Recombination Within a Nucleotide-Binding-Site/Leucine-Rich-Repeat Gene Cluster Produces New Variants Conditioning Resistance to Soybean Mosaic Virus in Soybeans

Hayes, A. J.; Jeong, Soon-Chun; Gore, Michael A.; Yu, Yangyang; Buss, G. R.; Tolin, S. A.; Maroof, M. A. Saghai

doi:10.1534/genetics.166.1.493

Cited by 144 publications

(128 citation statements)

References 34 publications

(2 reference statements)

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“…For the 11 B4-CNL pseudogenes, complete ORFs could be restored after a few base-pair changes, showing that they are highly similar in regions A, B, C, and E and present from 3 (CNL-J8*) to 22 LRRs (CNL-J11*) (supplemental Figure 2B). In addition to the previously identified Co-2 bean CNL (Creusot et al 1999), the most closely related R proteins to our B4-CNL members are the soybean RPG1b CNL members exhibiting 70% of amino acid similarity with B4-CNL (Ashfield et al 2004;Hayes et al 2004).…”

Section: Resultsmentioning

confidence: 63%

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

et al. 2009

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In common bean, the B4 disease resistance (R) gene cluster is a complex cluster localized at the end of linkage group (LG) B4, containing at least three R specificities to the fungus Colletotrichum lindemuthianum. To investigate the evolution of this R cluster since the divergence of Andean and Mesoamerican gene pools, DNA sequences were characterized from two representative genotypes of the two major gene pools of common bean (BAT93: Mesoamerican; JaloEEP558: Andean). Sequences encoding 29 B4-CC nucleotide-binding-site-leucine-rich-repeat (B4-CNL) genes were determined-12 from JaloEEP558 and 17 from BAT93. Although sequence exchange events were identified, phylogenetic analyses revealed that they were not frequent enough to lead to homogenization of B4-CNL sequences within a haplotype. Genetic mapping based on pulsed-field gel electrophoresis separation confirmed that the B4-CNL family is a large family specific to one end of LG B4 and is present at two distinct blocks separated by 26 cM. Fluorescent in situ hybridization on meiotic pachytene chromosomes revealed that two B4-CNL blocks are located in the subtelomeric region of the short arm of chromosome 4 on both sides of a heterochromatic block (knob), suggesting that this peculiar genomic environment may favor the proliferation of a large R gene cluster.

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Section: Resultsmentioning

confidence: 63%

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

et al. 2009

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“…Unequal crossing-over points were found to locate primarily within intergenic regions [83,84]. Unequal crossoverdriven gene evolution has been reported in a number of other disease resistance gene families across different plant species, including the gene families at the Arabidopsis locus RPP5 [85], the soybean (Glycine max) loci Rps [86,87] and Rsv1 [88], the lettuce (Lactuca sativa) Dm3 locus [89,90], the barley (Hordeum vulgare) Hv-elF4E locus [91], and the citrus Ctv locus [69]. Similarly, unequal crossing-over takes place in gene families other than disease resistance gene families and generates novel haplotypes and alleles at those loci [70,74,75,92].…”

Section: Unequal Crossing-overmentioning

confidence: 94%

Meiosis-Driven Genome Variation in Plants

Cai¹,

Xu²

2007

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Meiosis includes two successive divisions of the nucleus with one round of DNA replication and leads to the formation of gametes with half of the chromosomes of the mother cell during sexual reproduction. It provides a cytological basis for gametogenesis and inheritance in eukaryotes. Meiotic cell division is a complex and dynamic process that involves a number of molecular and cellular events, such as DNA and chromosome replication, chromosome pairing, synapsis and recombination, chromosome segregation, and cytokinesis. Meiosis maintains genome stability and integrity over sexual life cycles. On the other hand, meiosis generates genome variations in several ways. Variant meiotic recombination resulting from specific genome structures induces deletions, duplications, and other rearrangements within the genic and non-genic genomic regions and has been considered a major driving force for gene and genome evolution in nature. Meiotic abnormalities in chromosome segregation lead to chromosomally imbalanced gametes and aneuploidy. Meiotic restitution due to failure of the first or second meiotic division gives rise to unreduced gametes, which triggers polyploidization and genome expansion. This paper reviews research regarding meiosis-driven genome variation, including deletion and duplication of genomic regions, aneuploidy, and polyploidization, and discusses the effect of related meiotic events on genome variation and evolution in plants. Knowledge of various meiosis-driven genome variations provides insight into genome evolution and genetic variability in plants and facilitates plant genome research.

