Abnormal Lipid Profile in Fast-Growing Broilers With Spontaneous Femoral Head Necrosis

Fan, Rubin; Liu, Kangping; Zhou, Zhenlei

doi:10.3389/fphys.2021.685968

Cited by 7 publications

(3 citation statements)

References 49 publications

(56 reference statements)

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“…The pathology of osteomyelitis is characterized by localized inflammation, bone mineral loss, and structural damage, which share similarities with broiler BCO ( Grbic et al, 2014 ). Furthermore, a recent study on femoral necrosis pathogenicity also reported an abnormal increased in lipid metabolism and decreases in bone formation in a chicken femoral head necrosis disease model ( Fan et al, 2021 ). Therefore, by gathering all the evidence above, we proposed that oxidative stress could potentially be a co-factor involved in chicken bone necrosis.…”

Section: Discussionmentioning

confidence: 97%

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

Tompkins¹,

Liu²,

Kim³

2023

Front. Physiol.

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The effects of hydrogen peroxide (H2O2) on the osteogenic differentiation of primary chicken mesenchymal stem cells (MSCs) were investigated. MSCs were subjected to an osteogenic program and exposed to various concentrations of H2O2 for 14 days. Results showed that high concentrations of H2O2 (200 and 400 nM) significantly increased pro-apoptotic marker CASP8 expression and impaired osteogenic differentiation, as indicated by decreased mRNA expression levels of osteogenesis-related genes and reduced in vitro mineralization. In contrast, long-term H2O2 exposure promoted basal expression of adipogenic markers at the expense of osteogenesis in MSCs during osteogenic differentiation, and increased intracellular reactive oxygen species (ROS) production, as well as altered antioxidant enzyme gene expression. These findings suggest that long-term H2O2-induced ROS production impairs osteogenic differentiation in chicken MSCs under an osteogenic program.

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Section: Discussionmentioning

confidence: 97%

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

Tompkins¹,

Liu²,

Kim³

2023

Front. Physiol.

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“…As a bone disorder, FHN is characterized by periarticular bone disintegration and cartilage degeneration [ 22 ]. According to our previous study, FHN-affected broilers showed lipid metabolism disorders, increased lipid droplet formation, an imbalance in cartilage homeostasis, reduced extracellular matrix (ECM) formation, and simultaneous apoptosis [ 23 , 24 , 25 ]. Reduced osteogenic and chondrogenic differentiation of BMSCs in FHN-affected broilers and enhanced lipogenic differentiation were observed in the present study ( Figure 1 B).…”

Section: Discussionmentioning

confidence: 99%

Notch-RBPJ Pathway for the Differentiation of Bone Marrow Mesenchymal Stem Cells in Femoral Head Necrosis

Liu

et al. 2023

IJMS

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Femoral head necrosis (FHN) is a common leg disease in broilers, resulting in economic losses in the poultry industry. The occurrence of FHN is closely related to the decrease in the number of bone marrow mesenchymal stem cells (BMSCs) and the change in differentiation direction. This study aimed to investigate the function of differentiation of BMSCs in the development of FHN. We isolated and cultured BMSCs from spontaneous FHN-affected broilers and normal broilers, assessed the ability of BMSCs into three lineages by multiple staining methods, and found that BMSCs isolated from FHN-affected broilers demonstrated enhanced lipogenic differentiation, activated Notch-RBPJ signaling pathway, and diminished osteogenic and chondrogenic differentiation. The treatment of BMSCs with methylprednisolone (MP) revealed a significant decrease in the expressions of Runx2, BMP2, Col2a1 and Aggrecan, while the expressions of p-Notch1/Notch1, Notch2 and RBPJ were increased significantly. Jagged-1 (JAG-1, Notch activator)/DAPT (γ-secretase inhibitor) could promote/inhibit the osteogenic or chondrogenic ability of MP-treated BMSCs, respectively, whereas the differentiation ability of BMSCs was restored after transfection with si-RBPJ. The above results suggest that the Notch-RBPJ pathway plays important role in FHN progression by modulating the osteogenic and chondrogenic differentiation of BMSCs.

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“…It is estimated that approximately 12.5 billion poultry worldwide suffer from various leg injuries each year [ 5 ]. There are several common bone diseases in the poultry industry, including tibial dyschondroplasia (TD), valgus–varus deformity (VVD), femoral head necrosis (FHN), and bacterial chondronecrosis with osteomyelitis (BCO) [ 2 , 3 , 6 , 7 , 8 ]. In the UK, around 27% of morbid broilers have dyskinesia, and 3.3% of them cannot move [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Probiotics and Gut Microbiota on Bone Metabolism in Chickens: A Review

Chen

Zhang

et al. 2022

Metabolites

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Broiler leg diseases are a common abnormal bone metabolism issue that leads to poor leg health in growing poultry. Bone metabolism is a complicated regulatory process controlled by genetic, nutritional, feeding management, environmental, or other influencing factors. The gut microbiota constitutes the largest micro-ecosystem in animals and is closely related to many metabolic disorders, including bone disease, by affecting the absorption of nutrients and the barrier function of the gastrointestinal tract and regulating the immune system and even the brain–gut–bone axis. Recently, probiotic-based dietary supplementation has emerged as an emerging strategy to improve bone health in chickens by regulating bone metabolism based on the gut–bone axis. This review aims to summarize the regulatory mechanisms of probiotics in the gut microbiota on bone metabolism and to provide new insights for the prevention and treatment of bone diseases in broiler chickens.

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Abnormal Lipid Profile in Fast-Growing Broilers With Spontaneous Femoral Head Necrosis

Cited by 7 publications

References 49 publications

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

Notch-RBPJ Pathway for the Differentiation of Bone Marrow Mesenchymal Stem Cells in Femoral Head Necrosis

Effects of Probiotics and Gut Microbiota on Bone Metabolism in Chickens: A Review

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