Recombinant Human Acidic Fibroblast Growth Factor (aFGF) Expressed in Nicotiana benthamiana Potentially Inhibits Skin Photoaging

Abstract

Responding to the need for recombinant acidic fibroblast growth factor in the pharmaceutical and cosmetic industries, we established a scalable expression system for recombinant human aFGF using transient and a DNA replicon vector expression in . Recombinant human-acidic fibroblast growth factor was recovered following infiltration of . The optimal time point at which to harvest recombinant human acidic fibroblast growth factor expressing leaves was found to be 4 days post-infiltration, before necrosis was evident. Commassie-stained SDS-PAGE gels of His-tag column eluates, concentrated using a 10 000 molecular weight cut-off column, showed an intense band at the expected molecular weight for recombinant human acidic fibroblast growth factor. An immunoblot confirmed that this band was recombinant human acidic fibroblast growth factor. Up to 10 µg recombinant human-acidic fibroblast growth factor/g of fresh leaves were achieved by a simple affinity purification protocol using protein extract from the leaves of agroinfiltrated. The purified recombinant human acidic fibroblast growth factor improved the survival rate of UVB-irradiated HaCaT and CCD-986sk cells approximately 89 and 81 %, respectively. -derived recombinant human acidic fibroblast growth factor showed similar effects on skin cell proliferation and UVB protection compared to those of-derived recombinant human acidic fibroblast growth factor. Additionally, derived recombinant human acidic fibroblast growth factor increased type 1 procollagen synthesis up to 30 % as well as reduced UVB-induced intracellular reactive oxygen species generation in fibroblast (CCD-986sk) cells.UVB is a well-known factor that causes various types of skin damage and premature aging. Therefore, the present study demonstrated that-derived recombinant human acidic fibroblast growth factor effectively protects skin cell from UVB, suggesting its potential use as a cosmetic or therapeutic agent against skin photoaging.

A Novel Autologous Topical Serum Based on Plasma Rich in Growth Factors Technology Counteracts Ultraviolet Light-Derived Photo-Oxidative Stress

“…These results were also supported by histomorphometric analysis of irradiated 3D skin explants, where high levels of proliferating cells along with decreased apoptotic signal was observed in response to ATS treatment. This effect may be attributable to the strong mitogenic response shown by dermal cells after treatment with specific growth factors such as EGF and FGF even in the presence of UV radiation [33, 35]. In fact, after 3 days from the treatment, ATS showed to promote the proliferative activity of dermal fibroblasts up to 2.3-fold compared with irradiated cells.…”

In silico and in vivo analyses of the mutated human tissue plasminogen activator (mtPA) and the antithetical effects of P19 silencing suppressor on its expression in two Nicotiana species

“…Ha et al . (2017) interestingly showed that the expressed level of the human acidic fibroblast growth factor (aFGF) decreased approximately two-fold lower in the presence of P19 silencing inhibitor 32 . This result is in accordance with our antithetical results that the decrease of the expression level has been five-fold lower in N .…”

“…On the contrary, there are some reports that P19 does not affect the expression 30 or decreases the amount of the expression in N . benthamiana 31 , 32 . Therefore, implementing the P19 protein should be examined for each recombinant protein and cultivar independently.…”

Efficient production of human growth factors in Escherichia coli by fusing with small protein 6HFh8

“…Traditionally, epidermal GF (EGF), vascular endothelial GF (VEGF), keratinocyte GF (KGF), insulin-like GF (IGF), and broblast GF (FGF) family members are used for wound healing, curing and targeting diseases, and stimulating hair growth [3][4][5][6][7][8][9][10]. Currently, because their roles in cellular regulation in vivo include anti-wrinkle, anti-aging, anti-hair loss, and tissue repair effects, GFs are also used as cosmetic additives [11][12][13][14].…”

“…With an increasing interest in functional cosmetics, the demand for GFs has also increased. Various expression systems, including Escherichia coli [1,[15][16][17][18][19], Bacillus subtilis [20], mammalian cell [21], baculovirus [22], silkworm [23,24], and plant [11,12] systems, are used for cost-effective and e cient production of recombinant GFs. Among them, the E. coli system is frequently used for the production of recombinant proteins because of facile genetic modi cation, rapid protein expression, and high growth rate.…”