Recombinant Bacillus subtilis 26DCryChS line with gene Btcry1Ia encoding Cry1Ia toxin from Bacillus thuringiensis promotes integrated wheat defense against pathogen Stagonospora nodorum Berk. and greenbug Schizaphis graminum Rond.

Максимов, И. В.; Благова, Д. К.; Веселова, С. В.; Sorokan, A. V.; Бурханова, Г. Ф.; Черепанова, Е. А.; Сарварова, Е. Р.; Rumyantsev, S. D.; Alekseev, V. Yu.; Khayrullin, R.M.

doi:10.1016/j.biocontrol.2020.104242

Cited by 30 publications

(29 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The previously obtained B. subtilis 26DCгуCһЅ strain, which was developed on the basis of the endophytic B. subtilis 26D strain, is able to inhibit the propagation of oomycete P. infestans mycelium in vitro and decreased late blight lesion formation on potato leaves as well as the original strain B. subtilis 26D ( Figure 1 and Figure 2 ). B. subtilis 26D showed proteolytic activity that can cause observed impact of B. subtilis 26DCгуCһЅ [ 13 ]. Thus, Bacillus amyloliquefaciens isolates which produce proteases display antagonistic activity against pathogenic fungi Fusarium oxysporum, Alternaria alternate , and Macrophomina phaseolina [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…This “hidden existence”, in addition, allows reducing the impact of the ambient environment on microorganisms. It has been revealed that certain PGPB strains not only stimulate both plant growth and resistance against pathogens and pests [ 10 , 11 ] but also show fungicidal [ 12 ], aphicidal [ 8 , 13 ], and insecticidal activities [ 14 ] as a consequence of synthesizing antibiotics and biosurfactants [ 15 ]. Among them, Bacillus strains are important resources for biocontrol agents development.…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that some surfactin-producing Bacillus strains can elicit ISR to Botrytis cinerea in grape, strawberry, and tomato plants [ 24 ] and Rhizoctonia solani in lettuce [ 25 ]. Recently, aphicidal and insecticidal activity of surfactin produced by B. subtilis and surfactin-producing strain B. subtilis 26D has been shown [ 13 , 14 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we obtained the novel recombinant B. subtilis 26DCryChS (RCAM04928, ARRIAM collection) strain that expresses the gene Btcry1Ia [ 29 ] encoding Cry1Ia δ-endotoxin originated from the B. thuringiensis B-5351 strain. It was reported that B. subtilis 26DCryChS can exist endophytically in wheat plants and synthesize lipopeptide surfactin as well as the source strain B. subtilis 26D and is capable of producing Cry1Ia δ-endotoxin from the donor of the Btcry1Ia gene B. thuringiensis B-5351 strain [ 13 ]. The B. subtilis 26DCryChS strain shows aphicidal activity against Schizaphis graminum as compared with both B. thuringiensis B-5351 and B. subtilis 26D strains and inhibited the growth of the wheat pathogen Stagonospora nodorum Berk.…”

Section: Introductionmentioning

confidence: 99%

“…The B. subtilis 26DCryChS strain shows aphicidal activity against Schizaphis graminum as compared with both B. thuringiensis B-5351 and B. subtilis 26D strains and inhibited the growth of the wheat pathogen Stagonospora nodorum Berk. that causes Septoria blotch [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Endophytic Strain Bacillus subtilis 26DCryChS Producing Cry1Ia Toxin from Bacillus thuringiensis Promotes Multifaceted Potato Defense against Phytophthora infestans (Mont.) de Bary and Pest Leptinotarsa decemlineata Say

