Recombinant expression of Taenia solium TS14 antigen and its utilization for immunodiagnosis of neurocysticercosis

Silva, Márcia Ramos Monteiro da; Maia, Antônio Augusto Mendes; Espíndola, Noeli Maria; Machado, Luı́s dos Ramos; Vaz, Adelaide José; Henrique-Silva, Flávio

doi:10.1016/j.actatropica.2006.10.009

Cited by 18 publications

(19 citation statements)

References 32 publications

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“…[99] The purified G-F18 fusion protein synthesized by antibody screening of T. solium metacestode cDNA library showed the best results when it was used in ELISA with sera from NCC patients. [95] In a study by Chung, et al [94] , conducted in Korea, the EITB analysis of sera using a 10 kDa recombinant protein, from patients with active NCC, showed a strong reactivity of 97%.…”

Section: Antigens Of the Cysticercimentioning

confidence: 99%

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Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Parija

Gireesh

2011

Trop Parasitol

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Neurocysticercosis (NCC) is difficult to diagnose clinically because of its varied clinical presentation. However, an accurate diagnosis is possible only after suspicion on epidemiological grounds, proper interpretation of the clinical data, analysis of the findings on imaging studies, and specific immunological tests on the serum and cerebrospinal fluid (CSF). The diagnosis of NCC by any single parameter thus continues to remain difficult. In the past, detection of NCC was based on autopsy studies and histological confirmation. In recent times, the advent of imaging methods such as computed tomography and magnetic resonance imaging have provided excellent non-invasive tools for easy detection of NCC. Nevertheless, an imaging technique of the brain, although useful, is not considered as a gold standard for the diagnosis of NCC. Serological tests are being increasingly used in adjunct with imaging techniques, to aid the diagnosis of NCC. Immunodiagnostic techniques include detection methods for specific antibodies and for circulating parasite antigens in the serum and CSF. Currently, many of the immunodiagnostic tests, including the enzyme-linked immunosorbent assay and enzyme immunotransfer blot, use purified native antigens for the immunodiagnosis of NCC. Nevertheless, the main problem with the use of native cysticercal antigens is that the native proteins often show cross reactions with sera from humans infected with other parasites. The preparation of native antigens also demand a constant supply of parasitic material from the intermediate host pig. In order to overcome the problems in using native antigens, the recombinant antigens or synthetic peptides, which can be produced under stable conditions, are being evaluated for the serodiagnosis of NCC.

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Section: Antigens Of the Cysticercimentioning

confidence: 99%

“…[83] Production of antigens by recombinant DNA technology ensures a uniform, specific, pure, and continuous supply, which can be used in the serodiagnosis of an infectious disease. [99105]…”

Section: Antigens Of the Cysticercimentioning

confidence: 99%

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Parija

Gireesh

2011

Trop Parasitol

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“…For instance, from the members of the 8 kDa family, Ts18var1 has been produced in insect cells [16] as well as TsRs1, Ts18var1, and Ts18 Var3 [20]; the 14 and 18 kDa proteins produced by recombination [27]; Ts14, Ts18var1, TSRS1, and TSRS2var1 by chemical synthesis [29], and full-length Ts18 and Ts14 by chemical ligation [19], Ag1V1/Ag2 by recombination [26] as well as Ts8B1, Ts8B2, Ts8B3 [18], Ts14 [27] and a 10 kDa protein [17, 25]; GP50, which is not a member of the 8 kDa family but it is part of the LLGPs, was produced by recombination in bacteria and in a baculovirus expression system [16, 21]. Other proteins outside those from LLGPs that have also been produced or synthesized include T24 (integral membrane protein that does not bind to lentil lectin) produced in a droshophila cell line [22]; HP6-Tsag (oncospheral adhesion protein of Taenia saginata ) in bacteria and baculovirus systems with similar specificities between the systems (93–95%), but higher sensitivity for the inactive cases by the baculovirus protein (48–64%) [95]; peptide NC-1 selected by phage-display [23]; peptides KETc12, 410, and 413 synthesized from a cDNA library of T. crassiceps [24], and recombinant TS24 and Es33 [28].…”

