Recombinant Helicoverpa armigera nucleopolyhedrovirus with arthropod‐specific neurotoxin gene RjAa17f from Rhopalurus junceus enhances the virulence against the host larvae

Yu, Huan; Zhou, Bin; Meng, Jiao; Jian, Xu; Liu, Tong‐Xian; Wang, Dun

doi:10.1111/1744-7917.12289

Cited by 9 publications

(11 citation statements)

References 43 publications

(49 reference statements)

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“…Currently, more than 240 insect virus isolates have been isolated from 196 species from 35 families and seven orders in China. Since the first viral insecticide, Helicoverpa armigera nucleopolyhedrovirus (HaNPV), was registered in 1993 [25], a total of 69 products have been authorized as commercial pesticides by the Ministry of Agriculture of China (MAC) through June 2018 [26]. In the last five years, it has been estimated that 1600 metric tons of viral insecticidal formulations were produced annually, representing nearly 0.2% of the total pesticide yield in China [27].…”

Section: Discussionmentioning

confidence: 99%

Development of large-scale mosquito densovirus production by in vivo methods

Sun

Dong

et al. 2019

Parasites Vectors

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Background Mosquito-borne diseases (MBDs) cause a significant proportion of the global infectious disease burden. Vector control remains the primary strategy available to reduce the transmission of MBDs. However, long-term, wide-scale and large-scale traditional chemical pesticide application has caused significant and increased negative effects on ecosystems and broader emerging insecticide resistance in vectors; therefore, the development of a novel alternative approach is urgently needed. Mosquito densoviruses (MDVs) are entomopathogenic viruses that exhibit a narrow host range and multiple transmission patterns, making MDVs a great potential bioinsecticide. However, the application process has been relatively stagnant over the past three decades. The major obstacle has been that viruses must be produced in mosquito cell lines; therefore, the production process is both expensive and time-consuming. Methods In our study, two wild-type (wt) MDVs, AaeDV and AalDV-3, and a recombinant rAaeDV-210 were used to infect the Aag2 and C6/36 mosquito cell lines and the 1st–2nd-instar and 3rd–4th-instar larvae of Ae. albopictus , Ae. aegypti and Cx. quinquefasciatus . Viral titers and yields in cells, media, larvae and rearing water and total viral yield were evaluated. Three kinds of virus displayed higher maximum virus titers in vivo than in vitro , and they displayed higher maximum viral yields in rearing water. Results The three viruses displayed higher total maximum viral yields in C6/36 cells than in Aag2 cells. The three viruses displayed higher total maximum viral yields in Aedes mosquitoes than in Culex mosquitoes. Higher viral yields were produced by 1st–2nd-instar larvae compared to 3rd–4th-instar larvae. The recombinant viruses did not display significantly lower yields than wt viruses in nearly all samples. In summary, by using 100 1st–2nd-instar Aedes mosquito larvae in 200 ml of rearing water, more than 10 13 genome equivalents (geq) MDV yield can be obtained. Conclusions Considering the lower production cost, this in vivo method has great potential for the large-scale production of MDVs. MDVs exhibit promising prospects and great potential for mosquito control in the future. Electronic supplementary material The online version of this article (10.1186/s13071-019-3509-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Development of large-scale mosquito densovirus production by in vivo methods

Sun

Dong

et al. 2019

Parasites Vectors

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“…Larvae of Helicoverpa armigera (Hübner) were reared on a wheat bran rich artificial diet at temperature 28 ± 1 °C, 50% relative humidity and the photoperiod of 12 L: 12 D (Light: Dark) [32]. HearNPV viral occlusion bodies (OB) were amplified in H. armigera 4th instar larvae; collected, purified, quantified and stored at 4 °C as described previously [33].…”

Section: Methodsmentioning

confidence: 99%

Effect of Different Light Spectrum in Helicoverpa armigera Larvae during HearNPV Induced Tree-Top Disease

