Recombinant Infectious Bronchitis Coronavirus Beaudette with the Spike Protein Gene of the Pathogenic M41 Strain Remains Attenuated but Induces Protective Immunity

Hodgson, Teri; Casáis, Rosa; Dove, Brian K.; Britton, Paul; Cavanagh, D.

doi:10.1128/jvi.78.24.13804-13811.2004

Cited by 123 publications

(187 citation statements)

References 42 publications

(80 reference statements)

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“…A similar finding has been reported previously, with the titres of several IB strains decreasing in TOCs after 24 h inoculation (Hodgson et al, 2004). Conversely, qRT-PCR results showed an increase in total viral amount by 4 days p.i.…”

supporting

confidence: 73%

Genetic mutations in live infectious bronchitis vaccine viruses following single or dual in vitro infection of tracheal organ cultures

Ball

Bennett

Forrester

et al. 2016

Journal of General Virology

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Despite regular co-vaccination of two different strains of live infectious bronchitis vaccine viruses, little is known about possible mutations in these viruses following vaccination. As an alternative to chicks, this study used an in vitro infection model to identify single-nucleotide polymorphisms (SNPs) within the part-S1 gene of two live infectious bronchitis virus vaccine strains (793B and Massachusetts) following single or dual inoculation onto tracheal organ cultures. Results indicate that viral titres reduced over the duration of the study; conversely, the amount of detected infectious bronchitis virus genome increased. Results demonstrate a greater number of nonsynonymous SNPs in both vaccine strains when they are co-inoculated, compared with the single inoculations. The influence of the increased SNP and hydrophobic properties of the translated proteins on the vaccine viruses' virulence is unknown.

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supporting

confidence: 73%

Genetic mutations in live infectious bronchitis vaccine viruses following single or dual in vitro infection of tracheal organ cultures

Ball

Bennett

Forrester

et al. 2016

Journal of General Virology

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“…Thus when the S gene of the non-pathogenic Beaudette strain was replaced with that of the pathogenic M41 strain, the host range of Beaudette was altered in vitro : the virus no longer replicated in Vero cells (Casais et al ., , 2003. When this spike-swapped recombinant was inoculated (by nose and eye drop) into chickens, it was still nonpathogenic */although it did stimulate protective immunity against challenge by M41 (Hodgson et al ., 2004). In addition to the four structural proteins (S, E, M and N) that are possessed by all coronaviruses, Group 2 coronaviruses contain a fourth membrane-associated protein, the haemagglutinin-esterase protein (reviewed by Enjuanes et al ., 2000;Cavanagh, 2005).…”

Section: Structural Features Of Coronavirusesmentioning

confidence: 99%

Coronaviruses in poultry and other birds

2005

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“…This leaves us far short of understanding the molecular basis of cross-protection -or lack of itas cellular immune responses play a role in protection, and these are poorly understood. We do know that the S protein alone is sufficient to induce good protective immunity [12,20,46,51,53,93,96]. There is increasing evidence that only a few amino acid differences amongst S proteins are sufficient to have a detrimental impact on cross-protection.…”

Section: Introductionmentioning

confidence: 99%

“…The development of reverse genetic ('infectious clone') systems for IBV has opened up the possibility of precisely modifying the IBV genome for vaccine development as well as for defining the roles of the virus proteins in pathogenicity -two interrelated areas of research [7,10,11,46,47,104].…”

Section: Introductionmentioning

confidence: 99%

Coronavirus avian infectious bronchitis virus

2007

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-Infectious bronchitis virus (IBV), the coronavirus of the chicken (Gallus gallus), is one of the foremost causes of economic loss within the poultry industry, affecting the performance of both meat-type and egg-laying birds. The virus replicates not only in the epithelium of upper and lower respiratory tract tissues, but also in many tissues along the alimentary tract and elsewhere e.g. kidney, oviduct and testes. It can be detected in both respiratory and faecal material. There is increasing evidence that IBV can infect species of bird other than the chicken. Interestingly breeds of chicken vary with respect to the severity of infection with IBV, which may be related to the immune response. Probably the major reason for the high profile of IBV is the existence of a very large number of serotypes. Both live and inactivated IB vaccines are used extensively, the latter requiring priming by the former. Their effectiveness is diminished by poor cross-protection. The nature of the protective immune response to IBV is poorly understood. What is known is that the surface spike protein, indeed the amino-terminal S1 half, is sufficient to induce good protective immunity. There is increasing evidence that only a few amino acid differences amongst S proteins are sufficient to have a detrimental impact on cross-protection. Experimental vector IB vaccines and genetically manipulated IBVs -with heterologous spike protein genes -have produced promising results, including in the context of in ovo vaccination.

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Recombinant Infectious Bronchitis Coronavirus Beaudette with the Spike Protein Gene of the Pathogenic M41 Strain Remains Attenuated but Induces Protective Immunity

Cited by 123 publications

References 42 publications

Genetic mutations in live infectious bronchitis vaccine viruses following single or dual in vitro infection of tracheal organ cultures

Genetic mutations in live infectious bronchitis vaccine viruses following single or dual in vitro infection of tracheal organ cultures

Coronaviruses in poultry and other birds

Coronavirus avian infectious bronchitis virus

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