Recommended Methods for the Determination of Four Enzymes in Blood

Keiding, R.; Hørder, Mogens; Denmark, W. Gerhardt; Pitkänen, E.; Tenhunen, Raimo; Strømme, J H; Theodorsen, L.; Waldenström, J; Tryding, Nils; Westlund, L.

doi:10.3109/00365517409082499

Cited by 766 publications

(12 citation statements)

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“…Serum levels of 25-hydroxyvitamin D [s-25(OH)D], osteocalcin and bone specific alkaline phosphatase (BAP) were measured using standard procedures [18][19][20].…”

Section: Biochemical Markersmentioning

confidence: 99%

Effects of COLIA1 polymorphisms and haplotypes on perimenopausal bone mass, postmenopausal bone loss and fracture risk

et al. 2010

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“…Serum levels of 25-hydroxyvitamin D [s-25(OH)D], osteocalcin and bone specific alkaline phosphatase (BAP) were measured using standard procedures [18][19][20].…”

Section: Biochemical Markersmentioning

confidence: 99%

Effects of COLIA1 polymorphisms and haplotypes on perimenopausal bone mass, postmenopausal bone loss and fracture risk

et al. 2010

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“…Lactate dehydrogenase (lactate : NAD* oxidoreductase EC 1.1.1.27) and creatine kinase (ATP : creatine N-phosphotransferase EC 2.7.3.2) were measured spectrophotometrically at 37 °C (8,9). The measurements were done using a Perkin-Elmer spectrophotometer.…”

Section: Enzymesmentioning

confidence: 99%

Age-Dependent Variations of Lactate Dehydrogenase and Creatine Kinase Activities in Water Buffalo Calf Serum

Avallone

Lombardi²,

Florio³

et al. 1996

CCLM

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The electrophoretic patterns of the serum enzymes lactate dehydrogenase and creatine kinase from water buffalo calves are described. Differences in total activities as well as their relative distribution were seen at ages ranging from 1 to 10 weeks. While total lactate dehydrogenase activity increased by over 100%, total creatine kinase increased by almost 400%. The relative activities of lactate dehydrogenase 1 and 5 decreased with age. Lactate dehydrogenase 2 and 3 increased and lactate dehydrogenase 4 did not change.In relation to creatine kinase, the prevalent isoenzyme was creatine kinase-MM, but it's relative activity gradually decreased in comparison to the other two isoenzymes (creatine kinase-MB and creatine kinase-BB). Creatine kinase-BB was completely absent until the 3rd week of age. The percentage modifications of creatine kinase isoenzymes were correlated to age.The results suggest that isoenzymatic separation and characterization of lactate dehydrogenase and creatine kinase in relation to the various tissues can significantly contribute to the diagnosis of diseases which are linked to tissue damage.

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“…Blood samples were drawn by means of a capillary tube from eyeballs, and serum fasting insulin level was measured in all groups using the enzyme-linked immunosorbent assay. Also, serum levels of urea and creatinine were determined using an autoanalyzer (Hitachi 912 Auto-Analyzer; Hitachi, Germany) [23]. Rats were sacrificed after 8 weeks and the abdominal wall was opened.…”

Section: Seriological Studymentioning

confidence: 99%

Effect of induced diabetes mellitus on the histological structure of the renal cortex of adult male albino rats and the possible protective role of licorice extract

Shehata

Elghonaimy

Selim

et al. 2014

The Egyptian Journal of Histology

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Forty adult male albino rats were utilized. They were classified into three main groups: the control group (group I), the experimental diabetic group (group II), and the possible protected group (group III). Type 2 diabetes was induced in rats in groups II and III by giving them a high-fat diet and a single low dose of streptozotocin. Diabetic rats were divided into two subgroups: untreated subgroup IIa and treated subgroup IIb. The possible protected group received licorice ethanolic extract concomitant with the high-fat diet and the single low dose of streptozotocin. At the end of the experiment, the kidneys were dissected out and processed for light and electron microscopic examination. Fasting blood glucose level, fasting insulin level, serum urea, and creatinine were estimated and statistically analyzed. ResultsExamination of the renal cortex of untreated diabetic subgroup IIa demonstrated glomerulosclerosis and distorted podocyte foot processes. The cells lining convoluted tubules revealed thick basement membranes, disorganization of basal infoldings, and mitochondrial disarrangement. The area% of positive Bax immunoreaction was significantly increased in subgroup IIa as compared with subgroup IIb and group III. Examination of the renal cortex of the treated diabetic animals (subgroup IIb) revealed little improvement and failure of licorice extract to normalize renal cortical changes, most probably due to late intervention. In contrast, the protected group (group III) revealed a nearly preserved normal architecture. ConclusionChanges in the renal cortical structure were attenuated with prophylactic therapy of licorice ethanolic extract.

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Recommended Methods for the Determination of Four Enzymes in Blood

Cited by 766 publications

References 0 publications

Effects of COLIA1 polymorphisms and haplotypes on perimenopausal bone mass, postmenopausal bone loss and fracture risk

Effects of COLIA1 polymorphisms and haplotypes on perimenopausal bone mass, postmenopausal bone loss and fracture risk

Age-Dependent Variations of Lactate Dehydrogenase and Creatine Kinase Activities in Water Buffalo Calf Serum

Effect of induced diabetes mellitus on the histological structure of the renal cortex of adult male albino rats and the possible protective role of licorice extract

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