Recombinant antibodies for environmental analysis

Abstract

Initial steps of antibody engineering in the late eighties revolutionized the technology of antibody production, particularly in the area of immunotherapy and diagnostics. Hallmarks that seemed to be out of reach for a long time are now the state of the art, e.g. tailoring of antibodies to match particular needs or by-passing immunization by use of antibody libraries. Despite the apparent benefits of recombinant antibody technologies, this field has been opened up hesitantly for other applications. This review addresses the development of recombinant antibody synthesis in environmental analysis. Examples are given of the molecular evolution of pesticide antibodies and their application for the analysis of real samples.

Screening antibody and immunosorbent selectivity by two‐dimensional liquid chromatography‐MS/MS (2‐D LC‐MS/MS)

“…Antibodies have played a prominent role in bioanalytical chemistry [1]. Their ability to recognize specific structural features of analytes is of enormous utility in the isolation, identification, and quantification of a broad range of proteins [2], carbohydrates [3], and haptens [4], especially when the antibody is immobilized on a chromatographic support matrix, a microtiter well, or an immunological array element.…”

Recombinant Antibodies and Non-Antibody Scaffolds for Immunoassays

“…A large number of rAbs have been used successfully to develop diagnostic kits, therapeutics and biosensors (Holliger et al, 2005;Huang et al, 2010;Kramer & Hock, 2003). The majority of the targets were large molecular weight analytes such as proteins and peptides.…”

Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices

“…Nevertheless, immunized libraries were a straightforward starting material for obtaining high affinity antibodies to low molecular weight compounds (Tout, Yau, Trevors, Lee, & Hall, 2001;Kramer & Hock, 2003).…”