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“…In either case, the NITRs present a compelling case for parallel evolution of BCR-and TCR-type V-region diversity in the context of innate immunity. [94][95][96][97][98] . The LRR-encoding cDNAs in lampreys are encoded at a single locus.…”

Section: Nk and Nk-type Receptors In Jawed Vertebratesmentioning

confidence: 99%

“…Subtracted cDNAs recovered from these cell cultures are highly enriched in sequences encoding a particularly complex set of LEUCINE-RICH REPEAT (LRR)-containing extracellular molecules. Notably, LRRs are a core feature of markedly diverse forms of pattern-recognition molecule that mediate receptor-ligand interactions throughout phylo geny [94][95][96][97][98] . The LRR-encoding cDNAs in lampreys are encoded at a single locus.…”

Section: Anticipatory Immunity In Jawless Vertebratesmentioning

confidence: 99%

Reconstructing immune phylogeny: new perspectives

2005

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Numerous studies of the mammalian immune system have begun to uncover profound interrelationships, as well as fundamental differences, between the adaptive and innate systems of immune recognition. Coincident with these investigations, the increasing experimental accessibility of non-mammalian jawed vertebrates, jawless vertebrates, protochordates and invertebrates has provided intriguing new information regarding the likely patterns of emergence of immune-related molecules during metazoan phylogeny, as well as the evolution of alternative mechanisms for receptor diversification. Such findings blur traditional distinctions between adaptive and innate immunity and emphasize that, throughout evolution, the immune system has used a remarkably extensive variety of solutions to meet fundamentally similar requirements for host protection.The evolutionary development of the METAZOANS was associated with the diversification of a wide range of specialized cell-surface molecules that mediate key metabolic processes, as well as provide crucial contact interfaces and carry out a broad range of other essential functions. It is not unexpected that some of these molecules also came to function as barriers to pathogenic invasion and, in doing so, began to carry out dedicated innate immune protective functions. Whereas the simplest form of protection, barrier formation, is essentially mechanical in nature, relentless pressure from genetic variation in pathogens probably drove the evolution of such innate immune protective molecules towards diversification and, in parallel, towards integration of signalling pathways to regulate cellular responses to external stimulation. However, despite the sophistication that such innate immune mediators achieved over time, their biological complexity, by definition, would be limited by genome space, so with increasing complexity of body plan and/or increasing pathogen sophistication, they could be overwhelmed. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author ManuscriptMore than 500 million years ago, a TRANSPOSITION event, probably involving a recombinationactivating gene (RAG)-bearing element, might have given rise to the predecessors of the rearranging antigen-binding receptors of the jawed vertebrates, which encompass the vertebrate radiations that extend from the cartilaginous fish through to humans. This is considered the defining point in the emergence of RAG-mediated (conventional) adaptive immunity 1,2 , which has evolved to create a mechanism for deriving almost limitless variation from very few genes. Studies in traditional and non-traditional animal models, such as sharks, bony fish and birds, have brought this event and its ramifications for host defence into sharper focus. We can now predict much about how these rearranging antigenbinding receptors probably arose, what alternative pathways of immune-receptor gene evolution have occurred, what relationships exist between B-and T-cell-mediated immunity and natural killer (NK)-cell function, how complex immune ...

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Recombination Within a Nucleotide-Binding-Site/Leucine-Rich-Repeat Gene Cluster Produces New Variants Conditioning Resistance to Soybean Mosaic Virus in Soybeans

Cited by 144 publications

References 34 publications

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site–Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris

Meiosis-Driven Genome Variation in Plants

Reconstructing immune phylogeny: new perspectives

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