Recombinant antibodies to small analytes and prospects for deriving them from synthetic combinatorial libraries

Karu, Alexander E.; Scholthof, Karen‐Beth G.; Zhang, Guisheng; Bell, Christopher W.

doi:10.1080/09540109409354839

Cited by 21 publications

(14 citation statements)

References 40 publications

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“…Thus antibody selection and production can be started directly in vitro and completed within a time span of one or two months. A first attempt was reported by Karu et al [32]. The authors utilized a heavy chain CDR3-randomized antibody library generated by the group of Richard Lerner (Scripps Institute, California) to isolate antibodies against diuron.…”

Section: Antibodies Derived From Librariesmentioning

confidence: 98%

Recombinant antibodies for environmental analysis

Kramer

Hock

2003

Analytical and Bioanalytical Chemistry

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Initial steps of antibody engineering in the late eighties revolutionized the technology of antibody production, particularly in the area of immunotherapy and diagnostics. Hallmarks that seemed to be out of reach for a long time are now the state of the art, e.g. tailoring of antibodies to match particular needs or by-passing immunization by use of antibody libraries. Despite the apparent benefits of recombinant antibody technologies, this field has been opened up hesitantly for other applications. This review addresses the development of recombinant antibody synthesis in environmental analysis. Examples are given of the molecular evolution of pesticide antibodies and their application for the analysis of real samples.

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Section: Antibodies Derived From Librariesmentioning

confidence: 98%

Recombinant antibodies for environmental analysis

Kramer

Hock

2003

Analytical and Bioanalytical Chemistry

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“…In addition to their use in human therapies, virus vectors that display single-chain antibodies also have potential to be used as sensitive reagents to detect small organic molecules in water (84). Diuron is a widely-used leachable herbicide that must be monitored by regulatory agencies for ground-and well-water contamination in areas of intensive agricultural production (84)(85)(86).…”

Section: High Value Medicinal and Diagnostic Proteinsmentioning

confidence: 99%

“…Diuron is a widely-used leachable herbicide that must be monitored by regulatory agencies for ground-and well-water contamination in areas of intensive agricultural production (84)(85)(86). Heavy-and light-chain antibody cDNAs were cloned from a diuron-specific hybridoma (MAb481) and overexpressed in E. coli as Fab481.1 (86).…”

Section: High Value Medicinal and Diagnostic Proteinsmentioning

confidence: 99%

Plant Virus Gene Vectors: Biotechnology Applications in Agriculture and Medicine

Mirkov

Scholthof

2002

Genetic Engineering

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INTRODUCTIONPlant virus vectors have been developed as a result of 20 years of advances in molecular biology, which produced infectious plasmid-based clones of RNA and DNA plant viruses and viroids that could be routinely amplified in E. coli. This work built on a century of progress in virology, much of which was based on understanding the biology of Tobacco mosaic virus (TMV) (1). With the availability of the rice and Arabidopis draft genome sequences, expressed sequence tags (ESTs) and microarrays, crystal structures of many plant viruses, and public interest in non-transgenic strategies for crop improvement, the technology of plant virus gene vectors holds much promise for the coming decade (2-4). The intent of this review is to define virus-vector strategies, describe uses for improving crop health, and point out possible beneficial overlaps with veterinary and human medicine.*To whom correspondence should be addressed.(1) Texas Agricultural Experiment Station, Weslaco, TX 78596. DEFINING THE SYSTEMSMethods to regenerate transgenic plants are widely used in plant biology and biotechnology. This involves the use of either Agrobacterium tumefaciens or biolistics (e.g., gene guns) to deliver genetically customized gene expression cassettes into the nucleus of a plant cell. In this case, the DNA becomes integrated into the chromosome and with the use of selectable markers and tissue culture procedures, the cells containing the foreign insert serve as the progenitors for regeneration of whole transgenic plants. This process usually takes at least several months, and mostly longer, to obtain plants with a stable genetic trait.Plant viruses cause harmful diseases that can result in significant reductions in crop yield and economic losses to growers. However, from a biotechnology point of view, viruses collectively can also be regarded as 'molecular tools' (2). One intriguing applicable feature of plant viruses is their capacity to serve as gene vectors. With rare exception (5, 6), plant viruses do not integrate into the plant chromosome but instead transiently express their genes upon infection (7). Because plant viruses replicate to a very high copy number in infected cells, substantial levels of gene expression can be achieved. By standard molecular biology techniques, plant viruses can be supplied with foreign genes and within a few days after inoculation, plant virus gene vectors can provide high levels of transient foreign gene expression (2,4,(8)(9)(10)(11)(12).Many proteins used for biotechnology applications are not biologically active unless they undergo specific post-translational modifications, such as glycosylation or phosphorylation, in eukaryotic cells. Plants are attractive substrates for the synthesis and purification of proteins because they can carry out these modifications and, through the ages of agricultural development, it has become fairly straightforward to grow crop plants that yield a large, uniform biomass. In many cases it is desirable to have the foreign gene expressed in transgenic p...

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“…18,[42][43][44] We recovered antibodies that bound chlorophenolic haptens, but none competitively bound the parent compound (the herbicide diuron) in solution. 45 In the present project, we first attempted to select rFabs that bound benzo[a]pyrene or fluoranthene haptens from the Fab 2LOX library described in Methods. The unsuccessful 46 We selected and enriched hapten-specific binders about 10 6 -fold from the FAB 2LOX library.…”

Section: Structural and Chemical Bases Of Pah Recognitionmentioning

confidence: 99%