Autologous Platelet Rich Plasma (PRGF) Preserves Genomic Stability of Gingival Fibroblasts and Alveolar Osteoblasts after Long-Term Cell Culture

Abstract

Plasma rich in growth factors (PRGF) has several applications in dentistry that may require repeated applications of PRGF. Furthermore, it has been used for ex vivo expansion of human origin cells for their clinical application. One of the most relevant issues in these applications is to guarantee the genetic stability of cells. In this study, the chromosomal stability of gingival fibroblasts and alveolar osteoblasts after long-term culture was evaluated. Cells were expanded with PRGF or foetal bovine serum (FBS) as a culture medium supplement until passage 7 or 8 for gingival fibroblast or alveolar osteoblasts, respectively. A comparative genomic hybridization (CGH) array was used for the genetic stability study. This analysis was performed at passage 3 and after long-term culture with the corresponding culture medium supplements. The cell proliferative rate was superior after PRGF culture. Array CGH analysis of cells maintained with all the three supplements did not reveal the existence of alterations in copy number or genetic instability. The autologous PRGF technology preserves the genomic stability of cells and emerges as a safe substitute for FBS as a culture medium supplement for the clinical translation of cell therapy.

Post-Orthodontic Relapse Prevention through Administration of a Novel Synthetic Carbonated Hydroxyapatite–Chitosan Hydrogel Derived from Blood Cockle Shell (Anadara granosa L.)

“…Osteoblast activity is regulated by the secretion of calcium and phosphate ions into the adjacent tissue. Elevated concentrations of extracellular calcium impede the formation of osteoclasts while stimulating DNA synthesis and chemotaxis in osteoblastic cells [5,30]. In the interim, it has been observed that CHA induces an upregulation in the expression of transforming growth factor-β1 (TGF-β1) and bone morphogenic protein-2 (BMP-2), both of which play a significant role in the process of osteoblastogenesis.…”

Platelet-rich plasma (PRP): history of the platelets� concentrates and current applications in medicine

“…Analysis of the CGH array (Genomic Hybridization Assay) to consider chromosomal stability did not reveal genetic instability. The autologous PRGF technology, according to Anitua et al, Preserves the genomic stability of the cells and represents a valid alternative to the FBS culture medium and therefore a good supplement for cell therapy 49 .…”