Recombinant Pseudorabies Virus Usage in Vaccine Development against Swine Infectious Disease

Zhou, Mo; Abid, Muhammad Ali; Cao, Shinuo; Zhu, Shanyuan

doi:10.3390/v15020370

Cited by 5 publications

(5 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, the advantages of recombinant virus vaccines are their predictability of response and higher efficacy [ 14 ]. Among them, PRV is a good vector for developing multivalent vaccines [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, recombinant virus vaccines offer greater response predictability and higher efficacy [ 14 ]. Among them, the pseudorabies virus (PRV) is a good vector for developing multivalent vaccines [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Multivalent vaccines utilize PRV, a member of the Herpesviridae family, subfamily Alphaherpesvirinae, and genus Varicellovirus. As a vector that expresses major antigens of various swine pathogens, PRV has proven to be effective, as the expression of foreign genes does not impede viral replication [ 15 ]. Pseudorabies (PR), also known as Aujeszky’s disease, is caused by PRV.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus CD2v and p54

Wei,

Liu,

et al. 2023

IJMS

View full text Add to dashboard Cite

African swine fever (ASF) leads to high mortality in domestic pigs and wild boar, and it is caused by the African swine fever virus (ASFV). Currently, no commercially available vaccine exists for its prevention in China. In this study, we engineered a pseudorabies recombinant virus (PRV) expressing ASFV CD2v and p54 proteins (PRV-∆TK-(CD2v)-∆gE-(p54)) using CRISPR/Cas9 and homologous recombination technology. PRV-∆TK-(CD2v)-∆gE-(p54) effectively delivers CD2v and p54, and it exhibits reduced virulence. Immunization with PRV-∆TK-(CD2v)-∆gE-(p54) neither induces pruritus nor causes systemic infection and inflammation. Furthermore, a double knockout of the TK and gE genes eliminates the depletion of T, B, and monocytes/macrophages in the blood caused by wild-type viral infection, decreases the proliferation of granulocytes to eliminate T-cell immunosuppression from granulocytes, and enhances the ability of the immune system against PRV infection. An overexpression of CD2v and p54 proteins does not alter the characteristics of PRV-∆TK/∆gE. Moreover, PRV-∆TK-(CD2v)-∆gE-(p54) successfully induces antibody production via intramuscular (IM) vaccination and confers effective protection for vaccinated mice upon challenge. Thus, PRV-∆TK-(CD2v)-∆gE-(p54) demonstrates good immunogenicity and safety, providing highly effective protection against PRV and ASFV. It potentially represents a suitable candidate for the development of a bivalent vaccine against both PRV and ASFV infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus CD2v and p54

Wei,

Liu,

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…The UL23 protein is a thymidine kinase 9 that catalyzes the synthesis of viral DNA, participates in virus replication, and the spread of the virus in the central nervous system 10 . It has been reported that PRV lacking thymidine kinase UL23 exhibits significantly reduced virulence in infected swine 11 . Therefore, Ul23 -deficient PRV does not affect the immune response and can be used for vaccine preparation 12 .…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal regulation of Pseudorabies Virus Thymidine kinase UL23 by post-translational modification

Li,

Cao,

Zhang

et al. 2024

Preprint

View full text Add to dashboard Cite

Post-translational modification plays a significant role in the interaction between viruses and their hosts. The pseudorabies virus (PRV) is a highly contagious herpesvirus that affects the central nervous system and respiratory tract of swine. However, the role of post-translational modifications, including Ubiquitination and SUMOylation, in host and PRV interplays is poorly understood. Here we examined the SUMO modification of PRV proteins and revealed that the PRV thymidine kinase UL23 can undergo SUMO modification. Bioinformatic analysis suggested four potential modification sites for UL23. Site-directed mutagenesis indicates that SUMO modification occurs at sites K242 and K267 as the wild type localizes in the nucleus while the mutants localize in the cytoplasm. Subsequently, polyclonal antibodies against mouse derived UL23 were employed to reveal that wild type UL23 was mainly located in the nucleus during PRV infection. Co-expression of UL23 with SUMO deconjugating enzymes showed that SENP1/2 inhibited the nuclear import of UL23. Whereas SUMO modification significantly impacted the localization of UL23, it did not detectably affect its stability.

show abstract

“…A small number of human infections with PRV have also been reported in recent years [ 15 ]. In pigs, the PRV Bartha K61 strain can trigger a wide range of humoral and cellular immune responses as well as be a safe and effective multivalent vaccine backbone [ 16 ]. Bartha K61 recombining the GP5 gene of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) has been proved to reduce pathogenic lesions caused by PRRSV infection in vaccinated pigs [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Application of CRISPR/Cas9 for Rapid Genome Editing of Pseudorabies Virus and Bovine Herpesvirus-1

Yu,

Liu,

Liu

et al. 2024

Viruses

View full text Add to dashboard Cite

The CRISPR/Cas9 system is widely used to manipulate viral genomes. Although Alphaherpesvirinae genomes are large and complicated to edit, in recent years several Pseudorabies virus (PRV) mutants have been successfully generated using the CRISPR/Cas9 system. However, the application of CRISPR/Cas9 editing on another member of alpha herpesviruses, bovine herpesvirus-1 (BHV-1), is rarely reported. This paper reports a rapid and straightforward approach to manipulating herpesviruses genome using CRISPR/Cas9. The recombinant plasmids contained the left and right arm of the thymidine kinase (TK) gene of PRV or of the glycoprotein I (gI) and glycoprotein E (gE) of BHV-1. Upon the cleavage of the TK or gIgE gene by Cas9 protein, this was replaced by the enhanced green fluorescence protein (eGFP) by homologous recombination. With this approach, we generated recombinant TK-/eGFP+ PRV and gIgE-/eGFP+ BHV-1 mutants and then proceeded to characterize their biological activities in vitro and in vivo. In conclusion, we showed that alpha herpesvirus, including PRV and BHV-1, can be rapidly edited using the CRISPR/Cas9 approach paving the way to the development of animal herpesvirus vaccines.

show abstract

Recombinant Pseudorabies Virus Usage in Vaccine Development against Swine Infectious Disease

Cited by 5 publications

References 69 publications

Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus CD2v and p54

Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus CD2v and p54

Spatiotemporal regulation of Pseudorabies Virus Thymidine kinase UL23 by post-translational modification

Application of CRISPR/Cas9 for Rapid Genome Editing of Pseudorabies Virus and Bovine Herpesvirus-1

Contact Info

Product

Resources

About