Recombinant glutathione-S-transferase A3 protein regulates the angiogenesis-related genes of erythrocytes in thiram induced tibial lesions

Jahejo, Ali Raza; Rajput, Nasir Ahmed; Kashif, Jam; Kalhoro, Dildar Hussain; Niu, Sheng; Qiao, Meng-li; Ding, Zhijie; Qadir, Muhammad Farhan; Mangi, Raza Ali; Khan, Afrasyab; Ahsan, Anam; Khan, Abad; Tian, Wen‐xia

doi:10.1016/j.rvsc.2020.05.007

Cited by 15 publications

(11 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies proved that disruption of ribosome-related genes contribute to the pathogenesis ankylosing spondylitis [ 60 ]. Furthermore, previous studies proved that RPL17 was correlated to angiogenesis which is essential for the HO formation [ 61 ]. Thus the increased expression level of ribosomal proteins may indicate the initiation and formation of aberrant ectopic ossification.…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression

Wei

Guo

Wang

et al. 2022

Journal of Orthopaedic Translation

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression

Wei

Guo

Wang

et al. 2022

Journal of Orthopaedic Translation

View full text Add to dashboard Cite

“…Previously, cartilage cell damage has been reported due to the apoptosis process and abnormal protein secretion in the cartilage cells [ 17 , 18 ]. Moreover, thiram destroyed the regular columns of chondrocytes, decreasing its count, while Ex-FABP treatment enhanced that count [ 1 , 19 – 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Besides morphological analysis, it was also necessary to reveal whether the supplementation with Ex-FABP protein in thiram-induced TD chickens also affects angiogenesis and immunity-related genes. Thus, we selected 30 angiogenesis and immunity-related biomarker genes as described by Jahejo et al [ 14 , 21 ]. We used Quant StudioTM 6 6's TaKaRa SYBR Premix Ex Taq TM II (RR820A; Takara Bio Inc., Dalian, China).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasia

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Tibial dyschondroplasia (TD) is a bone disorder in which dead chondrocytes accumulate as a result of apoptosis and non-vascularization in the tibial bone of broiler chickens. The pathogenicity of TD is under extensive research but is yet not fully understood. Several studies have linked it to apoptosis and non-vascularization in the tibial growth plate (GP). We conceived the idea to find the differentially expressed genes (DEGs) in chicken erythrocytes which vary in expression over time using a likelihood-ratio test (LRT). Thiram was used to induce TD in chickens, and then injected Ex-FABP protein at 0, 20, and 50 μg.kg-1 to evaluate its therapeutic effect on 30 screened immunity and angiogenesis-related genes using quantitative PCR (qPCR). The histopathology was also performed in TD chickens to explore the shape, circularity, arrangements of chondrocytes and blood vessels. Results Clinical lameness was observed in TD chickens, which decreased with the injection of Ex-FABP. Histopathological findings support Ex-FABP as a therapeutic agent for the morphology and vascularization of affected chondrocytes in TD chickens. qPCR results of 10 immunity (TLR2, TLR3, TLR4, TLR5, TLR7, TLR15, IL-7, MyD88, MHCII, and TRAF6) and 20 angiogenesis-related genes (ITGAV, ITGA2, ITGB2, ITGB3, ITGA5, IL1R1, TBXA2R, RPL17, F13A1, CLU, RAC2, RAP1B, GIT1, FYN, IQGAP2, PTCH1, NCOR2, VAV-like, PTPN11, MAML3) regulated when Ex-FABP is injected to TD chickens. Conclusion Immunity and angiogenesis-related genes can be responsible for apoptosis of chondrocytes and vascularization in tibial GP. Injection of Ex-FABP protein to thiram induced TD chickens decrease the chondrocytes damage and improves vascularization.

show abstract

“…The bird had an obvious gait defect, which affected its ability to move about; Gait score of 4 (E) The bird had a severe gait defect; Gait score of 5 (F) The bird was incapable of sustained walking on its feet. the liver and femoral head were analyzed using ImageJ (Huang et al, 2018;Jahejo et al, 2020).…”

Section: Target Genementioning

confidence: 99%

Abnormal Lipid Profile in Fast-Growing Broilers With Spontaneous Femoral Head Necrosis

2021

View full text Add to dashboard Cite

This study investigated lipid metabolism in broilers with spontaneous femoral head necrosis (FHN) by determining the levels of markers of the blood biochemistry and bone metabolism. The birds were divided into a normal group and FHN group according to the femoral head scores of 3-, 4-, and 5-week-old chickens with FHN, and a comparative study was conducted. The study showed that spontaneous FHN broilers had a lipid metabolism disorder, hyperlipidemia, and an accumulation of lipid droplets in the femur. In addition, there were significant changes in the bone parameters and blood bone biochemistry markers, and the expression of genes related to lipid metabolism in the femoral head was also significantly increased. Therefore, FHN may result from dyslipidemia, which affects the bone growth and development of broilers.

show abstract

Recombinant glutathione-S-transferase A3 protein regulates the angiogenesis-related genes of erythrocytes in thiram induced tibial lesions

Cited by 15 publications

References 54 publications

Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression

Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression

Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasia

Abnormal Lipid Profile in Fast-Growing Broilers With Spontaneous Femoral Head Necrosis

Contact Info

Product

Resources

About