A Novel Autologous Topical Serum Based on Plasma Rich in Growth Factors Technology Counteracts Ultraviolet Light-Derived Photo-Oxidative Stress

Abstract

Introduction: Skin as the major barrier between the internal and external environments protects our body from external injuries. Ultraviolet B (UVB) radiation is majorly responsible for photoaging and is closely associated with oxidative stress, inflammation, and DNA damage. The progression in the field of biological therapies has led to the emergence of new autologous therapies based on growth factors. An autologous topical serum (ATS) based on the plasma rich in growth factors (PRGF) technology has also been developed with regenerative properties. Objective: The aim of this study was to evaluate this new topical formulation in protecting skin against UVB-induced photodamage using dermal fibroblast cultures and 3D skin models. Methods: ATS was assessed over the main mechanisms underlying photodamage including oxidative stress, cell viability, DNA damage, cell death, and biosynthetic activity. Three different irradiation protocols were tested. Results: ATS application showed to significantly reduce free radical production and cell death caused by ultraviolet radiation. It also increased cell viability and promoted the proliferative activity and fibronectin biosynthesis of dermal fibroblasts. DNA doublestrand cleavage that occurs after photo-oxidative stress was reduced. Photoexposed 3D explants showed higher levels of metabolic activity and collagen synthesis. Histomorphometric analysis also revealed a reduction in UV-derived edema, hyperkeratosis, and apoptosis and an increase in collagen and cell bioactivity. Conclusion: This preliminary study suggests that this novel ATS might counteract the harmful effects of UV radiation.

In vitro and in vivo Effect of Platelet-Rich Plasma-Based Autologous Topical Serum on Cutaneous Wound Healing

“…Results presented herein indicate that topical ES application on ulcerated models was able to maintain viable proliferating fibroblast levels and reduce both cell death and tissue damage. This could be related to previous reports that indicate the ability of ES to protect the skin from hostile microenvironments by reducing free radical accumulation and preventing DNA cleavage [29]. Enzymes like superoxide dismutase and catalase, with free radical scavenging activity, have been shown to be upregulated in response to autologous growth factors [30,31].…”

An Autologous Topical Serum Derived from Platelet-Rich Plasma Therapy for the Management of Sensitive Skin Alterations: A Case Series Report

“…In addition, ES prevented DNA cleavage that occurs after solar exposure which is directly related to a decrease in tissue necrosis and cell apoptosis. 39 Moreover, it has been suggested that PRGF mediated photo-protection may be directly related to the activation of several antioxidant enzymes and the reduction of apoptotic caspases that ultimately maintain tissue viability. 40 , 41 Thus, hyperpigmented melanin spot reduction observed herein could be an additional effect of ES when counteracting solar photo-damage symptoms.…”

Autologous Platelet Rich Plasma (PRGF) Preserves Genomic Stability of Gingival Fibroblasts and Alveolar Osteoblasts after Long-Term Cell Culture

“…This is an important factor in the safety of plasma rich in growth factors. It has also a positive outcome minimizing the effect of the oxidative stress and reactive oxygen species on the cells, for example, by reducing the DNA double-strand cleavage [ 59 ]. Furthermore, it reduces the intensity of inflammation by the inhibition of transactivation of the transcription factor kappaB and the expression of COX2 (cyclooxygenase 2) and CXCR4 C-X-C (chemokine receptor type 4) [ 18 ].…”