Section: Advances In the Methods For Inmunodiagnosis Of Ncmentioning

confidence: 99%

Immunodiagnosis of Neurocysticercosis: Ways to Focus on the Challenge

Esquivel-Velázquez¹,

Ostoa‐Saloma²,

Morales‐Montor³

et al. 2011

Journal of Biomedicine and Biotechnology

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Neurocysticercosis (NCC) is a disease of the central nervous system that is considered a public health problem in endemic areas. The definitive diagnosis of this disease is made using a combination of tools that include imaging of the brain and immunodiagnostic tests, but the facilities for performing them are usually not available in endemic areas. The immunodiagnosis of NCC is a useful tool that can provide important information on whether a patient is infected or not, but it presents many drawbacks as not all infected patients can be detected. These tests rely on purified or semipurified antigens that are sometimes difficult to prepare. Recent efforts have focused on the production of recombinant or synthetic antigens for the immunodiagnosis of NCC and interesting studies propose the use of new elements as nanobodies for diagnostic purposes. However, an immunodiagnostic test that can be considered as “gold standard” has not been developed so far. The complex nature of cysticercotic disease and the simplicity of common immunological assumptions involved explain the low scores and reproducibility of immunotests in the diagnosis of NCC. Here, the most important efforts for developing an immunodiagnostic test of NCC are listed and discussed. A more punctilious strategy based on the design of panels of confirmed positive and negative samples, the use of blind tests, and a worldwide effort is proposed in order to develop an immunodiagnostic test that can provide comparable results. The identification of a set of specific and representative antigens of T. solium and a thorough compilation of the many forms of antibody response of humans to the many forms of T. solium disease are also stressed as necessary.

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“…Esta, no antígeno HP6--GST, foi de 81% no soro e 73% no liquor, enquanto para o antígeno HP6-bac a sensibilidade foi de 77% nos dois tipos de amostras (26) . Quanto aos antígenos recombinantes de T. solium descritos, destacam-se os antígenos recombinantes de 8 kDa, Ts8B (26,28) , 10 kDa (18) , 14 kDa, TS14 (36,37,77) , 24 KDa, TS24 (39) , TS24H e domínio hidrofílico extracelular do TS24 (49) , além da gliporoteína de 50 kDa e GP50 (38) . O antígeno recombinante de 10 kDa foi produzido em E. coli e analisado pela técnica de WB com 190 amostras séricas de pacientes com neurocisticercose e 180 amostras controle, incluindo soros de indivíduos sadios e de pacientes com outras parasitoses.…”

Section: Antígeno Recombinante E Sintéticounclassified

Diagnóstico laboratorial da neurocisticercose: revisão e perspectivas

Togoro¹,

Souza²,

Sato³

2012

J. Bras. Patol. Med. Lab.

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A neurocisticercose é causada por Cysticercus cellulose, a forma larval de Taenia solium, quando este se aloja no sistema nervoso central. O seu diagnóstico é realizado com base em dados clínicos, epidemiológicos, demonstração do agente etiológico pelas técnicas de imagem e testes laboratoriais. No presente estudo, apresentamos uma revisão do diagnóstico laboratorial, com ênfase no desempenho dos testes para pesquisa de anticorpos específicos e detecção de antígenos circulantes, utilização de antígeno homólogo ou heterólogo, nativo e recombinante, bem como a aplicação de métodos moleculares. resumo unitermos Neurocisticercose Taenia solium Cysticercus cellulosaeDiagnóstico laboratorial abstract Neurocysticercosis is caused by Cysticercus cellulosae, the larval form of Taenia solium, when it lodges in the central nervous system. The diagnosis of neurocysticercosis is based on clinical and epidemiological data, neuroimaging findings of etiological agent and serologic test results. Herein we present a review of clinical diagnosis, emphasizing test performance for specific antibody and antigen detection, the use of homologous or heterologous antigen, native and recombinant antigens as well as the application of molecular methods.

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Recombinant expression of Taenia solium TS14 antigen and its utilization for immunodiagnosis of neurocysticercosis

Cited by 18 publications

References 32 publications

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Cysticercus cellulosae antigens in the serodiagnosis of neurocysticercosis

Immunodiagnosis of Neurocysticercosis: Ways to Focus on the Challenge

Diagnóstico laboratorial da neurocisticercose: revisão e perspectivas

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