructures with DNA for Surface‐Enhanced Raman Spectroscopy Applications

et al. 2016

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Plasmonic nanostructures display unique and strongly enhanced optical properties, therefore hold great promise for a wide range of spectroscopic applications, particularly surface-enhanced Raman spectroscopy (SERS). It is well acknowledged that the major contributions to SERS arise from molecules positioned in nanojunctions where the optical field is intensively concentrated due to localized surface plasmon excitations. One of the key challenges in SERS therefore lies in the design and fabrication of plasmonic nanostructures with controllable nanojunctions. In recent years, by exploiting the unparalleled base-pairing self-recognition properties, DNA-mediated assembly has emerged as a powerful and programmable tool for the accurate construction of complex and hierarchical plasmonic nanostructures with well-defined geometry and topology. In this review, we will summarize recent advances on design and fabrication of a rich variety of plasmonic nanostructures by virtue of DNA nanotechnology, and discuss their optical properties as well as applications in SERS.

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“…[60] In addition, a different report showed that a series of logic gates could be constructed on the tetrahedral cage by introducing reconfigurable DNA structures such as the i-motif, and used for intracellular sensing of various targets including mercury ions, small molecules like ATP, and short strands of DNA. [61] The intracellular logic gates used fluorescence as the output signal, making it a fast and sensitive detection system. Abnormal ATP levels are observed in some metabolic diseases such as metabolic myopathies, [62] and elevated mercury levels indicate mercury poisoning or a possible autism diagnosis.…”

Section: Functionalized Dna Nanostructures For Disease Diagnosismentioning

confidence: 99%

Functionalized DNA Nanostructures for Nanomedicine

Liu

Yan

2013

Israel Journal of Chemistry

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DNA nanotechnology utilizes synthetic DNA strands as the building material to construct nanoscale devices, and the field has developed rapidly over the past decade. Recently, the use of DNA nanostructures for various applications, particularly biomedical ones, has drawn great interest. This review focuses on the most recent research directed at utilizing functionalized DNA devices for nanomedical applications and presents representative research progress in disease diagnosis, treatment and prevention. In addition, the safety and future clinical applications of DNA nanostructures are discussed.

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Reconfigurable Three‐Dimensional DNA Nanostructures for the Construction of Intracellular Logic Sensors

Cited by 353 publications

References 54 publications

Charge Neutralization Drives the Shape Reconfiguration of DNA Nanotubes

Charge Neutralization Drives the Shape Reconfiguration of DNA Nanotubes

Design and Fabrication of Plasmonic Nanostructures with DNA for Surface‐Enhanced Raman Spectroscopy Applications

Functionalized DNA Nanostructures for Nanomedicine

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