Bhattarai

Feng

et al. 2018

Insects

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Lepidopteran larvae upon infection by baculovirus show positive photo-tactic movement during tree-top disease. In light of many insects exploiting specific spectral information for the different behavioral decision, each spectral wavelength of light is an individual parsimonious candidate for such behavior stimulation. Here, we investigated the responses of third instar Helicoverpa armigera larvae infected by Helicoverpa armigera nucleopolyhedrovirus (HearNPV) to white (broad-spectrum), blue (450–490 nm), UVA (320–400 nm), and UVB (290–320 nm) lights for the tree-top disease. Our findings suggest that tree-top phenomenon is induced only when the light is applied from above. Blue, white and UVA lights from above induced tree-top disease, causing infected larvae to die in an elevated position compared to those larvae living in the complete dark. In contrast, UVB from above did not induce tree-top disease. Blue light exerted the maximum photo-tactic response, significantly (p < 0.01) higher than white light. The magnitude of the response decreased with decreasing wavelength to UVA, and no response at UVB. Our results suggested that the spectral wavelength of the light has a significant effect on the induction of the tree-top disease in H. armigera third instar larvae infected with HearNPV.

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“…Из особенностей вирусных штаммов можно выделить узкоспецифичность, высокую вирулентность и патогенность в отношении насекомого-вредителя. Отбор и наработку биомассы штамма вируса можно осуществить только на живых насекомых или культуре клеток [41,42].…”

Section: Introductionunclassified

“…В своих исследованиях Yu H. с соавторами изучал энтомопатогенную активность штамма NPV Helicoverpa armigera на гусеницах второго-шестого возраста, заражение осуществляли в основном поверхностным методом, использовали насекомых, которые голодали перед началом экспериментальной работы в течение ночи [41]. Авторы в своих работах по оценке энтомопатогенной активности на одну гусеницу использовали 2-5 мм 2 или 1 мл среды, которую обрабатывали 3-50 мкл суспензии [44-46, 51, 55, 57].…”

Section: Introductionunclassified

Methodological Approaches to the Assessment of the Entomopathogenic Activity of Microorganisms Against Insect Pests in Vitro (Review)

Bondarchuk¹,

Tsygichko²,

Asaturova³

2021

TVAN

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Excessive application of chemical insecticides in agriculture has led to some serious problems that threaten the environment and human health. One of the possible ways to overcome the situation is to shift to environmentally-friendly preparations based on entomopathogenic bioagents. The purpose of this review was to search for and systematize methodological approaches to determine the entomopathogenic activity of microorganisms of various taxonomic groups in laboratory conditions. The expediency of choosing the necessary method when studying a particular phenomenon or process is an important point both in the fundamental and applied field of research. The primary assessment of the entomopathogenic activity of microorganisms in vitro is the basis for expanding the spectrum of their action, introducing new strains into collections. It also contributes to the replenishment of knowledge about the already known properties of various types of microorganisms. All this, certainly, is connected with the further choice of them as biological agents. Each of the presented groups of entomopathogens has its distinctive features of the mechanisms of action determined by the target insect. Concerning the choice of methodological approaches for assessing the entomopathogenic effect of fungi, the authors relied on the physiological characteristics of the insect and its stage of harmfulness. The researchers were guided by methodological approaches of oral infection of insects using an infected food source to study the activity of bacterial strains. Pathological changes in the cellular structure, as well as deformations of intestinal elements, were noted. The most common way to assess the effect of entomopathogenic viruses in the laboratory is the method of surface infection of the food source of the tested insect, taking into account the high specialization of the agent. When studying the mechanisms of action of fungal, bacterial and viral agents, researchers injected a suspension of the pathogen into the insect’s body. The search and systematization of relevant methodological approaches to assess entomopathogenic microorganisms depending on the taxonomic affiliation is an important part of the work directly related to the development of a high-quality and effective bioinsecticide.

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Recombinant Helicoverpa armigera nucleopolyhedrovirus with arthropod‐specific neurotoxin gene RjAa17f from Rhopalurus junceus enhances the virulence against the host larvae

Cited by 9 publications

References 43 publications

Development of large-scale mosquito densovirus production by in vivo methods

Development of large-scale mosquito densovirus production by in vivo methods

Effect of Different Light Spectrum in Helicoverpa armigera Larvae during HearNPV Induced Tree-Top Disease

Methodological Approaches to the Assessment of the Entomopathogenic Activity of Microorganisms Against Insect Pests in Vitro (Review)

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