Recognition of cmv pp65 protein antigen by human cd4 t-cell lines induced with an immunodominant peptide pool

Pira, Giuseppina Li; Bottone, Laura; Ivaldi, Federico; Pelizzoli, Roberta; Bracci, Luisa; Lozzi, Luisa; Scarso, Lucia; Tripodi, Gino; Manca, Fabrizio

doi:10.1016/j.humimm.2004.02.010

Cited by 10 publications

(8 citation statements)

References 37 publications

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“…This was further substantiated by demonstrating that high doses of autologous monocytes, which function as APCs for CD4 T-cell stimulation when given at appropriate concentration, can exert an inhibitory function when present in excess ( Figure 2E). This nonspecific suppressive function was also seen with fully human leukocyte antigen-mismatched monocytes, that are neither recognized as an allostimulus by our CD4 T-cell lines devoid of alloreactivity [13,14], nor contribute to antigen presentation because they lack the appropriate human leukocyte antigen restriction alleles ( Figure 2E, solid bars). The conditioned medium obtained from monocytes cultured for 2 days at the highest concentration did not contain detectable inhibitory function for antigen-driven proliferation of specific T cells (not shown).…”

Section: Hsfs and Monocytes Inhibit T-cell Proliferation Similarly Tomentioning

confidence: 75%

“…The immunodominant protein pp65 expressed in Escherichia coli was prepared and used at 5 g/ml. Peptides from pp65 were used at 1g/ml to pulse APCs [12,13]. The following peptides were used: CD4-Th peptide 30, 117-PLKMLNIPSINVHHY-131 and CD8-CTL peptide 123, 489-AGILARNLVPM-VATV-503.…”

Section: Materials and Methods Antigens And Mediamentioning

confidence: 99%

“…CD4 T-cell lines were generated from peripheral blood mononuclear cells (PBMCs) obtained from heparinized blood of healthy donors aged 35-55, by repeated stimulation of specific T cells with antigen plus interleukin-2 (IL-2), as described in detail [12,13]. PBMC donors were unrelated with BMSC donors.…”

Section: Generation Of Antigen-specific T-cell Linesmentioning

confidence: 99%

“…PBMC donors were unrelated with BMSC donors. Restimulation cycles were performed every 3 weeks on 2 ϫ 10 5 T cells [12,13]. These conditions are selective for CD4 cells.…”

Section: Generation Of Antigen-specific T-cell Linesmentioning

confidence: 99%

“…These conditions are selective for CD4 cells. The lines, screened for peptide specificity [13], were used after six or more restimulation cycles. Specific proliferation was checked on 2 ϫ 10 4 T cells plus 10 5 autologous irradiated PBMCs in the presence of peptide in duplicate or triplicate wells.…”

Section: Generation Of Antigen-specific T-cell Linesmentioning

confidence: 99%

See 4 more Smart Citations

Human Bone Marrow Stromal Cells Hamper Specific Interactions of CD4 and CD8 T Lymphocytes with Antigen-Presenting Cells

Pira¹,

Ivaldi

Bottone

et al. 2006

Human Immunology

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Section: Hsfs and Monocytes Inhibit T-cell Proliferation Similarly Tomentioning

confidence: 75%

Section: Materials and Methods Antigens And Mediamentioning

confidence: 99%

Section: Generation Of Antigen-specific T-cell Linesmentioning

confidence: 99%

“…PBMC donors were unrelated with BMSC donors. Restimulation cycles were performed every 3 weeks on 2 ϫ 10 5 T cells [12,13]. These conditions are selective for CD4 cells.…”

Section: Generation Of Antigen-specific T-cell Linesmentioning

confidence: 99%

Section: Generation Of Antigen-specific T-cell Linesmentioning

confidence: 99%

See 3 more Smart Citations

Human Bone Marrow Stromal Cells Hamper Specific Interactions of CD4 and CD8 T Lymphocytes with Antigen-Presenting Cells

Pira¹,

Ivaldi

Bottone

et al. 2006

Human Immunology

Self Cite

View full text Add to dashboard Cite

T-cell dysregulation caused by chronic antigenic stress: the role of CMV in immunosenescence?

Pawelec

Gouttefangeas²

2006

Aging Clin Exp Res

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Dysfunctional T-cell immunity contributes to susceptibility to infectious disease in the elderly. A characteristic feature of this "immunosenescence" is the predominance of clonal expansions of CD8 cells and decreased diversity of the T-cell antigen receptor repertoire. Lifelong chronic antigenic stress commonly caused by infection with persistent activating herpes viruses causes the accumulation of anergic, apoptosis-resistant CD8 T cells. These dysfunctional cells are indirectly immunosuppressive by tasking up the "immunological space" as well as directly suppressive via blockade of antigen presenting cells or cytokine secretion. They are associated with an emerging "immunological risk profile" predicting mortality in longitudinal studies of very old people. It is therefore hypothesized that for that majority of elderly people infected with cytomegalovirus (CMV), which seems to act as the dominant chronic stressor, anti-viral strategies would be of benefit in abrogating some of the detrimental clinical manifestations of immunosenescence.

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The immunological burden of human cytomegalovirus infection

Khan

2007

Arch. Immunol. Ther. Exp.

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Cytomegalovirus (CMV) is a persistent DNA virus that has evolved with humans to establish a finely balanced host-virus relationship. This balance is maintained by host immune surveillance since deficiencies in these processes can result in life-threatening disease, as observed in immunologically immature neonates and pharmacologically immunosuppressed transplant recipients. Both T cells and natural killer cells are intimately involved in maintaining asymptomatic infection by specific and non-specific recognition of infected cells. Under pressure from such host immune responses, CMV appears to have evolved elaborate strategies to subvert these responses in order to persist in the host. CMV target antigens are well characterized, with many CD8 T cell and CD4 T cell epitopes reported. This information is now being exploited to treat immunocompromised patients in order to boost virus-specific immunity. This review also discusses our current understanding of how virus carriage may skew lymphocyte populations in immunocompetent subjects and the association of CMV-seropositivity with immunosenescence.

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Recognition of cmv pp65 protein antigen by human cd4 t-cell lines induced with an immunodominant peptide pool

Cited by 10 publications

References 37 publications

Human Bone Marrow Stromal Cells Hamper Specific Interactions of CD4 and CD8 T Lymphocytes with Antigen-Presenting Cells

Human Bone Marrow Stromal Cells Hamper Specific Interactions of CD4 and CD8 T Lymphocytes with Antigen-Presenting Cells

T-cell dysregulation caused by chronic antigenic stress: the role of CMV in immunosenescence?

The immunological burden of human cytomegalovirus infection

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