Recombinant Synechococcus elongatus PCC 7942 for improved zeaxanthin production under natural light conditions

Sarnaik, Aditya; Nambissan, Vishnu; Pandit, Reena; Lali, Arvind

doi:10.1016/j.algal.2018.10.021

Cited by 45 publications

(14 citation statements)

References 34 publications

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“…This would allow us to obtain genetically modified species of microalgae with ameliorated or decreased enzymatic activities and able to accumulate the desired pigments. Recently, in 2018, Sarnaik et al introduced the exogenous β-carotene oxygenase gene (CrtR) from the strain Synechoccocus elongatus (PCC 7002), to prolong carotenoid pathway toward zeaxanthin production in vivo [110]. Associating the CrtR gene with the insertion of the galactose transporter from Escherichia coli led to respective zeaxanthin yields of 9 mg/g DW and 8 mg/g DW in autotrophic and mixotrophic conditions [110].…”

Section: Recent Applications In Metabolic Engineering For Xanthophylls Productionmentioning

confidence: 99%

“…Recently, in 2018, Sarnaik et al introduced the exogenous β-carotene oxygenase gene (CrtR) from the strain Synechoccocus elongatus (PCC 7002), to prolong carotenoid pathway toward zeaxanthin production in vivo [110]. Associating the CrtR gene with the insertion of the galactose transporter from Escherichia coli led to respective zeaxanthin yields of 9 mg/g DW and 8 mg/g DW in autotrophic and mixotrophic conditions [110]. ASX is commercially produced, generally for its human health benefits, and is used as an anti-inflammatory compound and as a skin protector.…”

Section: Recent Applications In Metabolic Engineering For Xanthophylls Productionmentioning

confidence: 99%

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Microalgae Xanthophylls: From Biosynthesis Pathway and Production Techniques to Encapsulation Development

Smaoui

Barkallah

Hlima

et al. 2021

Foods

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In the last 20 years, xanthophylls from microalgae have gained increased scientific and industrial interests. This review highlights the essential issues that concern this class of high value compounds. Firstly, their chemical diversity as the producer microorganisms was detailed. Then, the use of conventional and innovative extraction techniques was discussed. Upgraded knowledge on the biosynthetic pathway of the main xanthophylls produced by photosynthetic microorganisms was reviewed in depth, providing new insightful ideas, clarifying the function of these active biomolecules. In addition, the recent advances in encapsulation techniques of astaxanthin and fucoxanthin, such as spray and freeze drying, gelation, emulsification and coacervation were updated. Providing information about these topics and their applications and advances could be a help to students and young researchers who are interested in chemical and metabolic engineering, chemistry and natural products communities to approach the complex thematic of xanthophylls.

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Section: Recent Applications In Metabolic Engineering For Xanthophylls Productionmentioning

confidence: 99%

Section: Recent Applications In Metabolic Engineering For Xanthophylls Productionmentioning

confidence: 99%

Microalgae Xanthophylls: From Biosynthesis Pathway and Production Techniques to Encapsulation Development

Smaoui

Barkallah

Hlima

et al. 2021

Foods

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“…We initially established a transformation protocol for Phormidium lacuna strains HE10JO and HE10DO by electroporation that was based on protocols for other filamentous cyanobacteria [39][40][41][42]. For integration into cyanobacterial DNA we used homologous recombination, which works efficiently in diverse cyanobacteria such as Synechocystis PCC6803, Synechococcus elongatus PCC7942, or Nostoc Os-1 [43][44][45]. In the transformation vectors, the homologous sequence "sc_7_37" was interrupted by a kanR resistance cassette.…”

Section: Transformation Of Phormidium Lacunamentioning

confidence: 99%

Natural transformation of the filamentous cyanobacterium Phormidium lacuna

Nies¹,

Mielke²,

Pochert³

et al. 2020

PLoS ONE

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Research for biotechnological applications of cyanobacteria focuses on synthetic pathways and bioreactor design, while little effort is devoted to introduce new, promising organisms in the field. Applications are most often based on recombinant work, and the establishment of transformation can be a risky, time-consuming procedure. In this work we demonstrate the natural transformation of the filamentous cyanobacterium Phormidium lacuna and insertion of a selection marker into the genome by homologous recombination. This is the first example for natural transformation filamentous non-heterocystous cyanobacterium. We found that Phormidium lacuna is polyploid, each cell has about 20-90 chromosomes. Transformed filaments were resistant against up to 14 mg/ml of kanamycin. Formerly, natural transformation in cyanobacteria has been considered a rare and exclusive feature of a few unicellular species. Our finding suggests that natural competence is more distributed among cyanobacteria than previously thought. This is supported by bioinformatic analyses which show that all protein factors for natural transformation are present in the majority of the analyzed cyanobacteria.

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“…Besides, cyanobacterial ux network shows limitations to direct uxes towards acetyl-CoA and the TCA cycle, which restrain their phototrophic production of broad scope molecules such as biofuels and pharmaceutical products [2][3][4][5] . Therefore, researchers have proposed microbial co-culture formats of autotrophic and heterotrophic species that would leverage S. elongatus 2973 for its sucrose-secreting capability to provide the carbon feedstock for heterotrophic synthesis of high value products 6, 7 .…”

Section: Introductionmentioning

confidence: 99%

Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels

Zhao¹,

Sengupta

Tan³

et al. 2022

Preprint

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Synechococcus elongatus UTEX 2973 can use light and CO2 to produce sucrose, making them promising candidates to construct cocultures with heterotrophic workhorses. This envisioned process is, however, challenging to implement because of photosynthetic oxidative stress, light shading effect by heterotrophic cells, degradation of light sensitive metabolites, and high cost to separate intracellular products. Here, we demonstrated an effective ecosystem, where the sucrose producing cyanobacterium was freely grown in photo-bioreactors (PBRs), while an engineered heterotrophic workhorse (β-carotene producing Yarrowia lipolytica or indigoidine producing Pseudomonas putida) was encapsulated in calcium-alginate hydrogel beads and then placed inside the PBRs. The compartmentalization by hydrogels prevented growth interference so that the cyanobacterial culture could reach high sucrose concentrations, resulting the production of indigoidine (7.5g/L hydrogel) and β-carotene (1.3g/L hydrogel), respectively (i.e., the titers were 15 ~ 22 folds higher than that in the free cell coculture). Moreover, 13C-metabolic analysis indicated that hydrogels provided a favorable microenvironment so that the flux network of cells inside hydrogel was similar to the free culture. Finally, this novel system allowed the heterotroph- containing hydrogel beads to be easily harvested and dissolved by an EDTA solution for product and cell recovery, while the cyanobacterial culture could be continuously used for growing the next batch of immobilized workhorse heterotrophs.

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Recombinant Synechococcus elongatus PCC 7942 for improved zeaxanthin production under natural light conditions

Cited by 45 publications

References 34 publications

Microalgae Xanthophylls: From Biosynthesis Pathway and Production Techniques to Encapsulation Development

Microalgae Xanthophylls: From Biosynthesis Pathway and Production Techniques to Encapsulation Development

Natural transformation of the filamentous cyanobacterium Phormidium lacuna

Photobiological production of high-value pigments via compartmentalized co-cultures using Ca-alginate hydrogels

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