Sorokan

Benkovskaya

Бурханова

et al. 2020

Plants

View full text Add to dashboard Cite

Novel properties of a previously obtained Bacillus subtilis 26DCryChS strain are described. The B. subtilis 26DCryChS strain is able to produce Cry1Ia δ-endotoxin from B. thuringiensis B-5351 and to exist in internal plant tissues of potato plants in the same manner as the endophytic B. subtilis 26D source strain (487 ± 53 and 420 ± 63 CFU*103/g, respectively). B. subtilis 26DCryChS, as much as the original B. subtilis 26D strain, inhibited mycelium growth of oomycete Phytophthora infestans (Mont.) de Bary and reduced late blight symptoms development on plants by 35% compared with non-treated ones, as well as showed insecticidal activity against Leptinotarsa decemlineata. Production of the fluorescent GFP protein in the B. subtilis 26D genome allowed visualizing the endophytes around damaged sites on beetle intestines. Bacillus strains under investigation induced systemic resistance to P. infestans and L. decemlineata through the activation of the transcription of PR genes in potato plants. Thus, the B. subtilis 26DCryChS strain was able to induce transcription of jasmonate-dependent genes and acquired the ability to promote transcription of a salicylate-dependent gene (PR1) in plants infected with the late blight agent and damaged by Colorado potato beetle larvae. The B. subtilis 26DCryChS strain could be put forward as a modern approach for biocontrol agents design.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Endophytic Strain Bacillus subtilis 26DCryChS Producing Cry1Ia Toxin from Bacillus thuringiensis Promotes Multifaceted Potato Defense against Phytophthora infestans (Mont.) de Bary and Pest Leptinotarsa decemlineata Say

Sorokan

Benkovskaya

Бурханова

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

A review of biocontrol agents in controlling late blight of potatoes and tomatoes caused by Phytophthora infestans and the underlying mechanisms

Wang

Long

2023

Pest Management Science

View full text Add to dashboard Cite

Phytophthora infestans causes light blight on potatoes and tomatoes, which has a significant economic impact on agriculture. The management of late blight has been largely dependent on the application of synthetic fungicides, which is not ultimate solution for sustainable agriculture and environmental safety. Biocontrol strategies are expected to be alternative methods to the conventional chemicals in controlling plant diseases in the integrated pest management (IPM) programs. Well‐studied biocontrol agents against P. infestans include fungi, oomycetes, bacteria, and compounds produced by these antagonists, in addition to certain bioactive metabolites produced by plants. Laboratory and greenhouse experiments suggest a potential for using biocontrol in practical late blight disease management. However, the transition of biocontrol to field applications is problematic for the moment, due to low and variable efficacies. In this review, we provide a comprehensive summary on these biocontrol strategies and the underlying corresponding mechanisms. To give a more intuitive understanding of the promising biocontrol agents against P. infestans in agricultural systems, we discuss the utilizations, modes of action and future potentials of these antagonists based on their taxonomic classifications. To achieve a goal of best possible results produced by biocontrol agents, it is suggested to work on field trials, strain modifications, formulations, regulations, and optimizations of application. Combined biocontrol agents having different modes of action or biological adaptation traits may be used to strengthen the biocontrol efficacy. More importantly, biological control agents should be applied in the coordination of other existing and forthcoming methods in the IPM programs.This article is protected by copyright. All rights reserved.

show abstract

Discovery of Bioactive Natural Products from Bacillus Species: Chemistry, Biosynthesis and Biological Activities

Surovy

Rahman

Dame

et al. 2022

Bacilli in Climate Resilient Agriculture and Bioprospecting

View full text Add to dashboard Cite

Recombinant Bacillus subtilis 26DCryChS line with gene Btcry1Ia encoding Cry1Ia toxin from Bacillus thuringiensis promotes integrated wheat defense against pathogen Stagonospora nodorum Berk. and greenbug Schizaphis graminum Rond.

Cited by 30 publications

References 58 publications

Endophytic Strain Bacillus subtilis 26DCryChS Producing Cry1Ia Toxin from Bacillus thuringiensis Promotes Multifaceted Potato Defense against Phytophthora infestans (Mont.) de Bary and Pest Leptinotarsa decemlineata Say

Endophytic Strain Bacillus subtilis 26DCryChS Producing Cry1Ia Toxin from Bacillus thuringiensis Promotes Multifaceted Potato Defense against Phytophthora infestans (Mont.) de Bary and Pest Leptinotarsa decemlineata Say

A review of biocontrol agents in controlling late blight of potatoes and tomatoes caused by Phytophthora infestans and the underlying mechanisms

Discovery of Bioactive Natural Products from Bacillus Species: Chemistry, Biosynthesis and Biological Activities

Contact Info

Product

Resources

About